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High-throughput droplet sorting devices for large-scale single-cell analysis

Research Project

Project/Area Number 18K14101
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 28050:Nano/micro-systems-related
Research InstitutionThe University of Tokyo

Principal Investigator

Isozaki Akihiro  東京大学, 大学院理学系研究科(理学部), 特任助教 (10732555)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
KeywordsDroplet microfluidics / Single-cell analysis / マイクロ流路 / Flow cytometry / 液滴 / 抗体産生 / 創薬
Outline of Final Research Achievements

I have developed a high-speed large droplet manipulation method that is an order of magnitude faster than conventional methods. Specifically, by precisely controlling the timing and position of the force applied to droplets, the developed method enables gentle and fast manipulation of fragile, large droplets, resulting in a 20-fold increase in the manipulation speed compared to conventional methods. Furthermore, I have demonstrated that microalgal cells can move freely in droplets larger than 100 pL, which is an advantage of our method. The survival rate of their microalgal cells and leukemia cells is increased as well as the antibody production of hybridoma cells. Furthermore, I have used it to isolate microorganisms with a slow growth rate, which only exist in about 2% of the population, confirming its usefulness and versatility.

Academic Significance and Societal Importance of the Research Achievements

液滴分取スピードの高速化により、これまで見つけられなかったような希少な細胞を発見・単離できる可能性が大幅に向上する。これにより、微生物学、免疫学、遺伝学などの基礎科学における新たな発見や、精密医療、ウイルス検査、創薬、バイオ燃料開発、スマートセル産業などにおける効率化技術の開発といった、さまざまな応用展開が期待される。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (14 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (1 results) Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (10 results) (of which Int'l Joint Research: 2 results) Remarks (1 results)

  • [Int'l Joint Research] LMU München(ドイツ)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Dual sequentially addressable dielectrophoretic array for high-throughput, scalable, multiplexed droplet sorting2021

    • Author(s)
      Isozaki Akihiro、Huang Dunhou、Nakagawa Yuta、Goda Keisuke
    • Journal Title

      Microfluidics and Nanofluidics

      Volume: 25 Issue: 4 Pages: 1-10

    • DOI

      10.1007/s10404-021-02432-z

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sequentially addressable dielectrophoretic array for high-throughput sorting of large-volume biological compartments2020

    • Author(s)
      A Isozaki, Y Nakagawa, M H Loo, Y Shibata, N Tanaka, D L Setyaningrum, J-W Park, Y Shirasaki, H Mikami, D Huang, H Tsoi, C T Riche, T Ota, H Miwa, Y Kanda, T Ito, K Yamada, O Iwata, K Suzuki, S Ohnuki, Y Ohya, Y Kato, T Hasunuma, S Matsusaka, M Yamagishi, M Yazawa, S Uemura, K Nagasawa, H Watarai, D Di Carlo, K Goda
    • Journal Title

      Science Advances

      Volume: 29 Issue: 22 Pages: 1-11

    • DOI

      10.1126/sciadv.aba6712

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] "High-throuhgput sorting of nanoliter droplets using an electrode array with a slanted microchannel2020

    • Author(s)
      Mun Hong Loo, Yuta Nakagawa, Akihiro Isozaki, and Keisuke Goda
    • Organizer
      The 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS2020)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Sequentially addressable dielectrophoretic array for high-throughput large-droplet sorting2020

    • Author(s)
      Akihiro Isozaki, Yuta Nakagawa, Mun Hong Loo, Dino Di Carlo, and Keisuke Goda
    • Organizer
      CYTO Virtual 2020
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Exploring the advantage of large droplets for single-cell analysis2020

    • Author(s)
      Yuta Nakagawa, Shinsuke Ohnuki, Akihiro Isozaki, Naoko Kondo, Yoshikazu Ohya, and Keisuke Goda
    • Organizer
      化学とマイクロ・ナノシステム学会第42回研究会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 電極アレイを用いた高速液滴分取2019

    • Author(s)
      磯崎 瑛宏、中川 悠太、Mun Hong Loo、芝田 悠大、田中 直樹、Dwi Larasati Setyaningrum、Jee-Woong Park、白崎 善隆、三上 秀治、上村 想太郎、Dino Di Carlo、合田圭介
    • Organizer
      化学とマイクロ・ナノシステム学会
    • Related Report
      2019 Research-status Report
  • [Presentation] ow-loss droplet picking for high-throughput rare-cell sorting2019

    • Author(s)
      Chenqi Zhang, Mun Houng Loo, Yuta Nakagawa, Akihiro Isozaki, Keisuke Goda
    • Organizer
      化学とマイクロ・ナノシステム学会
    • Related Report
      2019 Research-status Report
  • [Presentation] A droplet microfluidic platform for separation of cells with low proliferative activity2019

    • Author(s)
      Yuta Nakagawa, Mun Hong Loo, Shinsuke Ohnuki, Yoshikazu Ohya, Akihiro Isozaki, Keisuke Goda
    • Organizer
      化学とマイクロ・ナノシステム学会
    • Related Report
      2019 Research-status Report
  • [Presentation] 電極アレイを用いた多分岐高速液滴分取2019

    • Author(s)
      磯崎 瑛宏, Dunhou Huang, 合田 圭介
    • Organizer
      化学とマイクロ・ナノシステム学会
    • Related Report
      2019 Research-status Report
  • [Presentation] 電極アレイを用いた超高速液滴分取とその応用2019

    • Author(s)
      中川 悠太
    • Organizer
      日本化学会春季年会
    • Related Report
      2018 Research-status Report
  • [Presentation] An electrode-array-based high-throughput nano-liter-droplet sorter assisted by a slanted microchannel2019

    • Author(s)
      Loo Mun Hong
    • Organizer
      日本化学会春季年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 微小液滴内におけるハイブリドーマの抗体産生量2018

    • Author(s)
      磯崎瑛宏
    • Organizer
      化学とマイクロ・ナノシステム学会第38回研究会
    • Related Report
      2018 Research-status Report
  • [Remarks] Akihiro Isozaki

    • URL

      https://sites.google.com/site/akihiroisozaki/

    • Related Report
      2020 Annual Research Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

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