Exploration of enzymatic reaction systems for measurement using graphene transistor and their application to enzyme immunoassay
| Project/Area Number |
18K14107
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 28050:Nano/micro-systems-related
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| Research Institution | Osaka University |
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Principal Investigator |
Ono Takao 大阪大学, 産業科学研究所, 助教 (00752875)
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| Project Period (FY) |
2018-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | グラフェン / 酵素反応 / 電界効果トランジスタ / マイクロ流体デバイス / デバイ長 / 病原体 / グラフェントランジスタ / マイクロウェル / 酵素免疫法 / ウイルス / 実時間反応計測 / 酵素 / バイオセンシング / 微小液滴 / ELISA / インフルエンザウイルス / バイオセンサー / 酵素反応計測 |
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| Outline of Final Research Achievements |
In this study, I extended an application field of my high-sensitive electrical biosensing method using graphene field-effect transisors (graphene-FET) and microdroplets. It is a part of my "lab on a graphene-FET" concept, a novel platform for sensing various biochemical reactions using graphene-FETs. I found a new enzymatic reaction system which is more commonly used compared to urease/urea reaction system and able to be measured by graphene-FETs. I applied this system to enzyme immunoassay and detected target-specific signals from influenza virus.
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| Academic Significance and Societal Importance of the Research Achievements |
研究代表者が原理実証した、「酵素反応産物による電気的な生体分子検出」と「反応系のマイクロメートルスケールへの封じ込め」による高感度なバイオセンシング技術の実用化に向けて、酵素反応系の改変と抗体との複合化によって、技術の適用範囲を拡げることが出来た。これらを通じて、研究代表者の提唱する"lab on a graphene-FET"の可能性を拡げることが出来た。
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Report
(3 results)
Research Products
(34 results)
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[Presentation] Detection of Human-Infectious Influenza Virus Using Sialoglycan-Modified Graphene Field-Effect Transistor2018
Author(s)
Takao Ono, Takuya Kawata, Shota Ushiba, Yasushi Kanai, Yasuhide Ohno, Kenzo Maehashi, Koichi Inoue, Yohei Watanabe, Shin’ichi Nakakita, Yasuo Suzuki, Toshio Kawahara, Masahiko Kimura, and Kazuhiko Matsumoto
Organizer
2018 International Conference on Solid State Devices and Materials
Related Report
Int'l Joint Research
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