• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of Simple, Rapid Discrimination Method of Bacteria by Boronic Acid Modified Polymer

Research Project

Project/Area Number 18K14255
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 34020:Analytical chemistry-related
Research InstitutionWaseda University (2019-2021)
Institute of Physical and Chemical Research (2018)

Principal Investigator

TSUCHIDO YUJI  早稲田大学, 理工学術院, 講師(任期付) (40737219)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords細菌 / 分子認識 / ボロン酸 / デンドリマー / 超分子 / ナノ材料 / 高感度 / 簡易判別 / 迅速判別 / 凝集 / 蛍光 / 糖 / Molecular Recognition / Bacteria / Boronic Acid / Saccharide / Dendrimer / Phenylboronic Acid / ナノ粒子
Outline of Final Research Achievements

We developed novel detection method of bacteria by using boronic acid modified dendrimer. This method is a quite simple method by only mixing bacteria solution and boronic acid dendrimer solution. The mixture forms large aggregates. The detection sensitivity is about 1,000,000 CFU/mL for the turbidity method and 10,000 CFU/mL for fluorescence method. The detection time was about 5 minutes to several tens of minutes. Rapid detection of bacteria was achieved in a significantly shorter time than the conventional method.

Academic Significance and Societal Importance of the Research Achievements

細菌やウイルスをはじめとした、病原性微生物のパンデミックが大きな社会問題となっており、病原性微生物を簡易迅速に検出する手法の開発が強く望まれている。本研究では、分子認識反応で細菌表面と結合して凝集させることによって、光の散乱や蛍光で細菌を検出して簡易判別する手法を開発した。この成果は学術的な意義のみならず、試薬と光源があれば、場所を問わずに迅速に細菌を検出できる手法であることから、SDGsの面でも大きな社会的意義がある。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (45 results)

All 2022 2021 2020 2019 2018 Other

All Journal Article (11 results) (of which Peer Reviewed: 10 results,  Open Access: 9 results) Presentation (30 results) (of which Int'l Joint Research: 9 results,  Invited: 1 results) Remarks (4 results)

  • [Journal Article] Rapid Bacterial Recognition over a Wide pH Range by Boronic Acid-Based Ditopic Dendrimer Probes for Gram-Positive Bacteria2022

    • Author(s)
      Ayame Mikagi, Koichi Manita, Asuka Yoyasu, Yuji Tsuchido, Nobuyuki Kanzawa, Takeshi Hashimoto, and Takashi Hayashita
    • Journal Title

      Molecules

      Volume: 27 Pages: 256-256

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of Spacer Length in Pyrene-Modified-Phenylboronic Acid Probe/CyD Complexes on Fluorescence-based Recognition of Monosaccharides in Aqueous Solution2021

    • Author(s)
      TSUCHIDO Yuji、KOJIMA Shohei、SUGITA Ko、FUJIWARA Shoji、HASHIMOTO Takeshi、HAYASHITA Takashi
    • Journal Title

      Analytical Sciences

      Volume: 37 Issue: 5 Pages: 721-726

    • DOI

      10.2116/analsci.20SCP08

    • NAID

      130008036603

    • ISSN
      0910-6340, 1348-2246
    • Year and Date
      2021-05-10
    • Related Report
      2021 Annual Research Report 2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fast and Sensitive Bacteria Detection by Boronic Acid Modified Fluorescent Dendrimer2021

    • Author(s)
      Mikagi Ayame、Tsurufusa Riho、Tsuchido Yuji、Hashimoto Takeshi、Hayashita Takashi
    • Journal Title

      Sensors

      Volume: 21 Issue: 9 Pages: 3115-3115

    • DOI

      10.3390/s21093115

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Micelle-Type Sensor for Saccharide Recognition by Using Boronic Acid Fluorescence Amphiphilic Probe and Surfactants2021

    • Author(s)
      Tsuchido Yuji、Nodomi Nana、Hashimoto Takeshi、Hayashita Takashi
    • Journal Title

      Solvent Extraction and Ion Exchange

      Volume: - Issue: 5-6 Pages: 668-677

    • DOI

      10.1080/07366299.2021.1876988

    • Related Report
      2021 Annual Research Report 2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Structural Effect of Fluorophore on Phenylboronic Acid Fluorophore/Cyclodextrin Complex for Selective Glucose Recognition2020

    • Author(s)
      Takeshi Hashimoto, Mio Kumai, Mariko Maeda, Koji Miyoshi, Yuji Tsuchido, Shoji Fujiwara, and Takashi Hayashita
    • Journal Title

      Front. Chem. Sci. Eng.

      Volume: 14 Issue: 1 Pages: 53-60

    • DOI

      10.1007/s11705-019-1851-y

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Selective Sugar Recognition by Anthracene-Type Boronic Acid Fluorophore/Cyclodextrin Supramolecular Complex Under Physiological pH Condition2019

    • Author(s)
      Sugita Ko、Tsuchido Yuji、Kasahara Chisato、Casulli Maria Antonietta、Fujiwara Shoji、Hashimoto Takeshi、Hayashita Takashi
    • Journal Title

      Frontiers in Chemistry

      Volume: 7 Pages: 806-812

    • DOI

      10.3389/fchem.2019.00806

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Rapid and Selective Discrimination of Gram-Positive and Gram-Negative Bacteria by Boronic Acid-Modified Poly(amidoamine) Dendrimer2019

    • Author(s)
      Tsuchido Yuji、Horiuchi Ryosuke、Hashimoto Takeshi、Ishihara Kanako、Kanzawa Nobuyuki、Hayashita Takashi
    • Journal Title

      Analytical Chemistry

      Volume: 91 Issue: 6 Pages: 3929-3935

    • DOI

      10.1021/acs.analchem.8b04870

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Metal and Phosphate Ion Recognition Using Dipicolylamine-modified Fluorescent Silica Nanoparticles2018

    • Author(s)
      TSUCHIDO Yuji、YAMASAWA Aya、HASHIMOTO Takeshi、HAYASHITA Takashi
    • Journal Title

      Analytical Sciences

      Volume: 34 Issue: 10 Pages: 1125-1130

    • DOI

      10.2116/analsci.18P153

    • NAID

      130007496572

    • ISSN
      0910-6340, 1348-2246
    • Year and Date
      2018-10-10
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Design of Saccharide Recognition Material Based on Boronic Acid Fluorophore/Cyclodextrin Gel2018

    • Author(s)
      SOMA Suzuka、SUZUKI Takahito、G. BEKELE Tewodros、TSUCHIDO Yuji、HASHIMOTO Takeshi、HAYASHITA Takashi
    • Journal Title

      Journal of Ion Exchange

      Volume: 29 Issue: 3 Pages: 126-130

    • DOI

      10.5182/jaie.29.126

    • NAID

      130007485257

    • ISSN
      0915-860X, 1884-3360
    • Year and Date
      2018-09-20
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Design and Function of Fluorescent Silica Nanoparticles for Bacteria Detection2018

    • Author(s)
      KITAMURA Aya、KASAI Yuna、TSUCHIDO Yuji、HASHIMOTO Takeshi、HAYASHITA Takashi
    • Journal Title

      Journal of Ion Exchange

      Volume: 29 Issue: 3 Pages: 121-125

    • DOI

      10.5182/jaie.29.121

    • NAID

      130007485160

    • ISSN
      0915-860X, 1884-3360
    • Year and Date
      2018-09-20
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 分子認識プローブ修飾ナノ粒子を用いた細菌検出法の開発2018

    • Author(s)
      土戸優志
    • Journal Title

      バイオマテリアル―生体材料―

      Volume: 37 Pages: 18-19

    • Related Report
      2018 Research-status Report
  • [Presentation] ジトピック型ボロン酸デンドリマーによる細菌認識法の開発2022

    • Author(s)
      〇三ヶ木彩芽・眞仁田晃一・与安明日香・土戸優志・橋本剛・神澤信行・早下隆士
    • Organizer
      第19回ホスト-ゲスト・超分子化学シンポジウム
    • Related Report
      2021 Annual Research Report
  • [Presentation] Design of boronic acid modified polyamidoamine dendrimer for rapid and selective discrimination of Gram-positive bacteria2021

    • Author(s)
      Yuji Tsuchido, Riho Tsurufusa, Masaru Ikeno, Ayame Mikagi, Takeshi Hashimoto, Takashi Hayashita
    • Organizer
      The International Chemical Congress of Pacific Basin Societies 2021 (Pacifichem 2021)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A novel convenient and sensitive detection for bacteria by fluorescent labelled dendrimers with boronic acid2021

    • Author(s)
      Ayame Mikagi, Riho Tsurufusa, Yuji Tsuchido, Takeshi Hashimoto, Takashi Hayashita
    • Organizer
      The International Chemical Congress of Pacific Basin Societies 2021 (Pacifichem 2021)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Selective sugar recognition using phenylboronic acid conjugated fluorophore/cyclodextrin complexes2021

    • Author(s)
      Takeshi Hashimoto, Maria Antonietta Casulli, Shoji Fujiwara, Yuji Tsuchido, Takashi Hayashita
    • Organizer
      The International Chemical Congress of Pacific Basin Societies 2021 (Pacifichem 2021)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Structural Effect on Phenylboronic Acid Fluorophore/Cyclodextrin Complex for Selective Sugar Recognition2021

    • Author(s)
      Takeshi Hashimoto, Yuji Tsuchido, Shoji Fujiwara, Takashi Hayashita
    • Organizer
      Asian Conference on Analytical Sciences 2020 (ASIANALYSIS 2021)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ボロン酸修飾PAMAMデンドリマーを用いた細菌認識法の開発2021

    • Author(s)
      〇三ヶ木彩芽・鶴房莉帆・土戸優志・橋本剛・早下隆士
    • Organizer
      第35回日本イオン交換研究発表会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ダンシル修飾フェニルボロン酸デンドリマーを用いた高感度細菌検出法の開発2021

    • Author(s)
      〇三ヶ木彩芽・鶴房莉帆・土戸優志・橋本剛・早下隆士
    • Organizer
      日本分析化学会第70年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ボロン酸修飾デンドリマーを用いた蛍光測定による高感度細菌認識法の開発2021

    • Author(s)
      〇三ヶ木彩芽・鶴房莉帆・土戸優志・橋本剛・早下隆士
    • Organizer
      第18回ホスト-ゲスト・超分子化学シンポジウム
    • Related Report
      2021 Annual Research Report
  • [Presentation] ダンシル蛍光団を有するフェニルボロン酸デンドリマーを用いた高感度細菌認識法の開発2021

    • Author(s)
      〇三ヶ木彩芽・鶴房莉帆・土戸優志・橋本剛・早下隆士
    • Organizer
      日本化学会第101春季年会
    • Related Report
      2020 Research-status Report
  • [Presentation] 細菌種の簡易識別を可能としたボロン酸修飾ポリマーの分子設計と機能評価2019

    • Author(s)
      ○土戸優志・鶴房莉帆・与安明日香・池野知・橋本剛・早下隆士
    • Organizer
      第68回高分子討論会
    • Related Report
      2019 Research-status Report
  • [Presentation] ピレン型蛍光プローブ/シクロデキストリン複合体の構造効果2019

    • Author(s)
      ○笠原千聖・水田祐司・土戸優志・橋本剛・早下隆士
    • Organizer
      第36回シクロデキストリンシンポジウム
    • Related Report
      2019 Research-status Report
  • [Presentation] 細菌種の簡易識別を可能としたボロン酸修飾デンドリマーの分子設計と機能評価2019

    • Author(s)
      ○土戸優志・鶴房莉帆・与安明日香・池野知・橋本剛・早下隆士
    • Organizer
      日本分析化学会第68年会
    • Related Report
      2019 Research-status Report
  • [Presentation] デンドリマー型フェニルボロン酸細菌検出プローブの分子設計2019

    • Author(s)
      ○鶴房莉帆・池野知・土戸優志・橋本剛・早下隆士
    • Organizer
      日本分析化学会第68年会
    • Related Report
      2019 Research-status Report
  • [Presentation] ボロン酸型蛍光プローブ修飾ポリマーによるバイオフィルム検出能評価2019

    • Author(s)
      ○藤澤真友子・福島学・土戸優志・橋本剛・早下隆士
    • Organizer
      日本分析化学会第68年会
    • Related Report
      2019 Research-status Report
  • [Presentation] ボロン酸型プローブ/デンドリマー複合体による細菌検出におけるベタイン導入効果2019

    • Author(s)
      ○与安明日香・喜多村文・土戸優志・橋本剛・早下隆士
    • Organizer
      令和元年度日本分析化学会関東支部若手交流会
    • Related Report
      2019 Research-status Report
  • [Presentation] ボロン酸型蛍光プローブ修飾ポリマーの開発とバイオフィルム検出能評価2019

    • Author(s)
      ○藤澤真友子・福島学・土戸優志・橋本剛・早下隆士
    • Organizer
      令和元年度日本分析化学会関東支部若手交流会
    • Related Report
      2019 Research-status Report
  • [Presentation] ボロン酸修飾デンドリマーの細菌応答機能評価2019

    • Author(s)
      ○土戸優志・池野知・鶴房莉帆・堀内良介・橋本剛・早下隆士
    • Organizer
      第68回高分子学会年次大会
    • Related Report
      2019 Research-status Report
  • [Presentation] ボロン酸修飾デンドリマーの細菌識別機能評価2019

    • Author(s)
      ○土戸優志・池野知・鶴房莉帆・堀内良介・橋本剛・早下隆士
    • Organizer
      第79回分析化学討論会
    • Related Report
      2019 Research-status Report
  • [Presentation] 細菌認識能を有するボロン酸型蛍光プローブの開発2018

    • Author(s)
      ○喜多村 文・笠井 祐那・土戸 優志・橋本 剛・早下 隆士
    • Organizer
      第31回イオン交換セミナー
    • Related Report
      2018 Research-status Report
  • [Presentation] ボロン酸型蛍光プローブ修飾ポリマーの開発とバイオフィルム検出2018

    • Author(s)
      〇藤澤 真友子・福島 学・土戸 優志・橋本 剛・早下 隆士
    • Organizer
      第31回イオン交換セミナー
    • Related Report
      2018 Research-status Report
  • [Presentation] Development of Functional Nanoparticles for The Detection of Bacteria2018

    • Author(s)
      Yuji Tsuchido, Aya Kitamura, Yuna Kasai, Hiroyuki Kobayashi, Takeshi Hashimoto, Takashi Hayashita
    • Organizer
      第40回日本バイオマテリアル学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] ボロン酸型蛍光プローブ修飾ポリマーの設計とバイオフィルム検出2018

    • Author(s)
      〇藤澤 真友子・福島 学・土戸 優志・橋本 剛・早下 隆士
    • Organizer
      第8回CSJ化学フェスタ2018
    • Related Report
      2018 Research-status Report
  • [Presentation] Development of Functional Nanoparticles for The Detection of Bacteria2018

    • Author(s)
      Yuji Tsuchido, Aya Kitamura, Yuna Kasai, Hiroyuki Kobayashi, Takeshi Hashimoto, Takashi Hayashita
    • Organizer
      2018 Fall Meeting of The Korean Society for Biomaterials
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Design and Function of Fluorescent Silica Nanoparticles for Bacteria Detection2018

    • Author(s)
      Aya Kitamura, Yuna Kasai, Yuji Tsuchido, Takeshi Hashimoto, Takashi Hayashita
    • Organizer
      7th International Conference on Ion Exchange 2018 (ICIE2018)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] The Effect of Modification Ratio to Bacteria Detection Using Dendrimer/Phenylboronic Acid Complexes2018

    • Author(s)
      Masaru Ikeno, Ryosuke Horiuchi, Yuji Tsuchido, Takeshi Hashimoto, Takashi Hayashita
    • Organizer
      7th International Conference on Ion Exchange 2018 (ICIE2018)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Design of Saccharide Recognition Material Based on Boronic Acid Fluorophore/Cyclodextrin Gel2018

    • Author(s)
      Suzuka Soma, Takahito Suzuki, Tewodros Getachew Bekele, Yuji Tsuchido, Takeshi Hashimoto, Takashi Hayashita
    • Organizer
      7th International Conference on Ion Exchange 2018 (ICIE2018)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] ボロン酸修飾デンドリマーを用いたグラム陽性菌の選択的認識2018

    • Author(s)
      ○土戸 優志・堀内 良介・池野 知・橋本 剛・早下 隆士
    • Organizer
      第16回ホスト-ゲスト・超分子化学シンポジウム
    • Related Report
      2018 Research-status Report
  • [Presentation] アゾ基を有するデンドリマー型フェニルボロン酸による細菌認識2018

    • Author(s)
      ○鶴房 莉帆・池野 知・土戸 優志・橋本 剛・早下 隆士
    • Organizer
      第16回ホスト-ゲスト・超分子化学シンポジウム
    • Related Report
      2018 Research-status Report
  • [Presentation] ボロン酸型蛍光プローブ修飾ポリマーの設計とバイオフィルム検出2018

    • Author(s)
      ○藤澤 真友子・福島 学・土戸 優志・橋本 剛・早下 隆士
    • Organizer
      第78回分析化学討論会
    • Related Report
      2018 Research-status Report
  • [Presentation] Evaluation of Fluorescent Probes Possessing Halogen for Saccharides Recognition in Water2018

    • Author(s)
      Chisato Kasahara, Shohei Kojima, Yuji Mizuta, Ko Sugita, Yuji Tsuchido, Takeshi Hashimoto, Takashi Hayashita
    • Organizer
      The 19th International Cyclodextrin Symposium (ICS 2018)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Remarks] researchmap

    • URL

      https://researchmap.jp/yuji-tsuchido

    • Related Report
      2021 Annual Research Report
  • [Remarks] researchmap

    • URL

      https://researchmap.jp/yuji-tsuchido/

    • Related Report
      2020 Research-status Report 2019 Research-status Report
  • [Remarks] 上智大学理工学部物質生命理工学科 分析化学グループ ホームページ

    • URL

      http://www.mls.sophia.ac.jp/~analysis/index.html

    • Related Report
      2018 Research-status Report
  • [Remarks] researchmap

    • URL

      https://researchmap.jp/7000007573

    • Related Report
      2018 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2023-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi