| Project/Area Number |
18K14255
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 34020:Analytical chemistry-related
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| Research Institution | Waseda University (2019-2021)
Institute of Physical and Chemical Research (2018) |
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Principal Investigator |
TSUCHIDO YUJI 早稲田大学, 理工学術院, 講師(任期付) (40737219)
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 細菌 / 分子認識 / ボロン酸 / デンドリマー / 超分子 / ナノ材料 / 高感度 / 簡易判別 / 迅速判別 / 凝集 / 蛍光 / 糖 / Molecular Recognition / Bacteria / Boronic Acid / Saccharide / Dendrimer / Phenylboronic Acid / ナノ粒子 |
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| Outline of Final Research Achievements |
We developed novel detection method of bacteria by using boronic acid modified dendrimer. This method is a quite simple method by only mixing bacteria solution and boronic acid dendrimer solution. The mixture forms large aggregates. The detection sensitivity is about 1,000,000 CFU/mL for the turbidity method and 10,000 CFU/mL for fluorescence method. The detection time was about 5 minutes to several tens of minutes. Rapid detection of bacteria was achieved in a significantly shorter time than the conventional method.
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| Academic Significance and Societal Importance of the Research Achievements |
細菌やウイルスをはじめとした、病原性微生物のパンデミックが大きな社会問題となっており、病原性微生物を簡易迅速に検出する手法の開発が強く望まれている。本研究では、分子認識反応で細菌表面と結合して凝集させることによって、光の散乱や蛍光で細菌を検出して簡易判別する手法を開発した。この成果は学術的な意義のみならず、試薬と光源があれば、場所を問わずに迅速に細菌を検出できる手法であることから、SDGsの面でも大きな社会的意義がある。
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