| Project/Area Number |
18K14260
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 34020:Analytical chemistry-related
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| Research Institution | Japan Agency for Marine-Earth Science and Technology |
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Principal Investigator |
Zhang Yi 国立研究開発法人海洋研究開発機構, 超先鋭研究開発部門(超先鋭研究プログラム), 研究員 (40795358)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | High throughput / Microfabrication / Single molecule / Enzyme / Sensitivity / Poisson statistics / Image processing / Screening / Restriction enzyme / Electrophoresis / Digital counting / Droplet / Biosensor / Cell-free / Directed evolution / Synthetic biology / PCR / Protein synthesis / Micromanipulation / DNA / Bioreactor / Microdevice |
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| Outline of Final Research Achievements |
We cannot study what we cannot measure. This simple philosophy is the basic driving force for analytical chemistry to move forward. This Early-Career Scientists study was also planned and implemented based on this general interest. My research platform features high throughput and deals with a massive number of single DNA molecules, which enabled: (1) cell-free protein synthesis from every single DNA molecule; (2) genotype-phenotype linkage at the single DNA molecule; (3) sorting and amplification for single DNA molecules; and (4) amplification-free single DNA molecule detection. During the period of this research, I succeeded in obtaining the world's most active enzyme capable of hydrolyzing phosphate esters, establishing the novel digital high-throughput screening theory, and developing the world's most sensitive method capable of evaluating the performance of any DNA-cleaving enzymes.
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| Academic Significance and Societal Importance of the Research Achievements |
The massively parallel, highly uniform, and individually addressable femtoliter droplet array technology offered a high-end standard and new possibilities for future diverse bioanalytical applications. The integrated system also stressed the significance of the multidisciplinary approach.
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