| Project/Area Number |
18K14270
|
|---|
| Research Category |
Grant-in-Aid for Early-Career Scientists
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
Basic Section 35010:Polymer chemistry-related
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
P. K. Hashim 東京大学, 大学院工学系研究科(工学部), 特任研究員 (40817277)
|
|---|
| Project Period (FY) |
2018-04-01 – 2020-03-31
|
| Project Status |
Completed (Fiscal Year 2019)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | DNA nanostructure / Drug delivery system / Template polymerization / Adhesion / peptide conjugation / DNA origami / Nanostructure / Guanidinium ion / Click reaction / DNA Functionalization / DNA nanostructure / Molecular glue / click reaction / functionalization of DNA / Polymer coating / DNA origami structures / Drug Delivery system / in vivo stability of DDS |
|---|
| Outline of Final Research Achievements |
For stabilization and functionalization of DNA-nanostructures, two macromonomers comprising guanidinium ion (Gu+) at their main chain or side chain, and azide units were synthesized. Upon mixing with a DNA origami 6-helix bundle, the macromonomers successfully adhered to the phosphate groups of exposed helices of DNA, however, the solubility of resulting macromonomer/origami conjugate was poor. The solubility issue was partly solved upon using the macromonomer with a more flexible backbone and benzophenone (BP) motif. We systematically investigated how the monomer interacts with DNA using a 40-base pair DNA. Our preliminary data also suggested that the glue coated 40-base pair DNA can be further functionalized with proteins.
|
| Academic Significance and Societal Importance of the Research Achievements |
我々はDNAナノ構造の被覆および機能化のため、DNAへの接着部位としてグアニジニウムイオン Gu+を有する分子の開発に取り組んでいます。 より簡単な構造である40塩基対のDNAを使用し、合成した分子とDNA間に働く相互作用について体系的な調査を行いました。DNAナノ構造はドラッグデリバリーシステムにおける輸送体として実用化が期待されていますが、血中で酵素による分解を急速に受けるため、DNAナノ構造の表面を被覆し生物学的な機能を付与することが必要です。 我々が開発してきた、Gu+含有分子を用いたDNAナノ構造の被覆・機能化の技術は、低毒性かつ血中で安定な輸送体の開発に貢献する可能性を有しています
|
