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Semi-synthesis of photo-switchable nanopore BAX protein

Research Project

Project/Area Number 18K14337
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 37010:Bio-related chemistry
Research InstitutionKyoto Pharmaceutical University

Principal Investigator

Asahina Hiroko  京都薬科大学, 薬学部, 助教 (90808461)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywordsナノポア / 半合成 / BAX / ペプチド化学 / タンパク質工学 / 膜タンパク質 / バイオテクノロジー
Outline of Final Research Achievements

In this study, we tried to semi-synthesize photo-switchable nanopore BAX protein. The protein is semi-synthesized by Native Chemical Ligation of the N-terminal BAX protein segment and the C-terminal peptide segment including azobenzene amino acid. The C-terminal peptide segment was chemical synthesized and its Cis/Trans conformation change using LED light source was detected by UV/Vis measurement. However, the enough amount of BAX protein segment for ligation was not obtained by E.coli expression which was established before. Now, we continue to prepare and analyze the sample.

Academic Significance and Societal Importance of the Research Achievements

ナノマシンの駆動原理を用いてナノポアの開閉を行う先行研究はあるが、いずれも小分子化合物が通過する程度であり、また、その孔は脂質二重膜に開いたままで閉ざすものではない。本研究の光感受性人工BAXはタンパク質が通過する十分な大きさを持つナノポアを形成することが可能であり、閉孔と膜からの解離を制御することができると考えらる。まだ完成には至っていないが、この光感受性人工BAXは人工脂質二重膜や生体細胞膜の開閉分子として、細胞生物学や構造生物学など様々な分野のケミカルツールとして活用できるだけでなく、新たなドラッグデリバリーシステムとしても役立つと期待できる。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (6 results)

All 2022 2019 2018

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (4 results) (of which Invited: 1 results)

  • [Journal Article] Effect of hydrophobic moment on membrane interaction and cell penetration of apolipoprotein E‐derived arginine‐rich amphipathic α‐helical peptides2022

    • Author(s)
      Yuki Takechi‐Haraya, Takashi Ohgita, Mana Kotani, Hiroki Kono, Chihiro Saito, Hiroko Tamagaki‐Asahina, Kazuchika Nishitsuji, Kenji Uchimura, Takeshi Sato, Ryuji Kawano, Kumiko Sakai‐Kato, Ken‐ichi Izutsu and Hiroyuki Saito
    • Journal Title

      Scientific Reports

      Volume: 12 Issue: 1 Pages: 4959-4959

    • DOI

      10.1038/s41598-022-08876-9

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Catalytic site-selective substrate processing within a tubular nanoreactor2019

    • Author(s)
      Qing Yujia、Tamagaki-Asahina Hiroko、Ionescu Sandra A.、Liu Mira D.、Bayley Hagan
    • Journal Title

      Nature Nanotechnology

      Volume: 14 Issue: 12 Pages: 1135-1142

    • DOI

      10.1038/s41565-019-0579-7

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Conserved tyrosine residues involve in the orientation of the transmembrane region in FGFR32019

    • Author(s)
      Hiroko Tamagaki-Asahina
    • Organizer
      第56回ペプチド討論会
    • Related Report
      2019 Research-status Report
  • [Presentation] FGFR3の膜貫通部位に存在するチロシン残基と膜貫通部位の配向2019

    • Author(s)
      朝比奈(玉垣)裕子
    • Organizer
      第92回日本生化学会
    • Related Report
      2019 Research-status Report
  • [Presentation] ペプチド化学を用いた線維芽細胞増殖因子受容体3の構造機能解析2019

    • Author(s)
      玉垣(朝比奈)裕子
    • Organizer
      蛋白研セミナー
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 線維芽細胞増殖因子受容体3の膜貫通部位に存在するチロシンによる配向決定2018

    • Author(s)
      玉垣(朝比奈)裕子
    • Organizer
      日本蛋白質科学会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2023-01-30  

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