Semi-synthesis of photo-switchable nanopore BAX protein
| Project/Area Number |
18K14337
|
|---|
| Research Category |
Grant-in-Aid for Early-Career Scientists
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
Basic Section 37010:Bio-related chemistry
|
|---|
| Research Institution | Kyoto Pharmaceutical University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2018-04-01 – 2022-03-31
|
| Project Status |
Completed (Fiscal Year 2021)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
|---|
| Keywords | ナノポア / 半合成 / BAX / ペプチド化学 / タンパク質工学 / 膜タンパク質 / バイオテクノロジー |
|---|
| Outline of Final Research Achievements |
In this study, we tried to semi-synthesize photo-switchable nanopore BAX protein. The protein is semi-synthesized by Native Chemical Ligation of the N-terminal BAX protein segment and the C-terminal peptide segment including azobenzene amino acid. The C-terminal peptide segment was chemical synthesized and its Cis/Trans conformation change using LED light source was detected by UV/Vis measurement. However, the enough amount of BAX protein segment for ligation was not obtained by E.coli expression which was established before. Now, we continue to prepare and analyze the sample.
|
| Academic Significance and Societal Importance of the Research Achievements |
ナノマシンの駆動原理を用いてナノポアの開閉を行う先行研究はあるが、いずれも小分子化合物が通過する程度であり、また、その孔は脂質二重膜に開いたままで閉ざすものではない。本研究の光感受性人工BAXはタンパク質が通過する十分な大きさを持つナノポアを形成することが可能であり、閉孔と膜からの解離を制御することができると考えらる。まだ完成には至っていないが、この光感受性人工BAXは人工脂質二重膜や生体細胞膜の開閉分子として、細胞生物学や構造生物学など様々な分野のケミカルツールとして活用できるだけでなく、新たなドラッグデリバリーシステムとしても役立つと期待できる。
|
Report
(5 results)
Research Products
(6 results)
-
[Journal Article] Effect of hydrophobic moment on membrane interaction and cell penetration of apolipoprotein E‐derived arginine‐rich amphipathic α‐helical peptides2022
Author(s)
Yuki Takechi‐Haraya, Takashi Ohgita, Mana Kotani, Hiroki Kono, Chihiro Saito, Hiroko Tamagaki‐Asahina, Kazuchika Nishitsuji, Kenji Uchimura, Takeshi Sato, Ryuji Kawano, Kumiko Sakai‐Kato, Ken‐ichi Izutsu and Hiroyuki Saito
-
Journal Title
Scientific Reports
Volume: 12
Issue: 1
Pages: 4959-4959
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-
-
