Analysis of mechanisms of circular RNA translation in eukaryotic systems
| Project/Area Number |
18K14357
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 37030:Chemical biology-related
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| Research Institution | Nagoya University |
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Principal Investigator |
Abe Naoko 名古屋大学, 理学研究科, 特任助教 (70772119)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 環状RNA / 翻訳反応 / RNA / 環状構造 / 真核細胞翻訳系 / 環状 / 翻訳 |
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| Outline of Final Research Achievements |
1. Sequence dependence of the circular RNA translation was revealed. 2. It was shown that the translation efficiency of linear mRNA having a pseudocyclic structure was improved. 3. A synthetic method of chemically linking circular RNA was examined, and it was shown that mRNA containing unnatural linkage could be translated. 4. The mRNA in which the phosphorothioate modification was introduced in the 5'untranslated region in a limited manner greatly improved the translation activity in the E. coli cell-free translation system. This indicates that the introduction of position-specific chemical modification is an effective method for improving the translational activity of RNA.
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| Academic Significance and Societal Importance of the Research Achievements |
従来起こりにくいと考えられてきた環状RNAの翻訳反応が起こりうること、塩基配列が翻訳効率に与える影響について検証し明らかにした。mRNAを医薬品として用いるに際しその安定性向上や翻訳能向上が必要であり、そのための方法論を多角的に検証した。その結果、今後の核酸医薬品の開発に役立つ知見を得た。
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Report
(4 results)
Research Products
(20 results)
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[Patent(Industrial Property Rights)] 切断プライマー2020
Inventor(s)
阿部洋、阿部奈保子、中本航介、村瀬裕貴、木村康明
Industrial Property Rights Holder
阿部洋、阿部奈保子、中本航介、村瀬裕貴、木村康明
Industrial Property Rights Type
特許
Industrial Property Number
2019-140851
Filing Date
2020
Related Report
-
[Patent(Industrial Property Rights)] 停止プライマー2020
Inventor(s)
阿部洋、阿部奈保子、中本航介、村瀬裕貴、木村康明
Industrial Property Rights Holder
阿部洋、阿部奈保子、中本航介、村瀬裕貴、木村康明
Industrial Property Rights Type
特許
Industrial Property Number
2019-140852
Filing Date
2020
Related Report
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