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New insights into the diazotrophs in terrestrial environments

Research Project

Project/Area Number 18K14366
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 38010:Plant nutrition and soil science-related
Research InstitutionThe University of Tokyo

Principal Investigator

Masuda Yoko  東京大学, 大学院農学生命科学研究科(農学部), 特任研究員 (80813237)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords鉄還元菌 / 窒素固定 / 水田土壌 / メタゲノム / Geomonas / Oryzomonas / Anaeromyxobacter / Geobacter / メタゲノム解析 / 安定同位体 / 土壌
Outline of Final Research Achievements

In our metagenomic analysis of the terrestrial soils around the world, nif genes derived from iron-reducing bacteria were detected in 79 among 127 sites. The nif genes derived from iron-reducing bacteria was frequently detected not only in paddy soils but also in the forest, desert, and polar soils. These results suggested that nitrogen fixation by iron-reducing bacteria is a universal phenomenon in the soil environment.
We verified, for the first time, the nitrogen-fixing activity of Anaeromyxobacter isolated from paddy soil, both in culture medium and soil microcosm. In addition, we successfully isolated several strains of iron-reducing belonging to new genera, Geomonas and Oryzomonas, and confirmed their nitrogen-fixing activity.

Academic Significance and Societal Importance of the Research Achievements

窒素固定は大気中の窒素ガスを生物が利用できる形態の窒素に変換して土壌に供給する重要な反応である。19世紀末にはじめて窒素固定が発見されて以降、「土壌の窒素固定は植物共生細菌や光合成細菌が担う」というのが一般常識であった。しかし本研究により、窒素固定できる鉄還元菌が地球規模で普遍的に分布していること、そして既知の窒素固定細菌群よりも存在量が多い傾向も見出された。このことは窒素固定に関する定説を刷新して自然生態系の支持基盤の本質に迫るものであり、土壌学分野だけでなく、微生物学、地球科学、生態学といった自然科学分野全般において意義を持つ。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (15 results)

All 2021 2020 2019 2018

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 1 results) Presentation (9 results) (of which Int'l Joint Research: 5 results,  Invited: 5 results)

  • [Journal Article] Geomonas silvestris sp. nov., Geomonas paludis sp. nov. and Geomonas limicola sp. nov., isolated from terrestrial environments, and emended description of the genus Geomonas2021

    • Author(s)
      Itoh Hideomi、Xu Zhenxing、Masuda Yoko、Ushijima Natsumi、Hayakawa Chie、Shiratori Yutaka、Senoo Keishi
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology

      Volume: 71 Issue: 1

    • DOI

      10.1099/ijsem.0.004607

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhancement of the nitrogen-fixing activity of paddy soils owing to iron application2021

    • Author(s)
      Masuda Yoko、Shiratori Yutaka、Ohba Hirotomo、Ishida Takanori、Takano Ryo、Satoh Sakura、Shen Weishou、Gao Nan、Itoh Hideomi、Senoo Keishi
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 1 Issue: 3 Pages: 1-5

    • DOI

      10.1080/00380768.2021.1888629

    • NAID

      210000158861

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Description of Three Novel Members in the Family Geobacteraceae, Oryzomonas japonicum gen. nov., sp. nov., Oryzomonas sagensis sp. nov., and Oryzomonas ruber sp. nov.2020

    • Author(s)
      Xu Zhenxing、Masuda Yoko、Hayakawa Chie、Ushijima Natsumi、Kawano Keisuke、Shiratori Yutaka、Senoo Keishi、Itoh Hideomi
    • Journal Title

      Microorganisms

      Volume: 8 Issue: 5 Pages: 634-634

    • DOI

      10.3390/microorganisms8050634

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] DiazotrophicAnaeromyxobacterIsolates from Soils2020

    • Author(s)
      Masuda Yoko、Yamanaka Haruka、Xu Zhen-Xing、Shiratori Yutaka、Aono Toshihiro、Amachi Seigo、Senoo Keishi、Itoh Hideomi
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 86 Issue: 16

    • DOI

      10.1128/aem.00956-20

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 水田土壌における鉄還元菌窒素固定の発見と応用 オミクス解析から低窒素農業へ2020

    • Author(s)
      増田曜子
    • Journal Title

      化学と生物

      Volume: 58 Pages: 143-150

    • NAID

      130007993004

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Geomonas oryzae gen. nov., sp. nov., Geomonas edaphica sp. nov., Geomonas ferrireducens sp. nov., Geomonas terrae sp. nov., four ferric-reducing bacteria isolated from paddy soil, and reclassification of three species of the genus Geobacter as members of the genus Geomonas gen. nov.2019

    • Author(s)
      Zhenxing Xu, Yoko Masuda, Hideomi Itoh, Natsumi Ushijima, Yutaka Shiratori, Keishi Senoo
    • Journal Title

      Frontiers in Microbiology

      Volume: 10 Pages: 2201-2201

    • DOI

      10.3389/fmicb.2019.02201

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 見落とされてきた微生物の生態-メタトランスクリプトーム解析の応用-2021

    • Author(s)
      増田曜子
    • Organizer
      土壌微生物×物質循環ワークショップ
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 水田土壌における鉄還元菌窒素固定の発見と応用 -マイクロバイオーム解析から低窒素農業へ-2019

    • Author(s)
      増田曜子
    • Organizer
      土壌微生物学会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Nitrogen fixation by iron reducing bacteria - previously overlooked diazotrophs essential for sustainable soil-plant ecosystems -2019

    • Author(s)
      Masuda Yoko
    • Organizer
      ASME
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Nitrogen fixation of iron reducing bacteria in rice paddy soils: potent agents for sustainable crop production with low nitrogen input2019

    • Author(s)
      Masuda Yoko
    • Organizer
      BAGECO
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Semi-ubiquitous presence of iron reducing bacterial nif genes in terrestrial soils2018

    • Author(s)
      Yoko Masuda
    • Organizer
      ASME
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 鉄還元細菌の窒素固定能の検証2018

    • Author(s)
      山中遥加
    • Organizer
      日本土壌肥料学会2018年度大会(神奈川大会)
    • Related Report
      2018 Research-status Report
  • [Presentation] 水田土壌の優占種、鉄還元菌の新機能-窒素肥沃度を支えるキープレーヤー-2018

    • Author(s)
      増田曜子
    • Organizer
      第11回北陸合同バイオシンポジウム2018
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Nitrogen fixing activity of iron reducing bacteria in paddy soils: potent agents for low nitrogen rice production in East Asia2018

    • Author(s)
      Yoko Masuda
    • Organizer
      NARO-MARCO
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Iron-reducing bacteria is a ubiquitous but previously-overlooked driver of reductive nitrogen transformation in terrestrial ecosystems2018

    • Author(s)
      Yoko Masuda
    • Organizer
      ASME
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2018-04-23   Modified: 2022-01-27  

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