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Elucidation of the genes involved in Fe excess response mechanism and enhancement of Fe excess tolerance in rice

Research Project

Project/Area Number 18K14367
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 38010:Plant nutrition and soil science-related
Research InstitutionAkita Prefectural University (2019-2020)
Ishikawa Prefectural University (2018)

Principal Investigator

Aung May Sann  秋田県立大学, 生物資源科学部, 特任助教 (20799671)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords鉄過剰 / イネ / OsNAS3 / ニコチアナミン / シス制御因子 / ストレス耐性 / バイオインフォマティクス / マイクロアレイ / Fe excess / Rice / bioinformatics / stress tolerance / microarray
Outline of Final Research Achievements

Iron excess is a major problem in rainfed rice cultivation in acidic soils. I conducted research to elucidate the tolerance mechanism, and obtained the following results. (1) OsNAS3 plays an important role in iron excess tolerance in rice. (2) T-DNA insertion mutant genes whose expressions are highly induced by iron excess were used. They were grown under iron-excess conditions, and the phenotypes were observed, and physiological data were analyzed. The results suggested that target genes play important roles in iron excess tolerance. (3) Using bioinformatics methods, I searched for novel cis-regulatory elements and promoter structures involved in the iron excess response. The results of these studies are expected to contribute to breeding of iron excess-tolerant rice and establishing a cultivation method for rice that avoids iron excess toxicity.

Academic Significance and Societal Importance of the Research Achievements

世界の 30 %の土壌は酸性土壌であり、特に中国や東南アジアあるいはアフリカの天水稲作地でのイネの生産において深刻な問題となっている。鉄過剰によりイネの収量が1割から5割低下し、まったく収穫できないこともある。毎年世界中で、8000万トンの収量が失われ、400億ドルの経済的損失が引き起こされている。本研究成果は、鉄過剰耐性のイネを育種したり、鉄過剰症を回避するイネの栽培方法を確立することで、鉄分過剰が発生しやすい土壌でのイネの生産性を向上することに、非常に役立つと期待さる。本研究成果をもとに、近い将来、遺伝子導入や品種改良などの様々な方法で、新しい鉄耐性イネが誕生することが期待される。

Report

(3 results)
  • 2020 Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (11 results)

All 2020 2019 2018

All Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (6 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Book (1 results)

  • [Journal Article] Synthesis by OsNAS3 Is Important for Mitigating Iron Excess Stress in Rice.2019

    • Author(s)
      Aung MS, Masuda H, Nozoye T,Kobayashi T, Jeon J-S, An G, Nishizawa NK.
    • Journal Title

      Frontiers in Plant Sciences

      Volume: 10 Pages: 660-660

    • DOI

      10.3389/fpls.2019.00660

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Enhancement of iron acquisition in rice by the mugineic acid synthase gene with ferric iron reductase gene and OsIRO2 confers tolerance in submerged and non-submerged calcareous soils.2019

    • Author(s)
      Masuda H, Aung MS, Kobayashi T, Hamada T, Nishizawa NK.
    • Journal Title

      Frontiers in Plant Sciences

      Volume: 10 Pages: 1179-1179

    • DOI

      10.3389/fpls.2019.01179

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Iron-deficiency response and expression of genes related to iron homeostasis in poplars2018

    • Author(s)
      3.Hiroshi Masuda, May Sann Aung, Keisuke Maeda, Takanori Kobayashi, Naoki Takata, Toru Taniguchi, Naoko Kishi Nishizawa
    • Journal Title

      Journal of Soil Science and Plant Nutrition

      Volume: 64 Issue: 5 Pages: 576-588

    • DOI

      10.1080/00380768.2018.1480325

    • NAID

      210000012015

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] イネの鉄過剰:生理的応答と分子メカニズム2018

    • Author(s)
      May Sann Aung, 増田寛志
    • Journal Title

      アグリバイオ

      Volume: 2 Pages: 91-92

    • Related Report
      2018 Research-status Report
  • [Presentation] Iron biofortification of rice by introduction of GmFerritin, HvNAS1, OsYSL2 and mugeinic acid synthase gene (IDS3) with rice OsALS as a marker gene2019

    • Author(s)
      May Sann Aung, Hiroshi Masuda, Takanori Kobayashi, Naoko K. Nishizawa
    • Organizer
      第65回 日本土壌肥料学会 全国大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Nicotianamine synthase, OsNAS3, mitigates iron excess in rice2019

    • Author(s)
      May Sann Aung, Hiroshi Masuda, Takanori Kobayashi, Naoko K. Nishizawa
    • Organizer
      17th International Symposium on Rice Functional Genomics
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Nicotianamine synthesis by OsNAS3 is important for mitigation of iron excess stress in rice2018

    • Author(s)
      May Sann Aung, Hiroshi Masuda, Tomoko Nozoye, Takanori Kobayashi, Gynheung An, Jong-Seong Jeon, Naoko K. Nishizawa
    • Organizer
      19th International Symposium on Iron Nutrition and Interaction in Plants (ISINIP) (Taipei, Taiwan)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Nicotianamine synthesis by OsNAS3 is important for iron excess tolerance in rice2018

    • Author(s)
      May Sann Aung, Hiroshi Masuda, Tomoko Nozoye, Takanori Kobayashi, Naoko K. Nishizawa
    • Organizer
      第64回 土壌肥料学会神奈川大会
    • Related Report
      2018 Research-status Report
  • [Presentation] イネの鉄過剰症と鉄過剰応答に関わる分子メカニズム2018

    • Author(s)
      May Sann Aung
    • Organizer
      第42回 日本鉄バイオサイエンス学会 金沢大会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Iron toxicity in rice: Physiological and transcriptomic analysis in various rice tissues2018

    • Author(s)
      May Sann Aung, Hiroshi Masuda, Takanori Kobayashi, Naoko K. Nishizawa
    • Organizer
      16th International Symposium on Rice Functional Genomics (Tokyo)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Book] Iron Biofortification: The Gateway to Overcoming Hidden Hunger. In: Costa de Oliveira A., Pegoraro C., Ebeling Viana V. (eds) The Future of Rice Demand: Quality Beyond Productivity.2020

    • Author(s)
      May Sann Aung, Hiroshi Masuda, Takanori Kobayashi, Naoko K. Nishizawa.
    • Publisher
      Springer
    • ISBN
      9783030375096
    • Related Report
      2019 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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