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Screening of genes useful for genetic engineering of carbon dioxide fixation

Research Project

Project/Area Number 18K14375
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 38020:Applied microbiology-related
Research InstitutionResearch Institute of Innovative Technology for the Earth (2019)
Kobe University (2018)

Principal Investigator

Kobayashi Jyumpei  公益財団法人地球環境産業技術研究機構, その他部局等, 研究員 (90759987)

Project Period (FY) 2018-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords光合成細菌 / 二酸化炭素固定 / CO2固定 / Rhodobacter sphaeroides / 紅色非硫黄細菌
Outline of Final Research Achievements

To elucidate effect of genes presumed to be involved in carbon dioxide fixation on photosynthetic bacteria, 14 genes were selected from sequence information. Fourteen strains that alone or multiply overexpress these genes were constructed and grown in medium containing sodium bicarbonate as a sole carbon source. As a result, the 6 stains showed improved growth rate and bicarbonate consumption compared to the parent strain among 14 recombinant strains. From these results, the 6 useful genes for carbon dioxide fixation were revealed.

Academic Significance and Societal Importance of the Research Achievements

エネルギー源として化石燃料を中心に利用している現在の社会において、化石燃料の燃焼によって大気に放出される二酸化炭素を主原因とした地球温暖化は重大な懸念事項である。そのため、遺伝子組み換えによって微生物による二酸化炭素の利用能力を向上させることは、単純な二酸化炭素の削減にとどまらず、二酸化炭素から直接有用な物質を生産するために重要である。本研究によって6つの遺伝子が光合成細菌の二酸化炭素利用に応用できる可能性が明らかになった。この成果は持続可能な社会の実現の一助となりうると考えられる。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (3 results)

All 2019

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results)

  • [Journal Article] Complete Genome Sequence of Rhodobacter sphaeroides Strain HJ, a Purple Nonsulfur Bacterium with the Ability To Produce High Levels of Hydrogen from Acetate2019

    • Author(s)
      Kobayashi Jyumpei、Kondo Akihiko
    • Journal Title

      Microbiology Resource Announcements

      Volume: 8 Issue: 37

    • DOI

      10.1128/mra.00652-19

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] d-Amino Acids and Lactic Acid Bacteria2019

    • Author(s)
      Kobayashi Jyumpei
    • Journal Title

      Microorganisms

      Volume: 7 Issue: 12 Pages: 690-690

    • DOI

      10.3390/microorganisms7120690

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Disruption of poly (3-hydroxyalkanoate) depolymerase gene and overexpression of three poly (3-hydroxybutyrate) biosynthetic genes improve poly (3-hydroxybutyrate) production from nitrogen rich medium by Rhodobacter sphaeroides.2019

    • Author(s)
      Kobayashi J, Kondo A
    • Journal Title

      Microbial Cell Factries

      Volume: 18 Issue: 1 Pages: 40-40

    • DOI

      10.1186/s12934-019-1088-y

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access

URL: 

Published: 2018-04-23   Modified: 2021-02-19  

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