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Creation of Bio-photocatalyst Using Microorganism with Artificial Light-driven Coenzyme Regeneration System

Research Project

Project/Area Number 18K14376
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 38020:Applied microbiology-related
Research InstitutionNara Women's University

Principal Investigator

Honda Yuki  奈良女子大学, 自然科学系, 助教 (90583849)

Project Period (FY) 2018-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords光触媒 / 生体触媒 / 水素生産 / 補酵素再生 / ポルフィリン
Outline of Final Research Achievements

Biocatalysts, including enzymes or microorganisms, are capable of catalyzing highly selective and efficient reactions under environmentally friendly condition. This feature of biocatalysis would contribute to developing an energy-saving process in industry. In an industrial application of biocatalysis, supplying energy to proceed a certain reaction has been a problem in the enzymatic reaction which requires a coenzyme, such as NADH. This study addressed this problem by developing a light-driven biocatalysis system which consists of the combination of photosensitizer and enzyme. In such system, photosensitizer serves energy to enzyme from light energy, instead of costly coenzyme supplementation. This study achieved a large-scale synthesis of porphyrin dye by genetically engineered bacteria, the photoreduction of an artificial coenzyme (MV) by the dye, and one of the light-driven biocatalysis by coupling the photoreduction of MV and the hydrogen-forming enzyme.

Academic Significance and Societal Importance of the Research Achievements

酵素反応の利用において、反応の進行に必要な補酵素の再生は重要な課題であった。グルコース脱水素酵素との共役反応などは古くから用いられる補酵素再生系であり、また種々の方法が考案されている。本研究では、安価に入手できる光によって、かつ生体分子によって酵素へのエネルギー供給を実現する新規な反応系を構築し、新たな方法論による補酵素再生の実現を目指した。補酵素再生という課題に対して、従来とは異なる方法論で解決を目指す点で学術的な意義があり、かつ、その効果は生体触媒の工業的利用による省エネルギープロセスの実現に寄与することが期待されるころから、社会的な意義のある研究提案であった。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (6 results)

All 2020 2018

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (3 results)

  • [Journal Article] 光エネルギーによって駆動する生体触媒反応2020

    • Author(s)
      本田裕樹
    • Journal Title

      生物工学会誌「バイオミディア」

      Volume: 98 Pages: 81-81

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 無機ー生体ハイブリッド型光触媒反応:無機半導体とヒドロゲナーゼを組み合わせた光駆動型の水素生産2020

    • Author(s)
      本田裕樹
    • Journal Title

      酵素工学ニュース

      Volume: 83 Pages: 19-24

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Coexpression of 5-Aminolevulinic Acid Synthase Gene Facilitates Heterologous Production of Thermostable Cytochrome P450, CYP119, in Holo Form in Escherichia coli2018

    • Author(s)
      Honda Yuki、Nanasawa Kii、Fujii Hiroshi
    • Journal Title

      ChemBioChem

      Volume: 19 Issue: 20 Pages: 2156-2159

    • DOI

      10.1002/cbic.201800331

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] Light-driven and mediator-free hydrogen evolution using a combination of a photosensitizer and a recombinant <i>Escherichia coli</i> whole-cell biocatalyst2020

    • Author(s)
      Yuki Honda, Yuka Shinohara, Hiroshi Fujii
    • Organizer
      日本化学会第100回春季年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 光増感剤/組換え大腸菌細胞系を用いた光触媒的水素生産2020

    • Author(s)
      本田裕樹、篠原優佳、藤井浩
    • Organizer
      日本農芸化学会2020年度大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 微生物の金属硫化物生成能と遺伝子工学的な水素生成能付与を組み合わせた光駆動型水素生成2020

    • Author(s)
      篠原優佳、本田裕樹、渡邊源規、石原達己、藤井浩
    • Organizer
      日本農芸化学会2020年度大会
    • Related Report
      2019 Annual Research Report

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Published: 2018-04-23   Modified: 2021-02-19  

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