Sugar utilization strategy of bifidobacteria that supports the symbiosis with their hosts

• Project/Area Number: 18K14379
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 38020:Applied microbiology-related
• Research Institution: Kyoto University (2020); Ishikawa Prefectural University (2018-2019)
• Principal Investigator: Sakanaka Mikiyasu 京都大学, 生命科学研究科, 特定准教授 (60801892)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: ビフィズス菌 / 母乳オリゴ糖 / トランスポーター / 遺伝子操作 / 共生 / 遺伝子破壊

Outline of Final Research Achievements

Bifidobacteria are predominant in the guts of breastfed infants, which is mainly attributed to the ability of bifidobacteria to utilize oligosaccharides contained in breast milk (human milk oligosaccharides; HMOs). However, the molecular mechanisms of the HMO uptake remain unclear. Our study identified new HMO transporters from bifidobacteria, which expanded our understanding of the symbiotic mechanism between bifidobacteria and infants mediated by HMOs.

Academic Significance and Societal Importance of the Research Achievements

近年、乳児期に形成される腸内細菌叢は、乳児期のみならず生涯にわたりヒトの健康に関与していることが示されている。したがって、有益な乳児腸内細菌叢である「ビフィズス菌優勢な細菌叢」が如何にして形成されるかを理解することは非常に重要である。本研究では、ビフィズス菌の乳児腸内での増殖機構の一端を解明することができた。本研究で得た知見は、ビフィズス菌が優勢な細菌叢の形成手法を今後確立するのに役立つと考えられる。

Research Products

• [Int'l Joint Research] Technical University of Denmark(デンマーク)
• [Int'l Joint Research] Technical University of Denmark(デンマーク)
• [Journal Article] Enzymatic Adaptation of Bifidobacterium bifidum to Host Glycans, Viewed from Glycoside Hydrolyases and Carbohydrate-Binding Modules (2020)
  • Author(s): Katoh Toshihiko、Ojima Miriam N.、Sakanaka Mikiyasu、Ashida Hisashi、Gotoh Aina、Katayama Takane
  • Journal Title: Microorganisms Volume: 8 Issue: 4 Pages: 481-481
  • DOI: 10.3390/microorganisms8040481
  • Peer Reviewed / Open Access
• [Journal Article] Evolutionary adaptation in fucosyllactose uptake systems supports bifidobacteria-infant symbiosis (2019)
  • Author(s): M. Sakanaka, M.E. Hansen, A. Gotoh, T. Katoh, K. Yoshida, T. Odamaki, H. Yachi, Y. Sugiyama, S. Kurihara, J. Hirose, T. Urashima, J. Xiao, M. Kitaoka, S. Fukiya, A. Yokota, L.L. Leggio, M.A. Hachem, T. Katayama
  • Journal Title: Science Advance Volume: 5 Issue: 8
  • DOI: 10.1126/sciadv.aaw7696
  • NAID: 120006716596
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Varied Pathways of Infant Gut-Associated Bifidobacterium to Assimilate Human Milk Oligosaccharides: Prevalence of the Gene Set and Its Correlation with Bifidobacteria-Rich Microbiota Formation (2019)
  • Author(s): Sakanaka Mikiyasu、Gotoh Aina、Yoshida Keisuke、Odamaki Toshitaka、Koguchi Hiroka、Xiao Jin-zhong、Kitaoka Motomitsu、Katayama Takane
  • Journal Title: Nutrients Volume: 12 Issue: 1 Pages: 71-71
  • DOI: 10.3390/nu12010071
  • NAID: 120006784431
  • Peer Reviewed / Open Access
• [Journal Article] A transposon mutagenesis system for Bifidobacterium longum subsp. longum based on an IS3 family insertion sequence, ISBlo11 (2018)
  • Author(s): Sakanaka Mikiyasu、Nakakawaji Shingo、Nakajima Shin、Fukiya Satoru、Abe Arisa、Saburi Wataru、Mori Haruhide、Yokota Atsushi
  • Journal Title: Applied and Environmental Microbiology Volume: 84 Issue: 17
  • DOI: 10.1128/aem.00824-18
  • Peer Reviewed
• [Journal Article] Sharing of human milk oligosaccharides degradants within bifidobacterial communities in faecal cultures supplemented with Bifidobacterium bifidum (2018)
  • Author(s): Gotoh Aina、Katoh Toshihiko、Sakanaka Mikiyasu、Ling Yiwei、Yamada Chihaya、Asakuma Sadaki、Urashima Tadasu、Tomabechi Yusuke、Katayama-Ikegami Ayako、Kurihara Shin、Yamamoto Kenji、Harata Gaku、He Fang、Hirose Junko、Kitaoka Motomitsu、Okuda Shujiro、Katayama Takane
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 13958-13958
  • DOI: 10.1038/s41598-018-32080-3
  • NAID: 120006817605
  • Peer Reviewed / Open Access
• [Presentation] 人乳成分の代謝から考えるビフィズス菌の適応進化戦略 (2021)
  • Author(s): 阪中幹祥
  • Organizer: ビフィズス菌研究会設立記念第一回シンポジウム
  • Invited
• [Presentation] 母乳オリゴ糖トランスポーターの機能解析から見出されるビフィズス菌の適応進化戦略 (2020)
  • Author(s): 阪中幹祥
  • Organizer: 第43回日本分子生物学会年会
  • Invited
• [Presentation] Evolutionary adaptation occurred in human milk oligosaccharide transporters supports symbiosis between breast-fed infants and bifidobacteria (2019)
  • Author(s): Sakanaka Mikiyasu, Hansen Morten Ejby, Gotoh Aina, Katoh Toshihiko, Yoshida Keisuke, Odamaki Toshitaka, Yachi Hiroyuki, Sugiyama Yuta, Kurihara Shin, Hirose Junko, Urashima Tadasu, Xiao Jin-zhong, Kitaoka Motomitsu, Fukiya Satoru, Yokota Atsushi, Lo Leggio Leila, Abou Hachem Maher, Katayama Takane
  • Organizer: Keystone Symposia, Microbiome: Therapeutic Implications (T1)
  • Int'l Joint Research
• [Presentation] ビフィズス菌のトランスポゾン変異株を用いたα-グルコシダーゼ遺伝子の機能解明 (2019)
  • Author(s): 阪中 幹祥、中川路 伸吾、中島 森、阿部 光紗、佐分利 亘、森 春英、横田 篤、吹谷 智
  • Organizer: 日本農芸化学会2019年度大会
• [Presentation] Transposon mutagenesis in Bifidobacterium longum subsp. longum and characterization of a gene involved in carbohydrate assimilation (2018)
  • Author(s): Mikiyasu Sakanaka、Shingo Nakakawaji、Shin Nakajima、Satoru Fukiya、Arisa Abe、Wataru Saburi、Haruhide Mori、Atsushi Yokota
  • Organizer: The Danish Microbiological Society Annual Congress 2018
  • Int'l Joint Research
• [Presentation] A transposon mutagenesis system in Bifidobacterium longum subsp. longum and functional analysis of an oligosaccharide-assimilation gene (2018)
  • Author(s): Mikiyasu Sakanaka、Shingo Nakakawaji、Shin Nakajima、Arisa Abe、Wataru Saburi、Haruhide Mori、Atsushi Yokota、Satoru Fukiya
  • Organizer: The 7th Beneficial Microbes Conference
  • Int'l Joint Research