Microscopic analysis of degenerated fungal culture in edible mushrooms
Project/Area Number |
18K14382
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 38020:Applied microbiology-related
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Research Institution | Hokkaido Research Organization |
Principal Investigator |
Saitoh Sayaka 地方独立行政法人北海道立総合研究機構, 森林研究本部 林産試験場, 研究職員 (30804299)
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Project Period (FY) |
2018-04-01 – 2021-03-31
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Project Status |
Completed (Fiscal Year 2020)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2019: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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Keywords | 食用きのこ / 種菌劣化 / 蛍光免疫染色 / イメージング解析 / 蛍光イメージング / イメージング / 酸化ストレス / きのこ / 菌糸 / 細胞生物学 |
Outline of Final Research Achievements |
The Japanese mushroom industry plays essential roles, from food production to pharmaceutical ingredients, which breeding strains especially contribute to these developments. However, culture degeneration during subsequent culture maintenance occurs in commercial mushroom strains spontaneously, that triggers reduction of fruiting ability and is a longstanding problem. Here, I focused on microscopic properties of cellular organelles in degenerated strains. Comparative analysis between high-fruiting and low-fruiting strains in Grifola frondosa, Pleurotus cornucopiae var. citrinopileatus, and Flammulina velutipes revealed that staining pattern of cytoskeleton filaments and mitochondria varied in low-fruiting strains, suggesting that degenerated strains have alteration in cellular organelles before spawn-inoculation for cultivation.
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Academic Significance and Societal Importance of the Research Achievements |
種菌劣化については、その要因および機序、そして菌種間における種菌劣化の共通点・相違点は明らかになっていない。一方できのこ産業においては食用きのこが半工業的に大量生産されていることから、きのこ種菌の収量性の劣化は収益性に大きく影響する。顕微鏡イメージングによる菌糸の解析は食用きのこでは報告例が少ないことから、細胞生物学の面から劣化種菌細胞に起きている異常と子実体形成への関連を検証する本研究は、学術面およびきのこ産業の社会面双方において意義ある研究といえる。
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Report
(3 results)
Research Products
(2 results)