Metabolomic study of molecular mechanisms of oxalate accumulation for quality improvement of rice straw
| Project/Area Number |
18K14386
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 38030:Applied biochemistry-related
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| Research Institution | Yamagata University (2021-2022)
Saitama University (2018-2020) |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | シュウ酸 / イネ / メタボローム解析 / 正逆染色体部分置換系統 / 品種間比較 / イソクエン酸リアーゼ / 重イオンビーム / CE-MS / 世界のイネ・コアコレクション / 日本のイネ・コアコレクション / CE-QQQ-MS / ICL / メタボローム / 染色体部分置換系統 / メタボロミクス |
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| Outline of Final Research Achievements |
This research project aimed to elucidate mechanisms of oxalate accumulation at the genetic level in order to reduce the oxalate content of rice leaves for improvement the quality of rice straw. The results showed that isocitrate lyase, a key enzyme in the isocitrate pathway, one of the oxalate synthesis pathways, would contribute to oxalate accumulation during the dark period. In addition, the analysis of chromosomal partial substitution lines of Koshihikari and Takanari revealed three candidate genes that may affect oxalate content. Furthermore, metabolome analysis of rice brittle culm mutants revealed that some of the genes involves in the cell wall composition may also affect oxalate content.
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| Academic Significance and Societal Importance of the Research Achievements |
シュウ酸はヒトや家畜のミネラル不足や腎結石を引き起こす劇物であるため、作物におけるシュウ酸の低減は農業上の重要課題である。本研究課題において、シュウ酸蓄積に影響を及ぼすとされる因子として、これまで研究代表者が着目していたICL遺伝子に加え、本研究課題によって新たに複数の候補遺伝子が見出された。今後、これらの遺伝子の解析を進めることにより、稲わらの高品質化やホウレンソウなどの高シュウ酸作物の品種改良に役立つことが、ひいてはヒトや家畜の疾患予防につながることが期待できる。
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Report
(6 results)
Research Products
(71 results)
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[Journal Article] Loss of peroxisomal NAD kinase 3 (NADK3) affects photorespiration metabolism in Arabidopsis2023
Author(s)
Shota Suzuki, Daimu Tanaka, Atsuko Miyagi, Kentaro Takahara, Masaru Kono, Chaomurilege, Ko Noguchi, Toshiki Ishikawa, Minoru Nagano, Masatoshi Yamaguchi, Maki Kawai-Yamada
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Journal Title
Journal of Plant Physiology
Volume: 283
Pages: 153950-153950
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Phosphorus toxicity disrupts Rubisco activation and reactive oxygen species defence systems by phytic acid accumulation in leaves2020
Author(s)
Takagi D, Miyagi A, Tazoe Y, Suganami M, Kawai-Yamada M, Ueda A, Suzuki Y, Noguchi K, Hirotsu N, Makino A
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Journal Title
Plant, Cell and Environment
Volume: －
Issue: 9
Pages: 2033-2053
DOI
Related Report
Peer Reviewed / Open Access
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