Understanding the mechanism of post-transcriptional regulation in plant mitochondria

• Project/Area Number: 18K14435
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 38060:Applied molecular and cellular biology-related
• Research Institution: Kyushu University (2020); Nagoya University (2018-2019)
• Principal Investigator: Ichinose Mizuho 九州大学, 農学研究院, 学術研究員 (60755718)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 植物 / オルガネラ / ミトコンドリア / 葉緑体 / PPRタンパク質 / RNA制御 / 遺伝子発現 / 転写後制御 / RNA結合タンパク質 / 転写後調節 / 遺伝子発現制御 / RNAスプライシング / PPR / RNA安定化 / RNA編集 / クロストーク

Outline of Final Research Achievements

Plant mitochondria is an important organelle for plant growth and development. Mitochondria has own gene expression system and this is tightly regulated at post-transcriptional level. However, the mechanism of post-transcriptional regulation is still unknown. In this study, we identified new factors involved in organelle RNA regulation such as splicing and RNA stability.

Academic Significance and Societal Importance of the Research Achievements

ミトコンドリアの正常な機能発現は植物の生育に必須であるにも関わらず、その遺伝子発現機構は未だ不明な点が多い。ミトコンドリア遺伝子発現に働く因子を明らかにすることは、ミトコンドリアの複雑な遺伝子発現システムの理解につながるため、学術的意義は大きい。また、ミトコンドリアの遺伝子発現システムが解明されれば、ミトコンドリア機能増強による有用作物の作出が期待される。

Research Products

• [Journal Article] Moss PPR-SMR protein PpPPR_64 influences the expression of a psaA-psaB-rps14 gene cluster and processing of the 23S-4.5S rRNA precursor in chloroplasts. (2021)
  • Author(s): Ayumu Takahashi, Chieko Sugita, Mizuho Ichinose, Mamoru Sugita
  • Journal Title: Plant Molecular Biology Volume: in press Issue: 4-5 Pages: 417-429
  • DOI: 10.1007/s11103-020-01090-z
  • Peer Reviewed
• [Journal Article] Substitutional RNA Editing in Plant Organelles (2020)
  • Author(s): Mizuho Ichinose, Mamoru Sugita
  • Journal Title: Methods in Molecular Biology Volume: 2181 Pages: 1-12
  • DOI: 10.1007/978-1-0716-0787-9_1
  • ISBN: 9781071607862, 9781071607879
  • Peer Reviewed
• [Journal Article] The L motifs of two moss pentatricopeptide repeat proteins are involved in RNA editing but predominantly not in RNA recognition (2020)
  • Author(s): Matsuda Takuya、Sugita Mamoru、Ichinose Mizuho
  • Journal Title: PLOS ONE Volume: 15 Issue: 4 Pages: e0232366-e0232366
  • DOI: 10.1371/journal.pone.0232366
  • Peer Reviewed / Open Access
• [Journal Article] Two novel PLS-class pentatricopeptide repeat proteins are involved in the group II intron splicing of mitochondrial transcripts in the moss Physcomitrella patens (2020)
  • Author(s): Ichinose, M., Ishimaru, A., Sugita, C., Nakajima, K., Kawaguchi, Y., Sugita, M.
  • Journal Title: Plant and Cell Physiology Volume: -
  • Peer Reviewed
• [Journal Article] The P-class pentatricopeptide repeat protein PpPPR_21 is needed for accumulation of the psbI-ycf12 dicistronic mRNA in Physcomitrella chloroplasts. (2019)
  • Author(s): Ebihara, T., Matsuda, T., Sugita, C., Ichinose, M., Yamamoto, H., Shikanai, T., Sugita, M.
  • Journal Title: The Plant Journal Volume: 97 Issue: 6 Pages: 1120-1131
  • DOI: 10.1111/tpj.14187
  • NAID: 120006650153
  • Peer Reviewed
• [Journal Article] The DYW domains of pentatricopeptide repeat protein RNA editing factors contribute to discrimination of target and non-target editing sites. (2018)
  • Author(s): Ichinose, M. and Sugita, M.
  • Journal Title: Plant & Cell Physiol. Volume: 59 Issue: 8 Pages: 1652-1659
  • DOI: 10.1093/pcp/pcy086
  • Peer Reviewed
• [Presentation] ヒメツリガネゴケのミトコンドリアP-class PPRタンパク質の機能解析 (2019)
  • Author(s): 一瀬瑞穂、杉田 護
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] 葉緑体ndh遺伝子の転写後制御に関わるPPRタンパク質 (2019)
  • Author(s): 伊藤綾花、杉田千恵子、一瀬瑞穂、加藤義宣、山本 宏、鹿内利治、杉田 護
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] Pクラス・ペンタトリコペプチドタンパク質は葉緑体psbI-ycf12 mRNAの蓄積に必要である (2019)
  • Author(s): 海老原哲男、松田拓也、杉田千恵子、一瀬瑞穂、山本 宏、鹿内利治、杉田 護
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] PPR proteins as post-transcriptional regulators in plant organelles (2018)
  • Author(s): Ichinose, M., Sugita, C., Ishimaru, A., Ito, A. Ebihara, T. and Sugita, M.
  • Organizer: International Symposium on Photosynthesis and Chloroplast Biogenesis 2018
  • Int'l Joint Research