Genetic analysis for photoperiod-depending seed set and maturity time which defines the ecotype of common buckwheat (Fagopyrum esculentum)

• Project/Area Number: 18K14448
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 39010:Science in plant genetics and breeding-related
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: Takeshima Ryoma 国立研究開発法人農業・食品産業技術総合研究機構, 次世代作物開発研究センター, 研究員 (70806603)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: AmpliSeq / GRAS-Di / QTL / ソバ / 登熟期 / 日長反応 / 感光性 / 日長反応性 / 普通ソバ / 遺伝解析 / 結実

Outline of Final Research Achievements

Common buckwheat is classified into summer, intermediate, and autumn ecotypes based on their photoperiod responsiveness. This study aims to detect the genetic loci for photoperiod-depending seed setting and maturity time, which defines the ecotypes. QTL analysis of “summer×mid-autumn” crosses detected QTLs for summerization, which results in early seed setting and maturation. The QTLs for flowering time and maturity time were also detected at different loci, indicating that there may be different regulatory mechanisms.

Academic Significance and Societal Importance of the Research Achievements

生態型を制御する遺伝子を明らかにし、育種に利用可能なマーカーを開発できれば、高品質な秋型品種の夏型化による春まき栽培地域の増加や、低緯度地域に向けた秋型品種の晩生化による多収化栽培により、ソバの反収は一気に増加する。これに加えて、高品質な夏型品種は、ソバの需要が高まる夏に新ソバを提供できるため、高付加価値の国産ブランドソバとしての売出しも期待できる。

Research Products

• [Journal Article] Targeted amplicon sequencing + next-generation sequencing?based bulked segregant analysis identified genetic loci associated with preharvest sprouting tolerance in common buckwheat (Fagopyrum esculentum) (2021)
  • Author(s): Takeshima Ryoma、Ogiso-Tanaka Eri、Yasui Yasuo、Matsui Katsuhiro
  • Journal Title: BMC Plant Biology Volume: 21 Issue: 1 Pages: 18-18
  • DOI: 10.1186/s12870-020-02790-w
  • Peer Reviewed / Open Access
• [Presentation] Identification of Genetic Loci Associated with Self-Compatibility, Pre-Harvest Sprouting and Maturity Time Using Targeted Next-Generation Sequencing in Common Buckwheat (Fagopyrum esculentum) (2020)
  • Author(s): Ryoma Takeshima, Eri Ogiso-Tanaka, Yasuo Yasui, Katsuhiro Matsui
  • Organizer: Plant & Animal Genome Conference XXVIII
  • Int'l Joint Research
• [Presentation] 普通ソバの生態型を制御する日長応答性に関わる遺伝解析 (2019)
  • Author(s): 竹島亮馬・小木曽映里・松井勝弘
  • Organizer: 日本育種学会第135回講演会
• [Presentation] An efficient genetic analysis of common buckwheat by using Next-generation sequencing (2018)
  • Author(s): Ryoma Takeshima, Eri Ogiso-Tanaka, Yasuo Yasui, Katsuhiro Matsui
  • Organizer: The 9th Crop Science Seminar in East Asia
  • Int'l Joint Research