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Identification of genes and their functions involved in post-zygotic hybrid inviability in Japanese flowering cherries

Research Project

Project/Area Number 18K14489
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 40010:Forest science-related
Research InstitutionForest Research and Management Organization (2020-2021)
The University of Tokyo (2018-2019)

Principal Investigator

TSURUTA Momi  国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (90809740)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2020: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords雑種不和合性 / サクラ属 / 詳細マッピング / トランスクリプトーム / 植物防御反応 / 自己免疫反応 / 染井吉野 / 防御反応 / 自己免疫 / 生育不全 / RNA-seq / 発現変動遺伝子 / 植物防御反応遺伝子 / 連鎖解析 / 候補遺伝子 / ALOG domain / 発現解析 / 実生の発達
Outline of Final Research Achievements

To understand post-zygotic hybrid incompatibility (HI), we explored genes associated with the lethal growth failure of hybrid cherry seedlings and inferred the mechanism of weak growth by comprehensive expressed gene analysis. The fine-mapping approach allowed us to determine the genomic regions involved in HI and candidate genes for HI. Furthermore, genes that are specifically up or down-regulated in the seedlings were identified. Finally, we concluded that the growth failure of seedlings was caused by the high expression of genes associated with plant defense responses to pathogens.

Academic Significance and Societal Importance of the Research Achievements

種分化は最も根本的な生物学的な興味の一つである。本課題の成果は、「近縁種の分布がしばしば重なり、受精前の生殖隔離機構を持たないサクラ属などの植物種では、なぜ種分化が維持されるのだろうか」との問いに対して、受精後に起こる雑種不和合性の重要性とそれを引き起こすメカニズムの一端を示すことができた。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (9 results)

All 2022 2021 2020 2019

All Journal Article (4 results) (of which Peer Reviewed: 2 results,  Open Access: 4 results) Presentation (5 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Upregulation of defense-related gene expressions associated with lethal growth failure in the hybrid seedlings of Japanese flowering cherry2022

    • Author(s)
      Tsuruta Momi, Lian Chunlan, Mukai Yuzuru
    • Journal Title

      Tree Genetics & Genomes

      Volume: 18 Issue: 3 Pages: 21-21

    • DOI

      10.1007/s11295-022-01552-z

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Hybrid breakdown as a result of up-regulated expression of defense-related genes in the hybrid seedlings of Japanese flowering cherry2021

    • Author(s)
      Tsuruta Momi, Lian Chunlan, Mukai Yuzuru
    • Journal Title

      Proceedings of International Symposium on a Recent Progress in Forest Ecology and Management 2021

      Volume: - Pages: 47-53

    • Related Report
      2021 Annual Research Report
    • Open Access
  • [Journal Article] サクラ属における遺伝基盤の整備と雑種不和合性関連遺伝子の探索2020

    • Author(s)
      鶴田 燃海
    • Journal Title

      Forest Genetics and Tree Breeding

      Volume: 9 Issue: 2 Pages: 65-69

    • DOI

      10.32135/fgtb.9.2_65

    • NAID

      130007836540

    • ISSN
      2187-3453, 2187-350X
    • Year and Date
      2020-04-25
    • Related Report
      2019 Research-status Report
    • Open Access
  • [Journal Article] Fine mapping of a locus presumably involved in hybrid inviability (<i>HIs-1</i>) between flowering cherry cultivar <i>Cerasus</i> × <i>yedoensis</i> ‘Somei-yoshino’ and its wild relative <i>C. spachiana</i>2019

    • Author(s)
      Tsuruta Momi、Mukai Yuzuru
    • Journal Title

      Breeding Science

      Volume: 69 Issue: 4 Pages: 658-664

    • DOI

      10.1270/jsbbs.19078

    • NAID

      130007772613

    • ISSN
      1344-7610, 1347-3735
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Hybrid breakdown as a result of up-regulated expression of defense-related genes in the hybrid seedlings of Japanese flowering cherry2021

    • Author(s)
      Tsuruta Momi, Lian Chunlan, Mukai Yuzuru
    • Organizer
      International Symposium on a Recent Progress in Forest Ecology and Management 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] サクラの生育不全実生で見られた防御関連遺伝子の高発現2021

    • Author(s)
      鶴田燃海・練春蘭・向井譲
    • Organizer
      第132回日本森林学会大会(2021年3月:オンライン)
    • Related Report
      2020 Research-status Report
  • [Presentation] RNA-seq を用いたサクラ属の生育不全実生で特異的に発現する遺伝子の探索2020

    • Author(s)
      鶴田燃海・向井譲・練春蘭
    • Organizer
      131回日本森林学会大会(2020年3月:名古屋)
    • Related Report
      2019 Research-status Report
  • [Presentation] サクラ属における組織分化の制御に関与するALOG gene familyの配列比較2019

    • Author(s)
      鶴田燃海・桐原奏子・向井譲
    • Organizer
      130回日本森林学会大会(2019年3月:新潟)
    • Related Report
      2018 Research-status Report
  • [Presentation] サクラ属における雑種実生の生育不全候補遺伝子の発現特性と塩基配列の変異2019

    • Author(s)
      桐原奏子・向井譲・片畑伸一郎・鶴田燃海
    • Organizer
      130回日本森林学会大会(2019年3月:新潟)
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2023-01-30  

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