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Delimiting species in marine zooplankton using a molecular appriach

Research Project

Project/Area Number 18K14519
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 40040:Aquatic life science-related
Research InstitutionThe University of Tokyo

Principal Investigator

Hirai Junya  東京大学, 大気海洋研究所, 助教 (30762554)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Discontinued (Fiscal Year 2020)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords動物プランクトン / 種多様性 / 一塩基多型 / カイアシ類 / 交雑 / 種分化 / 海洋多様性
Outline of Final Research Achievements

Species complexes with multiple genetic clades are frequently observed using mitochondrial DNA (mtDNA) markers in marine zooplankton. However, reproductive isolation among clades is not fully understood because of low sequence variations in common nuclear markers. In this study, I obtained genome-wide single nucleotide polymorphisms (SNPs) for delimiting species in marine zooplankton with multiple mtDNA clades. For marine copepod Pleuromamma abdominalis with sympatric mtDNA clades, SNPs were obtained by two approaches of MIG-seq and RAD-seq. Both approaches clearly revealed mtDNA clades with reproductive isolations, supporting high cryptic diversity in P. abdominalis. Although both MIG-seq and RAD-seq are useful for delimiting species, MIG-seq uses PCR-based approaches, which can be effective even for small zooplankton with low DNA amounts. Thus, MIG-seq can lead to appropriate measurements of species diversity in the ocean for small zooplankton in the ocean.

Academic Significance and Societal Importance of the Research Achievements

動物プランクトンは種ごとに異なる環境適応能力を有し、分布域の変化などの環境変化に対する応答も種毎で異なる。そのため、動物プランクトンの種の境界を適切に把握することは、生態系の変化を理解する上で重要である。従来の遺伝子解析は母性遺伝をするミトコンドリアDNAの解析が一般的であり、正確な種の境界の把握は困難であった。一方、本研究は次世代シーケンサーを使用し、ゲノム内の一塩基多型を網羅的に取得し、より正確に動物プラントンの種の境界が把握可能であることを示した。この成果は動物プランクトンの分類学に貢献するだけでなく、今後の生物モニタリングにも有用な情報を提供できると考えられる。

Report

(3 results)
  • 2020 Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Research-status Report
  • Research Products

    (14 results)

All 2020 2018

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (12 results) (of which Int'l Joint Research: 8 results)

  • [Journal Article] Insights into reproductive isolation within the pelagic copepod Pleuromamma abdominalis with high genetic diversity using genome-wide SNP data2020

    • Author(s)
      Junya Hirai
    • Journal Title

      Marine Biology

      Volume: 167 Issue: 1 Pages: 1-1

    • DOI

      10.1007/s00227-019-3618-x

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Possible aplanochytrid (Labyrinthulea) prey detected using 18S metagenetic diet analysis in the key copepod species <i>Calanus sinicus</i> in the coastal waters of the subtropical western North Pacific2018

    • Author(s)
      Junya Hirai, Yoko Hamamoto, Daiske Honda, Kiyotaka Hidaka
    • Journal Title

      Plankton and Benthos Research

      Volume: 13 Issue: 2 Pages: 75-82

    • DOI

      10.3800/pbr.13.75

    • NAID

      130006744038

    • ISSN
      1880-8247, 1882-627X
    • Year and Date
      2018-05-30
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Insights into reproductive isolation within highly divergent copepod Pleuromamma abdominalis using genome-wide SNP data2020

    • Author(s)
      Junya Hirai
    • Organizer
      Ocean Science Meeting
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Reproductive isolation in oceanic copepods revealed by genome-wide SNP data2018

    • Author(s)
      Hirai J
    • Organizer
      PICES Annual Meeting
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Feeding habits and diet composition of the larval Pacific Bluefin tuna larvae in the two nursery grounds2018

    • Author(s)
      Kodama T, Tawa A, Ishihara T, Hirai J, Oshimo S
    • Organizer
      4th CLIOTOP Symposium
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] The importance of the north Satsunan area, southern Japan as the spawning and nursery ground for small pelagic fish2018

    • Author(s)
      Kume G, Kobari T, Ichinomiya M, Komorita T, Hirai J, Aita-Noguchi M, Hyodo F, Takeda T, Shigemura T, Kuroda H, Yoshinaga S, Nakaya K, Narumi A
    • Organizer
      PICES Annual Meeting
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] The assemblages and feeding habits of larval fish in the Kuroshio and the adjacent waters, southern Japan2018

    • Author(s)
      Yoshinaga S, Kume G, Yamanoue K, Tanonaka N, Kobari T, Hirai J, Aita-Noguchi M, Hyodo F
    • Organizer
      PICES Annual Meeting
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Trophic sources and linkages of mesozooplankton and fish larvae in the Kuroshio based on metabarcoding analysis2018

    • Author(s)
      Sato I, Kobari T, Yoshinaga S, Kume G, Hirai J
    • Organizer
      PICES Annual Meeting
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Feeding habits of larval fish in the mouth of Kagoshima Bay, southern Japan2018

    • Author(s)
      Kuroda H, Kume G, Kobari T, Takeda T, Ichinomiya M, Komorita T, Hirai J, Aita-Noguchi M, Hyodo F
    • Organizer
      PICES Annual Meeting
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Transpaficic distribution of micronektonic fish community in the subtropical open water2018

    • Author(s)
      Nagatomo Y, Takahashi K, Sado T, Miya M, Hashihama F, Horii S, Hirai J
    • Organizer
      PICES Annual Meeting
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 鹿児島湾湾口部における魚類仔魚の摂餌生態2018

    • Author(s)
      黒田大海・久米元・小針統・竹田力・一宮睦雄・小森田智大・平井惇也・相田真希・兵藤不二夫
    • Organizer
      日本海洋学会秋季大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 鹿児島県南部の黒潮周辺海域における魚類仔魚の群集構造と摂餌生態2018

    • Author(s)
      吉永尚平・久米元・山之上香織・田野中里佳・小針統・平井惇也・相田真希・兵藤不二夫
    • Organizer
      日本海洋学会秋季大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 小型浮魚類の産卵場及び成育場としての北部薩南海域の重要性について2018

    • Author(s)
      久米元・小針統・一宮睦雄・小森田智大・平井惇也・相田真希・兵藤不二夫・竹田力・重村太一・黒田大海・吉永尚平・中屋慧・鳴海敦
    • Organizer
      日本海洋学会秋季大会
    • Related Report
      2018 Research-status Report
  • [Presentation] メタ18S解析による黒潮周辺海域表層の尾虫類の食性分析2018

    • Author(s)
      日高清隆・平井惇也・鈴木光次
    • Organizer
      日本ベントス学会・日本プランクトン学会合同大会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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