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A New Approach to Optimize Feeding of Fish: Elucidation of Organism Defense Mechanism by Autophagy

Research Project

Project/Area Number 18K14520
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 40040:Aquatic life science-related
Research InstitutionKyushu University (2020)
Ehime University (2018-2019)

Principal Investigator

Mohapatra Sipra  九州大学, 農学研究院, 学術研究員 (80715441)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
KeywordsAutophagy / Disease / Liver / fish / hexokinase / Red Sea bream / Cell death / liver / disease / E. tarda / Irridovirus / mitophagy / Hexokinase / estrogen / Fish / estrogen receptor / autophagy / オートファジー
Outline of Final Research Achievements

Autophagy, cells own repair mechanism, promotes health and longevity in various animals. In this study, we aimed to understand the role of autophagy, especially the pro-autophagic hexokinase (HK) genes, in fish disease management. We identified four HKs (HKI, HKII, HKIII & HKIV), characterized their effects in in vitro and ex vivo liver culture, analyzed the promoter activity in vitro, conducted Edwardsiella tarda challenge experiment, and performed the Chromatin immunoprecipitation analysis. We found that, fish HKs have high sequence similarity with mammalian ones, prevalent in liver and other steroidogenic tissues and was affected by starvation and early stages of E. tarda infection. Interestingly, only HKII showed a sex-biased and estrogen-dependent activity via several anti (mTOR) and pro(AMPK and ULK)-autophagic genes. Further analysis confirmed that HKII-mediated mitophagy are also sex biased, confirming the fact that HKII has significance in gender dependent female autophagy.

Academic Significance and Societal Importance of the Research Achievements

本研究で明らかにしたヘキソキナーゼとその関連因子は、魚類の健康状態を調べるための優れた指標となることから、環境に配慮した養殖魚の疾病予防対策および養殖生産性の向上に貢献するとともに、消費者に対するより安心で安全な水産物の提供に繋がる。また、魚類において見出したオートファジー機構の性差は、人を含めた脊椎動物における疾患や病態の性による差異にかかわる分子・細胞機構の理解に寄与すると考えられる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (6 results)

All 2020 2019 2018

All Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (3 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results)

  • [Journal Article] Estrogen and estrogen receptors chauffeur the sex-biased autophagic action in liver2020

    • Author(s)
      Sipra Mohapatra, Tapas Chakraborty, Sonoko Shimizu, Kayoko Ohta, Yoshitaka Nagahama, Kohei Ohta
    • Journal Title

      Cell Death & Differentiation

      Volume: NA Issue: 11 Pages: 3117-3130

    • DOI

      10.1038/s41418-020-0567-3

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Estrogen receptor β2 oversees germ cell maintenance and gonadal sex differentiation in medaka, Oryzias latipes2019

    • Author(s)
      2.Tapas Chakraborty, Sipra Mohapatra, Lin yan Zhou, Takahiro Matsubara, Taisen Iguchi, Yoshitaka Nagahama
    • Journal Title

      Stem Cell Reports

      Volume: 13 Issue: 2 Pages: 419-433

    • DOI

      10.1016/j.stemcr.2019.07.013

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Molecular cloning, characterization and expression analysis of complement components in red sea bream (Pagrus major) after Edwardsiella tarda and red sea bream Iridovirus (RSIV) challenge2018

    • Author(s)
      Noman Reza Mohammad Ali、Mohapatra Sipra、Shimizu Sonoko、Kitamura Shin-Ichi、Harakawa Shogo、Kawakami Hidemasa、Nakayama Kei、Sawayama Eitaro、Matsubara Takahiro、Ohta Kohei、Chakraborty Tapas
    • Journal Title

      Fish & Shellfish Immunology

      Volume: 82 Pages: 286-295

    • DOI

      10.1016/j.fsi.2018.08.027

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Sex and sex steroids are eminent regulators of fish autophagy2019

    • Author(s)
      Sipra Mohapatra, Tapas Chakraborty, Takahiro Matsubara, Kohei Ohta
    • Organizer
      10th International Conference on Fisheries and Aquaculture, Toronto, Canada
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Comparative transcriptomics and Edwardsiellosis detection in fish: a study using medaka and Red Sea bream2019

    • Author(s)
      Tapas Chakraborty, Sipra Mohapatra, Sonoko Shimizu, Takahiro Matsubara, Kiyoshi Naruse, Kohei Ohta
    • Organizer
      10th International Conference on Fisheries and Aquaculture, Toronto, Canada
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Exploring the potentiality of autophagy in fish fertility.2018

    • Author(s)
      Tapas Chakraborty, Sipra Mohapatra, Dipak Pandey, Takahiro Matsubara, Kohei Ohta
    • Organizer
      11th International Symposium on Reproductive Physiology of Fish (ISRPF), Manaus, Brazil, 2018.
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2022-01-27  

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