| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
Autophagy, cells own repair mechanism, promotes health and longevity in various animals. In this study, we aimed to understand the role of autophagy, especially the pro-autophagic hexokinase (HK) genes, in fish disease management. We identified four HKs (HKI, HKII, HKIII & HKIV), characterized their effects in in vitro and ex vivo liver culture, analyzed the promoter activity in vitro, conducted Edwardsiella tarda challenge experiment, and performed the Chromatin immunoprecipitation analysis. We found that, fish HKs have high sequence similarity with mammalian ones, prevalent in liver and other steroidogenic tissues and was affected by starvation and early stages of E. tarda infection. Interestingly, only HKII showed a sex-biased and estrogen-dependent activity via several anti (mTOR) and pro(AMPK and ULK)-autophagic genes. Further analysis confirmed that HKII-mediated mitophagy are also sex biased, confirming the fact that HKII has significance in gender dependent female autophagy.
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