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Gonadal construction and GnRH action mechanism in squid sexual differentiation and maturation

Research Project

Project/Area Number 18K14521
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 40040:Aquatic life science-related
Research InstitutionNagasaki University

Principal Investigator

Murata Ryosuke  長崎大学, 海洋未来イノベーション機構, 助教 (40809159)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsアオリイカ / カミナリイカ / 卵巣 / 精巣 / 性分化 / 性成熟 / GnRH-like peptide / 性ステロイドホルモン / 産卵制御要因 / 温度 / 性決定 / 不妊化 / 性特異的遺伝子 / GnRH / 性ホルモン / イカ類
Outline of Final Research Achievements

Histological examination of the gonad sex differentiation process of Kisslip cuttlefish, Sepia lycidas, revealed that the gonad sex differentiation of this species begins before hatching. As a result of investigating the fluctuation of GnRH-like expression in the brain associated with sexual differentiation of the gonad by qPCR and immunohistochemistry, no difference between male and female was observed in the expression levels, and the involvement of GnRH-like in sexual differentiation was unknown. It was clarified that the embryogenesis of this species progresses faster at higher water temperatures in the water temperature zone above 18 ° C. As a result of investigating changes in GnRH-like expression in the brain associated with gonad development of Bigfin reef squid, Sepioteuthis lessoniana, by qPCR, no association with ovarian development was observed, but association with testis development was observed, suggesting the involvement of GnRH-like on their spermatogenesis.

Academic Significance and Societal Importance of the Research Achievements

本研究により、海洋環境変動によるイカ類の生殖機能への生理的な影響を評価する際の基盤となる知見を得ることができた。 また、本研究により得られたイカ類の生殖生理メカニズムに関する基礎知見は、イカ類の増養殖技術開発を行う際に必須となる。以上の点において本研究により得られた成果はイカ類資源の持続可能な利用と増殖への発展が期待される。 無脊椎動物に広く存在が知られているGnRH-likeは、その具体的な機能に関する知見が不足している。本研究成果によりイカ類に関してはGnRH-likeは性分化への関与は少なく、性成熟に関しては精子形成への関与が示されたことから頭足類の生殖生理学的側面の理解が深まった。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (9 results)

All 2021 2020 2019 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (3 results) (of which Int'l Joint Research: 1 results) Remarks (4 results)

  • [Journal Article] Expression profile of GnRH-like peptide during gonadal sex differentiation in the cephalopod kisslip cuttlefish, Sepia lycidas2021

    • Author(s)
      Murata Ryosuke、Mushirobira Yuji、Tanaka Yoshiaki、Soyano Kiyoshi
    • Journal Title

      General and Comparative Endocrinology

      Volume: 304 Pages: 113718-113718

    • DOI

      10.1016/j.ygcen.2021.113718

    • NAID

      120006956176

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Gonadal sex differentiation and development during early ontogenesis in the breeding kisslip cuttlefish (Sepia lycidas)2019

    • Author(s)
      Ryosuke Murata, Yuji Mushirobira, Takeshi Fujita, Kiyoshi Soyano
    • Journal Title

      Hellion

      Volume: 5 Issue: 6 Pages: e01948-e01948

    • DOI

      10.1016/j.heliyon.2019.e01948

    • NAID

      120006987681

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] カミナリイカの性成熟に伴う脳内 GnRH 発現解析2020

    • Author(s)
      村田良介 莚平裕次 征矢野清
    • Organizer
      令和2年度日本水産学会春季大会
    • Related Report
      2020 Research-status Report
  • [Presentation] カミナリイカの性分化に伴う脳内GnRHの発現解析2019

    • Author(s)
      村田良介・莚平裕次・征矢野清
    • Organizer
      令和元年度日本水産学会秋季大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Gonadal sex differentiation and the expression pattern of GnRH in the brain of kisslip cuttlefish, Sepia lycidas2019

    • Author(s)
      Ryosuke Murata, Yuji Mushirobira, and Kiyoshi Soyano
    • Organizer
      The 16th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Remarks] 環境生殖生理学研究室HP

    • URL

      https://sites.google.com/site/muratalabhome/home?authuser=0

    • Related Report
      2021 Annual Research Report
  • [Remarks] 研究室HP

    • URL

      https://sites.google.com/site/muratalabhome/

    • Related Report
      2020 Research-status Report
  • [Remarks] 環東シナ海環境資源研究センターHP

    • URL

      http://www-mri.fish.nagasaki-u.ac.jp/index.html

    • Related Report
      2019 Research-status Report 2018 Research-status Report
  • [Remarks] 環境生殖生理学研究室HP

    • URL

      https://sites.google.com/site/muratalabhome/

    • Related Report
      2019 Research-status Report 2018 Research-status Report

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Published: 2018-04-23   Modified: 2023-01-30  

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