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Investigation of assembly mechanism of placenta by retroelement

Research Project

Project/Area Number 18K14566
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 42010:Animal production science-related
Research InstitutionTokyo University of Pharmacy and Life Science (2019)
The University of Tokyo (2018)

Principal Investigator

Kusama Kazuya  東京薬科大学, 薬学部, 助教 (30579149)

Project Period (FY) 2018-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords胎盤 / トロホブラスト / 内在性レトロウイルス因子 / 細胞融合 / 着床 / エピジェネティクス / ウシ / 内在性レトロウイルス 因子 / 内在性レトロウイルス
Outline of Final Research Achievements

Placental formation requires trophoblast cell fusion in many mammalian species. In the bovine species, endogenous retroviruses (ERVs) proteins are expressed in fused trophoblast cells. ERVs are thought to induce cell-cell fusion in the bovine species, but molecular mechanisms by which ERVs are transcribed have not been well characterized. To investigate the mechanism by which ERVs were expressed in fused trophoblast cells, we performed global RNA sequencing analyses with bovine trophoblast cells, identifying important genes related by placentation. Of these, several genes regulated the expression of ERVs. These indicate that identifying factors could regulate trophoblast fusion essential for bovine placentation.

Academic Significance and Societal Importance of the Research Achievements

本邦において様々な生産技術の開発や研究が行われているが、ウシの妊娠率は年々低下の一途をたどっており、その改善には至っていない。この原因の一つとして胎盤の形成不全が考えられる。本研究は、この胎盤の形成メカニズムを、内在性レトロウイルス因子(ERVs)に着目し明らかにしようと試みた。その結果、ERVsと胎盤形成を調節する因子を同定した。これは、胎盤形成不全による早期胚死滅の原因解明及び、その抑止につながり、学術領域だけでなく、畜産現場にも有益な効果を与えることが出来る。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (5 results)

All 2020 2019 2018

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (3 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] IFNT-independent effects of intrauterine extracellular vesicles (EVs) in cattle2020

    • Author(s)
      Nakamura Keigo、Kusama Kazuya、Ideta Atsushi、Imakawa Kazuhiko、Hori Masatoshi
    • Journal Title

      Reproduction

      Volume: 159 Issue: 5 Pages: 503-511

    • DOI

      10.1530/rep-19-0314

    • NAID

      130007925663

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of miR-98 in intrauterine extracellular vesicles on maternal immune regulation during the peri-implantation period in cattle2019

    • Author(s)
      Nakamura Keigo、Kusama Kazuya、Ideta Atsushi、Kimura Koji、Hori Masatoshi、Imakawa Kazuhiko
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 20330-20330

    • DOI

      10.1038/s41598-019-56879-w

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] ウシ胎盤細胞融合における内在性レトロウイルス遺伝子の発現調節機構の解明2019

    • Author(s)
      草間 和哉,田村 和広 ,堀 正敏 ,今川 和彦
    • Organizer
      第112回 日本繁殖生物学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 子宮内エクソソーム miR-98 による胚接着時の母体免疫系の抑制2019

    • Author(s)
      中村 圭吾,草間 和哉,出田 篤司,今川 和彦,堀 正敏
    • Organizer
      第112回 日本繁殖生物学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Bovine endogenous retroviral genes necessary for the trophoblast fusion are up-regulated by PPARG and cAMP after conceptus implantation2018

    • Author(s)
      Kazuya Kusama, Rulan Bai, Masako Suzuki, John Greally, Kazuhiko Imakawa
    • Organizer
      Society for the study of Reproduction (SSR) 51st Annual Meeting
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2021-02-19  

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