| Project/Area Number |
18K14568
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 42010:Animal production science-related
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| Research Institution | Kobe University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ニワトリ / インスリン / IGF-1 / 骨格筋 / Akｔ / S6 |
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| Outline of Final Research Achievements |
To clarify effects of skeletal muscle-derived IGF-1 on muscle differentiation and regulation of protein metabolism, I examined the expression of muscle differentiation-related genes and phosphorylation rates of Akt and Erk in chicken embryonic myoblasts and myotubes treated wiht IGF-1 siRNA. The treatment of IGF-1 siRNA significantly decreased the mRNA levels in the myoblasts and the phosphorylation rates in the myotubes. These results suggest that skeletal muscle-derived IGF-1 plays an important role in regulating the expression of muslce differentiation-related genes and protein metabolism in the skeletal muscle.
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| Academic Significance and Societal Importance of the Research Achievements |
骨格筋IGF-1は骨格筋の成長（筋形成と筋肥大）に重要であると考えられているが、それは骨格筋にIGF-1を過剰発現させた実験動物、及び筋細胞の培養液中にIGF-1を添加したin vitro実験等によって示唆されていることであり、骨格筋で本来作られるだけのIGF-1が筋分化や骨格筋のタンパク質代謝調節に影響を及ぼし得るのか不明であった。本研究により、骨格筋が作るIGF-1は、筋分化や骨格筋のタンパク質代謝調節に関与していることが示された。
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