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Unveiling the epigenetic mechanism of cellular differentiation in undifferentiated canine hemangiosarcoma cells

Research Project

Project/Area Number 18K14575
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 42020:Veterinary medical science-related
Research InstitutionHokkaido University

Principal Investigator

Aoshima Keisuke  北海道大学, 獣医学研究院, 助教 (90745069)

Project Period (FY) 2018-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords血管肉腫 / エピジェネティクス / ヒストン脱メチル化 / 腫瘍 / イヌ / がん幹細胞
Outline of Final Research Achievements

We found that histone demethylase KDM2B highly expressed in canine hemangiosarcoma cell lines and clinical samples. KDM2B knockdown halted cell growth and induced apoptosis in vitro. Furthermore, KDM2B knockdown in the tumors inoculated in immunodeficient mice let the tumors started shrinking. Based on these results, we confirmed that KDM2B is responsible for tumor cell growth and survival.

Academic Significance and Societal Importance of the Research Achievements

本研究によりKDM2Bがイヌ血管肉腫の増殖・生存に必須であることが明らかとなった。イヌの血管肉腫は高頻度に発生する悪性腫瘍であるにも関わらず,現段階で有効な治療法がない。今回,免疫不全マウスを用いた実験で,KDM2Bノックダウンは血管肉腫を退縮させることが明らかとなった。このことは,KDM2Bが血管肉腫に対する有効な治療標的であることを示唆するものであり,血管肉腫の新たな治療法を開発する上での基礎となる研究成果である。今後はKDM2Bの役割を更に詳細に研究し,治療法開発に向けた知見を集める予定である。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (4 results)

All 2019 2018

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (3 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] High drug efflux pump capacity and low DNA damage response induce doxorubicin resistance in canine hemangiosarcoma cell lines2019

    • Author(s)
      Morita Atsuya、Aoshima Keisuke、Gulay Kevin Christian Montecillo、Onishi Shinichi、Shibata Yuki、Yasui Hironobu、Kobayashi Atsushi、Kimura Takashi
    • Journal Title

      Research in Veterinary Science

      Volume: 127 Pages: 1-10

    • DOI

      10.1016/j.rvsc.2019.09.011

    • NAID

      120006937029

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] The Role of Histone Lysine Demethylase 2B (KDM2B) in Endothelial Cell Carcinogenesis2019

    • Author(s)
      K.C.M. Gulay, K. Ashima, A. Kobayashi, and T. Kimura
    • Organizer
      The 7th Sapporo Summer Symposium for One Health
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis of the KDM2B-mediated Regulation of the Growth, Development, and Maintenance of Canine Hemangiosarcoma.2018

    • Author(s)
      Gulay, K.C.M., Aoshima, K., Kobayashi, A., and Kimura, T.
    • Organizer
      The 6th SaSSOH seminar for One Health. Sapporo, Japan, 2018.
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Analysis of the KDM2B-mediated Regulation of the Growth, Development, and Maintenance of Canine Hemangiosarcoma2018

    • Author(s)
      Gulay, K.C.M., Aoshima, K., Kobayashi, A., and Kimura, T.
    • Organizer
      第41回 日本分子生物学会,横浜
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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