Development of bladder and ureteral substitution using collagenous tissue membrane (biosheet) in dogs

• Project/Area Number: 18K14592
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 42020:Veterinary medical science-related
• Research Institution: Osaka Prefecture University
• Principal Investigator: Funayama Marina 大阪府立大学, 生命環境科学研究科, 客員研究員 (30713599)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 泌尿器疾患 / 犬

Outline of Final Research Achievements

In-body tissue architecture technology was a novel and practical technique for regenerative medicine based on the tissue encapsulation phenomenon of foreign materials in patients. In this study, we prepared sheet-like collagenous tissues called "Biosheets" and tube-like collagenous tissues called "Biotubes" as reconstruction materials for urinary bladder and ureter. Each of Biosheets and Biotubes were implanted in the urinary bladder or ureter without damage at macroscopic levels to the tissues. Abdominal ultrasonography showed the occlusion of Biotubes until 3 weeks after implantation. Meanwhile, no disruption of Biosheets was observed until 12 weeks after implantation. Histological analyses revealed the luminal surface of Biosheets were covered with a multicellular layer of urothelium cells and αSMA positive muscle cells were observed at the margin of Biosheets at 12 weeks after implantation.

Academic Significance and Societal Importance of the Research Achievements

生体内組織形成術にて作製した移植用組織体は、サイズや形状の自由度が高く、比較的安価に提供できることから、伴侶動物の泌尿器治療に応用できる可能性がある。本研究では、自己の細胞やマトリックスから構成されたバイオシートおよびバイオチューブが、膀胱および尿管において良好な移植操作性を有することを示した。またバイオシートは伴侶動物の膀胱再建において、経済性に優れた移植用組織体となり得る可能性を示した。

Research Products

• [Journal Article] Urinary bladder reconstruction using autologous collagenous connective tissue membrane “Biosheet” induced by in-body tissue architecture: A pilot study (2020)
  • Author(s): Iimori Yasumasa、Iwai Ryosuke、Nagatani Kengo、Inoue Yuka、Funayama-Iwai Marina、Okamoto Mari、Nakata Mio、Mie Keiichiro、Nishida Hidetaka、Nakayama Yasuhide、Akiyoshi Hideo
  • Journal Title: Regenerative Therapy Volume: 15 Pages: 274-280
  • DOI: 10.1016/j.reth.2020.10.006
  • Peer Reviewed / Open Access
• [Presentation] Long term outcomes of iBTA-induced allgenic Biosheets as a diaphragmatic hernia repair material in a beagle model. (2019)
  • Author(s): Iimori Y, Nagatani K, Terazawa T, Inoue Y, Nakata M, Okamoto M, Funayama M, Mie K, Nishida H, Nakayama Y, Akiyoshi H
  • Organizer: The 6th Asian Meeting of Animal Medicine Specialties
  • Int'l Joint Research
• [Presentation] 犬における生体内組織形成術を用いた新規泌尿器修復材の検討 (2018)
  • Author(s): 飯盛安真、内藤一真、長谷健吾、船山麻理菜、岡本茉里、三重慧一郎、中山泰秀、秋吉秀保
  • Organizer: 第96回日本獣医麻酔外科学会
• [Presentation] Efficacy and safety of iBTA-induced autologous “Biosheets” as a urological repair material in a dog model. (2018)
  • Author(s): Iimori Y, Nagatani K, Inoue Y, Iwai R, Okamoto M, Funayama M, Mie K, Nishida H, Nakayama Y, Akiyoshi H.
  • Organizer: 8th Annual AiSVS Congress
  • Int'l Joint Research