| Project/Area Number |
18K14656
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 43030:Functional biochemistry-related
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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Principal Investigator |
Laurino Paola 沖縄科学技術大学院大学, タンパク質工学・進化ユニット, 准教授 (90812256)
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| Project Period (FY) |
2018-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | S-adenosyl methionine / directed evolution / Methionine / adenosyltransferase / RNA methyltransferase / In-vitro / compartimentalisation / Coenzyme engineering / SAM analogues / methionine / Methyltransferases / kinetic assays / coenzyme engineering / Protein Engineering / Directed evolution / Cofactors |
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| Outline of Final Research Achievements |
In this project we were aiming to replace the coenzyme S-Adenosyl Methionine (SAM) with a synthetic coenzyme carrying a different transferable group and nucleoside ; to develop a selection method for the RNA methyltransferase and finally to engineer a RNA methyltransferase to utilize the synthetic cofactor in a orthogonal manner.
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| Academic Significance and Societal Importance of the Research Achievements |
Herein we aimed to 1. generate an unique synthetic biology tool that sheds light on the role of mRNA in vivo 2. overcome the challenge of engineering a coenzyme binding pocket without disturb the substrate binding pocket 3. shade light on the coenzyme evolution.
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