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A method for identifying the mitoribosome interactome

Research Project

Project/Area Number 18K14674
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 43050:Genome biology-related
Research InstitutionKyoto University

Principal Investigator

Imami Koshi  京都大学, 薬学研究科, 特定研究員(特任講師) (30528344)

Project Period (FY) 2018-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2019: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywordsプロテオミクス / ミトコンドリア / リボソーム / 翻訳 / 質量分析
Outline of Final Research Achievements

The mitoribosome is specialized for producing proteins composed of the OXIPHOS complex in mitochondria. Protein production by mitoribosomes is an essential process in all living organisms and involves the mitochondria disease. However, our understanding about mitoribosomes is still limited. In this study, we devised a method to systematically capture the mitoribosome interactome, and reveal an overview of proteins involved in the translation and biogenesis for mitoribosoems.

Academic Significance and Societal Importance of the Research Achievements

ミトコンドリアは細胞内のエネルギー生産に必須のオルガネラであり、その機能障害はパーキンソン病などの疾病の原因となる。さらに、ミトコンドリア病に関連する遺伝子変異はミトコンドリア内の翻訳装置に異常をもたらすことが知られている。本研究で明らかにしたミトコンドリア翻訳系に関わるタンパク質の系統的な同定は、ミトコンドリア病や基本的なミトコンドリア機能の理解に貢献すると考えられる。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (9 results)

All 2019 2018

All Journal Article (3 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (6 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results)

  • [Journal Article] Measuring protein synthesis during cell cycle by Azidohomoalanine (AHA) labeling and flow cytometric analysis.2019

    • Author(s)
      Imami K, Yasuda T.
    • Journal Title

      Bio-Protocol

      Volume: 1 Issue: 8 Pages: 1-7

    • DOI

      10.21769/bioprotoc.3215

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of proteins and miRNAs that specifically bind an mRNA in vivo2019

    • Author(s)
      Theil Kathrin、Imami Koshi、Rajewsky Nikolaus
    • Journal Title

      Nature Communications

      Volume: 10 Issue: 1 Pages: 1-13

    • DOI

      10.1038/s41467-019-12050-7

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Phosphorylation of the Ribosomal Protein RPL12/uL11 Affects Translation during Mitosis2018

    • Author(s)
      Imami Koshi、Milek Miha、Bogdanow Boris、Yasuda Tomoharu、Kastelic Nicolai、Zauber Henrik、Ishihama Yasushi、Landthaler Markus、Selbach Matthias
    • Journal Title

      Molecular Cell

      Volume: 72 Issue: 1 Pages: 84-98

    • DOI

      10.1016/j.molcel.2018.08.019

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] ”Functionality”を有するリン酸化部位を相関解析から同定する2019

    • Author(s)
      今見考志, 石濱泰
    • Organizer
      第92回日本生化学会大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 転写後・翻訳制御機構の解明に向けたプロテオミクス基盤技術の開発2019

    • Author(s)
      今見考志
    • Organizer
      日本プロテオーム学会2019年大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 「翻訳」からみる遺伝子発現制御の理解: シグナル伝達制御とのクロストーク2019

    • Author(s)
      今見考志
    • Organizer
      第16回 北里疾患プロテオーム研究会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 転写後・翻訳制御のプロテオミクス2019

    • Author(s)
      今見考志
    • Organizer
      RNAフロンティアミーティング2019
    • Related Report
      2019 Annual Research Report
  • [Presentation] “Ribosomics” reveals a novel mechanism for translational regulation2019

    • Author(s)
      今見考志・Miha Milek・Boris Bogdanow・保田朋波流・石濱泰・Markus Landthaler・Matthias Selbach
    • Organizer
      国際シンポジウム Proteins: From the Cradle to the Grave
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Ribosomics reveals a novel mechanism for translational regulation2019

    • Author(s)
      Imami K, Mielk M, Yasuda T, Bogdnow B, Yasuda T, Kastelic N, Zauber N, Ishihama Y, Landthaler M, Selbach M
    • Organizer
      第20回武田科学振興財団生命科学シンポジウム RNAネオバイオロジー
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2021-02-19  

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