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Approach to the problem of phosphorylation routing with novel ATP analogs

Research Project

Project/Area Number 18K14683
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 43060:System genome science-related
Research InstitutionThe University of Tokyo

Principal Investigator

Ode Koji  東京大学, 大学院医学系研究科(医学部), 講師 (40612122)

Project Period (FY) 2018-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords質量分析 / リン酸化 / 天然変性領域 / ASキナーゼ
Outline of Final Research Achievements

The aim of this study is to establish an experimental system to systematically analyze the interdependence of multiple phosphorylation sites that proceed in a kinase-specific manner. Technically, we were unable to evaluate the function of the novel ATP analogues using AS kinase, which remains a challenge. On the other hand, for CaMKII, we evaluated the new multiple phosphorylation sites and their functions, presenting the possibility that the kinase is regulated by both activity promotion and repression at longer time scales than previously well understood.

Academic Significance and Societal Importance of the Research Achievements

質量分析を始めとした網羅的手法によって、特定の基質にリン酸化サイトが多数見出されている現状を鑑み、本研究では細胞内で特定の基質の多重リン酸化の相互依存性すなわち「経路」を明らかにする試みを通して、タンパク質内の複数のリン酸化サイトが織りなす経路選択問題(routing)を扱う視点を提示した。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (9 results)

All 2020 2019 2018

All Journal Article (2 results) Presentation (7 results) (of which Invited: 7 results)

  • [Journal Article] 概日時計と睡眠2019

    • Author(s)
      大出 晃士、上田 泰己
    • Journal Title

      臨床神経科学

      Volume: 37 Pages: 770-773

    • Related Report
      2019 Annual Research Report
  • [Journal Article] 哺乳類概日時計の周期長決定要因を探る2019

    • Author(s)
      大出 晃士, 上田 泰己
    • Journal Title

      実験医学

      Volume: 37 Pages: 379-385

    • Related Report
      2018 Research-status Report
  • [Presentation] CaMKIIによる睡眠時間長制御2020

    • Author(s)
      大出晃士
    • Organizer
      第93回日本薬理学会年会(誌上開催)
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 哺乳類CaMKIIの酵素活性依存的な睡眠時間長制御2019

    • Author(s)
      大出晃士
    • Organizer
      第26回日本時間生物学会学術大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Residue-level characterization of mCRY1-dependent circadian control2019

    • Author(s)
      大出晃士
    • Organizer
      第92回日本生化学会大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 睡眠時間長制御因子としての哺乳類CaMKII活性2019

    • Author(s)
      大出晃士
    • Organizer
      日本睡眠学会第44回定期学術集会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 概日リズムを駆動する時計タンパク質の量的変動と質的変動2018

    • Author(s)
      大出晃士
    • Organizer
      The 45th Biological Mass Spectrometry Conference
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Structure-guided engineering of mCRY1 to elucidate the protein quality control for the determination of circadian clock speed2018

    • Author(s)
      大出晃士
    • Organizer
      第91回日本生化学会大会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Structure-guided engineering of mammalian Cryptochrome to elucidate the origin of 24-hour period2018

    • Author(s)
      大出晃士
    • Organizer
      第25回日本時間生物学会学術大会
    • Related Report
      2018 Research-status Report
    • Invited

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Published: 2018-04-23   Modified: 2021-02-19  

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