| Project/Area Number |
18K14701
|
|---|
| Research Category |
Grant-in-Aid for Early-Career Scientists
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
Basic Section 44010:Cell biology-related
|
|---|
| Research Institution | Kyushu University |
|---|
Principal Investigator |
FUKUDA MASAKO 九州大学, 農学研究院, 特任助教 (30750385)
|
|---|
| Project Period (FY) |
2018-04-01 – 2022-03-31
|
| Project Status |
Completed (Fiscal Year 2021)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | イネ種子 / 貯蔵タンパク質 / グルテリン / プロラミン / RNA結合タンパク質 / 小胞輸送 / 細胞内輸送 / イネ種子貯蔵タンパク質 / RNA binding protein / 共焦点レーザー顕微鏡 / RNA-IP / UVクロスリンキング / イネ / 変異体 / PB-ER / Cis-ER / RNA局在 / Tilling法 |
|---|
| Outline of Final Research Achievements |
1）A total of 14 allelic mutants of RNA binding protein (RBP)-A, I, J, K, and Q involved in the transport of prolamin mRNA from the nucleus to the endoplasmic reticulum were selected by the Tilling method. 2）Accumulation of prolamin was reduced in all rbp mutants. 3）RBP-A, RBP-J and RBP-Q were localized in the nucleus and prolamin granules（PB-ER）. 4）In the rbp-a, rbp-i, and rbp-q mutant seeds, the PB-ER were downsized. These results suggest that RBP-A, RBP-I and RBP-Q are involved in the accumulation of prolamin in PB-ER.
|
| Academic Significance and Societal Importance of the Research Achievements |
本研究より、各RNA結合タンパク質（RBP）変異体種子において、プロラミン蓄積の減少、及びプロラミン顆粒の小型化が認められたことから、プロラミンの正常な蓄積には、小胞体からの輸送蓄積系(翻訳後制御)だけでなく、各RBPを介したプロラミンmRNAの核から小胞体（ER-PB）への輸送（転写後制御）が必要であることが示唆された。RBPが貯蔵タンパク質の輸送・蓄積に関与するという知見は得られていたが、更に確かなものとなった。
|
