| Project/Area Number |
18K14706
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 44010:Cell biology-related
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| Research Institution | National Institute of Health Sciences (2019)
The University of Tokyo (2018) |
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Principal Investigator |
Yoshiba Satoko 国立医薬品食品衛生研究所, 生化学部, 主任研究官 (70642213)
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| Project Period (FY) |
2018-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 中心小体複製 / PLK4 / STIL / SAS-6 / γTuRC / degron / 天然変性領域 / 中心体 / カートホイール構造 |
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| Outline of Final Research Achievements |
In the cell cycle, a single daughter centriole is formed next to the pre-existing centriole. The key questions that I addressed in this study are how PLK4, STIL and SAS-6 sequentially localize to the mother centriole and form a complex to initiate the formation of a new centriole. In this study I tried to understand the molecular mechanism how STIL and SAS-6 load to the PLK4 condensate, which self-assembles under the control of its auto-phosphorylation. By analyzing the interactions and molecular properties of these proteins, I obtained several new findings, which would provide a basis for the future research in this field.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、動物細胞において進化的に保存された細胞小器官である、中心小体の複製開始のしくみを分子的に明らかにしようとした。細胞内において、無秩序な分子の集合が、どのようにして秩序を持つ構造体の形成につながるのかは、細胞生物学における重要な問題の一つであるが、本研究において中心小体をモデルとして、問題提起および今後に繋がる研究ができたことは、広く基礎生物学研究において学術的意義があったと考える。
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