| Project/Area Number |
18K14715
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 44020:Developmental biology-related
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| Research Institution | Osaka University |
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Principal Investigator |
Castaneda Julio 大阪大学, 微生物病研究所, 特任助教(常勤) (00791659)
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| Project Period (FY) |
2018-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | spermatogenesis / sperm / N-DRC / sperm motility / male fertility / hydrocephalus / flagellum / cilia / fertility / sperm function / mice / CRISPR/Cas9 / spermatozoa |
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| Outline of Final Research Achievements |
My proposal set out the examine cilia and flagella formation by studying 3 components of the Nexin-Dynein Regulatory Complex (N-DRC). The N-DRC is a macromolecular protein complex that helps regulate the beating motion of flagella and cilia as well as their formation. Flagella and cilia are important for cell and organ function. The N-DRC is composed of 11 subunits (DRC1-11). Eight N-DRC components have been studied in the mouse that lead to various phenotypes such as abnormal organ development (DRC1), short sperm tails (Drc7), immotile sperm (Drc5/Tcte1), or no phenotype at all (Drc6/Fbxl13). The remaining genes yet to be studied include DRC2, 10 (Iqcd), and 11 (Iqca). Using CRISPR/Cas9, I managed to knockout all 3 genes. I found that Drc2 knockout mice are perinatal lethal and exhibit hydrocephalus. Drc11/Iqca mice are fertile with no apparent abnormalities, while Drc10/Iqcd analysis is still ongoing.
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| Academic Significance and Societal Importance of the Research Achievements |
The N-DRC is an important macromolecular complex that help regulate flagella and cilia formation and function. My research examined the last remaining components of the N-DRC that have yet to be studied. This research completes the analysis of all components of the N-DRC.
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