Understanding flagellum formation in mouse spermatozoa
Project/Area Number |
18K14715
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 44020:Developmental biology-related
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Research Institution | Osaka University |
Principal Investigator |
Castaneda Julio 大阪大学, 微生物病研究所, 特任助教(常勤) (00791659)
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Project Period (FY) |
2018-04-01 – 2020-03-31
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Project Status |
Completed (Fiscal Year 2019)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | spermatogenesis / sperm / N-DRC / sperm motility / male fertility / hydrocephalus / flagellum / cilia / fertility / sperm function / mice / CRISPR/Cas9 / spermatozoa |
Outline of Final Research Achievements |
My proposal set out the examine cilia and flagella formation by studying 3 components of the Nexin-Dynein Regulatory Complex (N-DRC). The N-DRC is a macromolecular protein complex that helps regulate the beating motion of flagella and cilia as well as their formation. Flagella and cilia are important for cell and organ function. The N-DRC is composed of 11 subunits (DRC1-11). Eight N-DRC components have been studied in the mouse that lead to various phenotypes such as abnormal organ development (DRC1), short sperm tails (Drc7), immotile sperm (Drc5/Tcte1), or no phenotype at all (Drc6/Fbxl13). The remaining genes yet to be studied include DRC2, 10 (Iqcd), and 11 (Iqca). Using CRISPR/Cas9, I managed to knockout all 3 genes. I found that Drc2 knockout mice are perinatal lethal and exhibit hydrocephalus. Drc11/Iqca mice are fertile with no apparent abnormalities, while Drc10/Iqcd analysis is still ongoing.
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Academic Significance and Societal Importance of the Research Achievements |
The N-DRC is an important macromolecular complex that help regulate flagella and cilia formation and function. My research examined the last remaining components of the N-DRC that have yet to be studied. This research completes the analysis of all components of the N-DRC.
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Report
(3 results)
Research Products
(8 results)
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[Journal Article] CRISPR/Cas9-based gene knockout in mice uncovers 13 testis- or epididymis-enriched genes individually dispensable for male reproduction2020
Author(s)
Jiang Sun,Yonggang Lu,Kaori Nozawa,Zoulan Xu, Akane Morohoshi,Julio M. Castaneda,Taichi Noda,Haruhiko Miyata, Ferheen Abbasi, Hossam H. Shawki ,Satoru Takahashi,Darius J. Devlin, Zhifeng Yu, Ryan M. Matzuk, Thomas X. Garcia, Martin M. Matzuk, Masahito Ikawa
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Journal Title
Biology of Reproduction
Volume: 102
Pages: 1-9
Related Report
Peer Reviewed / Open Access
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