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Single-cell Timelapse measurement of metabolic change during stem cell differentiation

Research Project

Project/Area Number 18K14721
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 44020:Developmental biology-related
Research InstitutionThe University of Tokyo (2019-2020)
Institute of Physical and Chemical Research (2018)

Principal Investigator

Yaginuma Hideyuki  東京大学, 大学院工学系研究科(工学部), 特任研究員 (10733222)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
KeywordsATP / 細胞イメージング / 代謝計測 / 蛍光プローブ / 蛍光イメージング / 代謝 / 幹細胞
Outline of Final Research Achievements

Intracellular energy production pathways, such as oxidative phosphorylation and glycolysis, are closely related to intracellular metabolic conditions. With conventional ATP measurement methods, it has been difficult to accurately quantify intracellular ATP concentration at the single cell level. In this study, we examined how the metabolic state changes depending on the culture and differentiation status of cells using a ratiometric fluorescent ATP indicator protein "QUEEN" that quantitatively measures the intracellular ATP concentration. In this study, we examined how the metabolic state of undifferentiated cells changes depending on their culture and differentiation status. We succeeded in finding that the metabolic state changes significantly depending on the culture state of the cells, and also elucidated the possibility of interaction of the metabolic state between embryonic stem cells.

Academic Significance and Societal Importance of the Research Achievements

ATPは、さまざまな細胞内の反応の進行に必要であり、細胞の代謝状態のパラメーターとして重要である。ATPのような代謝物質の濃度は細胞の運命決定に大きく関わっていると考えられる。本研究により、細胞が性質をかえていくに従って細胞内の代謝状態に多様な状態が現れることを単一細胞計測により明らかにした。また、細胞同士が作用しあうことによって代謝状態が変化する可能性も示された。これらの結果から、生物の細胞集団がどのように多様な細胞種が生み出されるか、またそれらが協調して個体を作り上げていくか、というメカニズムの解明に寄与したと考えられる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (8 results)

All 2019 2018 Other

All Int'l Joint Research (1 results) Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results)

  • [Int'l Joint Research] Lomonosov Moscow State University(ロシア連邦)

    • Related Report
      2018 Research-status Report
  • [Journal Article] A photostable fluorescent marker for the superresolution live imaging of the dynamic structure of the mitochondrial cristae.2019

    • Author(s)
      Wang C, Taki M, Sato Y, Tamura Y, Yaginuma H, Okada Y, Yamaguchi S.
    • Journal Title

      Proc Natl Acad Sci U S A.

      Volume: 116 Issue: 32 Pages: 15817-15822

    • DOI

      10.1073/pnas.1905924116

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A Method for Automatic Tracking of Cell Nuclei in 2D Epifluorescence Microscopy Image Sequences2018

    • Author(s)
      Kondratiev Alexandr Yu.、Yaginuma Hideyuki、Okada Yasushi、Sorokin Dmitry V.
    • Journal Title

      2018 Eighth International Conference on Image Processing Theory, Tools and Applications (IPTA)

      Volume: 1 Pages: 1-6

    • DOI

      10.1109/ipta.2018.8608156

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 細胞のエネルギー状態の定量的単一細胞解析を可能にするATPセンサー蛍光タンパク質“QUEEN”2019

    • Author(s)
      Hideyuki Yaginuma
    • Organizer
      第71回日本細胞生物学会大会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 定量的ATPイメージングを用いた細胞の代謝状態の空間的相関の解析2019

    • Author(s)
      Hideyuki Yaginuma
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 単一細胞ATP イメージングにより明らかになった不均一な代謝状況下での頑健なエネルギー量調節2018

    • Author(s)
      柳沼秀幸、岡田康志
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Quantitative ATP imaging as a tool for investigating distribution, cell cell correlation and rapid dynamics of energy level at single cell resolution.2018

    • Author(s)
      Hideyuki Yaginuma, Yasushi Okada
    • Organizer
      ASCB|EMBO 2018 meeting
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] A Method for Automatic Tracking of Cell Nuclei in 2D Epifluorescence Microscopy Image Sequences.2018

    • Author(s)
      Alexandr Yu. Kondratiev, Hideyuki Yaginuma, Yasushi Okada, Dmitry V. Sorokin
    • Organizer
      Eighth International Conference on Image Processing Theory, Tools and Applications (IPTA). 2018 Eighth International Conference on Image Processing Theory, Tools and Applications (IPTA). 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2022-01-27  

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