Functional analysis of an RNA methyltransferase in regulation of neural stem cell fate
| Project/Area Number |
18K14722
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 44020:Developmental biology-related
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Wu Quan 国立研究開発法人理化学研究所, 生命機能科学研究センター, 基礎科学特別研究員 (60812766)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | neural stem cell / rRNA methylation / epigenetics / translational regulation / RNA modificaiton / Brain development / Temporal patterning / RNA methltransferase / Single cell analysis / Brain Development / RNA Methylation / Neural Stem Cell |
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| Outline of Final Research Achievements |
Animal development is a highly coordinated temporal and spatial process. A clock mechanism has been often implicated in the temporal control of organ development. Accumulating evidence suggests the temporal state of tissues or tissue stem cells is mainly defined by the genome-wide epigenetic and transcriptional state as the clock, while little is known about what drives the postulated clock. In this work, we focused on temporal changes in embryonic neural stem cells (NSCs) identity during neocortical development (which sequentially generates different types of neurons and is considered a typical example of a developmental clock) and found that a methyltransferase of rRNA: Fibrillarin drives this clock through controlling translational efficiencies of epigenetic modifiers in the cerebral cortex primordium.
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| Academic Significance and Societal Importance of the Research Achievements |
In this study, we have identified the existence of a clock system in neural stem cells during brain development. By elucidating this mechanism, we can contribute to the understanding of the mechanisms of ageing in animals.
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Report
(4 results)
Research Products
(6 results)
