Molecular mechanisms of the redirection of the secretory pathway to form lineage-unique organelles

• Project/Area Number: 18K14738
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 44030:Plant molecular biology and physiology-related
• Research Institution: National Institute for Basic Biology
• Principal Investigator: Kanazawa Takehiko 基礎生物学研究所, 細胞動態研究部門, 助教 (60802783)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 油体 / 細胞板 / オルガネラ / 細胞骨格 / 膜交通 / 分泌経路 / ゼニゴケ / 転写因子

Outline of Final Research Achievements

The membrane traffic is the fundamental system to correctly localized the molecules via membrane-bounded intermediates. Although some lineages of eukaryotes are reported to have acquired lineage-specific organelles, the molecular mechanisms to pioneer the novel membrane trafficking pathways are unclear.
As a model of newly-acquired organelles, I analyzed the cell plates in dividing cells and the liverwort-specific oil body in Marchantia polymorpha. I identified that these organelles are formed by periodic redirection of the secretory pathway, which responsible for the transport to the extracellular space. The cytoskeleton is also re-organized in each process as well. By the mutant screening of the oil body biogenesis, MpERF13 was isolated as a master transcription factor to form oil bodies and to re-organize the cytoskeleton.

Academic Significance and Societal Importance of the Research Achievements

単膜系オルガネラの進化と多様化は、膜交通の鍵因子の遺伝子重複による数の増加とその後の機能分化が新しいオルガネラの獲得に繋がるというオルガネラパラロジー仮説が提唱されているが、この仮説を支持する実験的な証拠はほとんど示されていない。
全く機能の異なる細胞板と油体が、共通の膜交通経路の転用により形成されるという発見は、上述の仮説を強く支持しており、単膜系オルガネラの獲得原理の理解に向けた基礎生物学的な重要な知見が得られた。
また、苔類の油体は、生理活性の高い様々な化合物を蓄積しており、本研究で明らかになった油体形成の仕組みを活用することで、有用化合物の効率的な産生への貢献も期待される。

Research Products

• [Int'l Joint Research] Monash University(オーストラリア)
• [Int'l Joint Research] Max Planck Institute/Heidelberg University(ドイツ)
• [Int'l Joint Research] Universidad Nacional del Litoral(アルゼンチン)
• [Int'l Joint Research] Universidad Nacional del Litoral(アルゼンチン)
• [Int'l Joint Research] Monash University(オーストラリア)
• [Int'l Joint Research] Max Planck Institute(ドイツ)
• [Int'l Joint Research] Monash University(オーストラリア)
• [Int'l Joint Research] Gregor Mendel Institute(オーストリア)
• [Int'l Joint Research] Max Planck Institute(ドイツ)
• [Journal Article] The liverwort oil body is formed by redirection of the secretory pathway (2020)
  • Author(s): Kanazawa T., Morinaka H., Ebine K., Shimada T.L., Ishida S., Minamino N., Yamaguchi K., Shigenobu S., Kohchi T., Nakano A., Ueda T.
  • Journal Title: Nature Communications Volume: 11 Issue: 1 Pages: 6152-6152
  • DOI: 10.1038/s41467-020-19978-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The Arabidopsis V-ATPase is localized to the TGN/EE via a seed plant-specific motif (2020)
  • Author(s): Lupanga U., Rohrich R., Askani J., Hilmer S., Kiefer C., Krebs M., Kanazawa T., Ueda T., Schumacher K.
  • Journal Title: eLife Volume: 9
  • DOI: 10.7554/elife.60568
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Characterization of ancestral myosin XI from Marchantia polymorpha by heterologous expression in Arabidopsis thaliana (2020)
  • Author(s): Duan Zhongrui、Tanaka Misato、Kanazawa Takehiko、Haraguchi Takeshi、Takyu Akiko、Era Atsuko、Ueda Takashi、Ito Kohji、Tominaga Motoki
  • Journal Title: The Plant Journal Volume: 104 Issue: 2 Pages: 460-473
  • DOI: 10.1111/tpj.14937
  • Peer Reviewed
• [Journal Article] Oil Body Formation in Marchantia polymorpha Is Controlled by MpC1HDZ and Serves as a Defense against Arthropod Herbivores (2020)
  • Author(s): Romani F., Banic E., Florent S.N., Kanazawa T., Goodger J.Q.D., Mentink R.A., Dierschke T., Zachgo S., Ueda T., Bowman J.L., Tsiantis M., Moreno J.E.
  • Journal Title: Current Biology Volume: 30 Issue: 14 Pages: 2815-2828.e8
  • DOI: 10.1016/j.cub.2020.05.081
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Marchantia polymorpha, a New Model Plant for Autophagy Studies (2019)
  • Author(s): Norizuki T., Kanazawa T., Minamino N., Tsukaya H., Ueda T.
  • Journal Title: Frontiers in Plant Science Volume: 10 Pages: 935-935
  • DOI: 10.3389/fpls.2019.00935
  • Peer Reviewed / Open Access
• [Journal Article] The RopGEF KARAPPO Is Essential for the Initiation of Vegetative Reproduction in Marchantia polymorpha (2019)
  • Author(s): Hiwatashi T., Goh H., Yasui Y., Koh L.Q., Takami H., Kajikawa M., Kirita H., Kanazawa T., Minamino N., Togawa T., Sato M., Wakazaki M., Yamaguchi K., Shigenobu S., Fukaki H., Mimura T., Toyooka K., Sawa S., Yamato K.T.,Ueda T., Urano D., Kohchi T., Ishizaki K.
  • Journal Title: Current Biology Volume: 29 Issue: 20 Pages: 3525-3531
  • DOI: 10.1016/j.cub.2019.08.071
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] RAB GTPases in the Basal Land Plant Marchantia polymorpha (2018)
  • Author(s): Minamino Naoki、Kanazawa Takehiko、Era Atsuko、Ebine Kazuo、Nakano Akihiko、Ueda Takashi
  • Journal Title: Plant Cell Phys. Volume: in press Issue: 4 Pages: 850-861
  • DOI: 10.1093/pcp/pcy027
  • Peer Reviewed / Open Access
• [Presentation] 苔類ゼニゴケの油体発生に伴う分泌経路の方向転換制御機構 (2021)
  • Author(s): 金澤建彦, 上田貴志
  • Organizer: 第62回 日本植物生理学会年会
• [Presentation] 植物の新規オルガネラ獲得に伴う膜交通経路の制御機構 (2020)
  • Author(s): 金澤建彦, 上田貴志
  • Organizer: 日本植物学会第84回大会
• [Presentation] ゼニゴケの油体形成および輸送に関わる因子の探索 (2020)
  • Author(s): 金澤建彦, 上田貴志
  • Organizer: 第61回 日本植物生理学会年会
• [Presentation] ゼニゴケの油体形成および輸送に関わる因子の探索 (2020)
  • Author(s): 金澤建彦, 上田貴志
  • Organizer: 第61回日本植物生理学会年会
• [Presentation] 新規獲得オルガネラへの膜交通経路の開拓と制御機構 (2019)
  • Author(s): 金澤建彦, 上田貴志
  • Organizer: 日本植物学会第83回大会
• [Presentation] 光らない蛍光タンパク質 から学んだ教訓 (2019)
  • Author(s): 金澤建彦
  • Organizer: 日本植物学会第83回大会・関連集会
• [Presentation] Biogenesis and Morphogenesis of Oil Body (2019)
  • Author(s): Takehiko Kanazawa, Takashi Ueda
  • Organizer: Marchantia Workchop 2019
  • Int'l Joint Research
• [Presentation] 膜交通から探るゼニゴケ油体形成機構 (2019)
  • Author(s): 金澤建彦、上田貴志
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] Regulatory Mechanisms of Biogenesis of the Oil Body in Marchantia polymorpha (2019)
  • Author(s): Takehiko Kanazawa, Takashi Ueda
  • Organizer: Japan-Taiwan Plant Biology 2019
  • Int'l Joint Research
• [Presentation] A common strategy for organelle acquisition during plant evolution (2018)
  • Author(s): Takehiko Kanazawa, Takashi Ueda
  • Organizer: EMBO Workshop
  • Int'l Joint Research
• [Presentation] A shared framework of organelle acquisition during land plant evolution (2018)
  • Author(s): Takehiko Kanazawa, Takashi Ueda
  • Organizer: 21st ENPER meeting
  • Int'l Joint Research
• [Remarks] ゼニゴケの油体は分泌経路の方向転換によりつくられる
  • URL: https://www.nibb.ac.jp/pressroom/news/2020/12/01.html