| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Outline of Final Research Achievements |
Melanin-concentrating hormone (MCH) plays a role in various physiological functions such as feeding, emotion, and sleep via interactions with MCH receptor 1 (MCHR1), which is a membrane receptor. Recently, some GPCRs, including MCHR1, have been shown to localize to the membrane of primary cilia instead of the cell membrane. Previously, we have found that MCHR1-positive cilia are shortened by MCH stimulation in hRPE1 cells. In this study, we treated hPRE1 cells with inhibitors, siRNAs, and CRISPR-Cas9 and performed western blot and RNAseq analyses to determine signals and mediator molecules that are involved in ciliary shortening. We have also demonstrated that the MCH-MCHR1 system causes ciliary shortening in organotypic rat hippocampal slice cultures, rat primary hippocampal dissociated cultures, and hiPSC-derived cortical neurons via a similar mechanism. In conclusion, these results suggest that ciliary MCHR1 play a role in ciliary shortening.
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