Development of clearing method for plant tissues
| Project/Area Number |
18K14743
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 44040:Morphology and anatomical structure-related
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| Research Institution | Osaka University (2019)
Tokyo University of Science (2018) |
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Principal Investigator |
Sakamoto Yuki 大阪大学, 理学研究科, 助教 (00735483)
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| Project Period (FY) |
2018-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 透明化 / 顕微鏡 / 顕微鏡技術 / 植物 |
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| Outline of Final Research Achievements |
I developed a novel clearing method “iTOMEI” which rapidly and completely clears plant tissue maintaining fluorescence of fluorescent proteins. iTOMEI is composed of 4 steps : fixation, decolorization, reactivation of fluorescent protein, and unification of reflective index. This method can use for not only plant tissues such as Arabidopsis thaliana, Oryza sativa, and Marchantia polymorpha but also animal tissue.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究で開発された透明化技術iTOMEIは簡便かつ迅速に植物組織を透明化できることから、植物を用いた基礎研究のみならず応用的な研究にも広く用いられると考えられる。特に非破壊的に透明化が可能であることから、農作物における害虫や寄生虫、病原菌の発見や感染箇所の同定、作物の品種間の細胞形態、組織の内部構造の観察、比較をもとにより良い品種改良への足がかりにつながる。
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Report
(3 results)
Research Products
(30 results)
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[Journal Article] Intracellular localization of histone deacetylase HDA6 in plants.2019
Author(s)
Kurita, K., Sakamoto, Y., Naruse, S., Matsunaga, T. M., Arata, H., Higashiyama, T., Habu, Y., Utsumi, Y., Utsumi, C., Tanaka, M., Takahashi, S., Kim, J. M., Seki, M., Sakamoto, T., and Matsunaga, S.
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Journal Title
J PLANT RES
Volume: 132
Issue: 5
Pages: 629-640
DOI
NAID
Related Report
Peer Reviewed / Int'l Joint Research
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