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Cellular identity, systematics and biogeography of apicomplexan parasites Genotype-N and Gemmocystis infecting coral reefs

Research Project

Project/Area Number 18K14774
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 45030:Biodiversity and systematics-related
Research InstitutionHokkaido University

Principal Investigator

Wakeman Kevin  北海道大学, 高等教育推進機構, 助教 (70760221)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
KeywordsBiology / Coral reefs / Parasitology / parasites / soft coral / Okinawa / biodiversity / Phylogeny / Biodiversity / Marine Biology / Systematics / Biogeography / Apicomplexa / Parasites / Coral Reefs / Parasite / Molecular phylogeny / Histological analysis
Outline of Final Research Achievements

This work focused on sampling apicomplexans associated with coral reefs. Samples were collected from Okinawa and in locations around Southern Japan, including Ishigaki, Kagoshima and Hiroshima. Samples were processed for histology and PCR screening of apicomplexans using the 18S rDNA. Samples were also prepared for next generation sequencing, in order to gain a better understanding of the diversity of apicomplexans associated with these samples. In total over 80 species of coral were sampled and sent for sequencing. As a general result, we found that apicomplexans that associate with coral are broadly distributed, geographically and throughout coral reefs. To this end, coral in Japan have similar communities of apicomplexans to those found in other localities such as the Caribbean. While similar, these species are likely different, and appear to be unique to the waters around Japan.

Academic Significance and Societal Importance of the Research Achievements

社会的および科学的影響から、これらの結果はサンゴの生物学を理解するために重要であり、生物が寄生虫と持つ相互作用についての見通しを得るための最初のステップのいくつかです。言い換えれば、この作業はサンゴ礁の健康をよりよく管理するのに役立ちます。この研究からの重要な派生物の1つは、微生物叢と、これが特に気候変動時にサンゴの健康にどのように影響するかに関する研究です。健康なサンゴ微生物叢を構成するものを理解することは、サンゴの白化現象の予測因子となり、その影響を制限するのに役立つ可能性があります

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (10 results)

All 2019 2018 2017

All Journal Article (10 results) (of which Int'l Joint Research: 9 results,  Peer Reviewed: 9 results,  Open Access: 1 results)

  • [Journal Article] Morphological and molecular diversity of rissoellids (Mollusca, Gastropoda, Heterobranchia) from the Northwest Pacific island of Hokkaido, Japan.2019

    • Author(s)
      LEC Siaden, KC Wakeman, SC Webb, K Hasegawa, H Kajihara
    • Journal Title

      Zootaxa

      Volume: 4551 Issue: 4 Pages: 415-431

    • DOI

      10.11646/zootaxa.4551.4.2

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Dinoflagellate nucleus contains an extensive endomembrane network, the nuclear net2019

    • Author(s)
      GS Gavelis, M Herranz, KC Wakeman, C Ripken, S Mitarai, GH Gile, ...
    • Journal Title

      Scientific reports

      Volume: 9 Issue: 1 Pages: 839-839

    • DOI

      10.1038/s41598-018-37065-w

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] PCR and histology identify new bivalve hosts of Apicomplexan-X (APX), a common parasite of the New Zealand flat oyster Ostrea chilensis2019

    • Author(s)
      NT Suong, JC Banks, A Fidler, A Jeffs, KC Wakeman, S Webb
    • Journal Title

      Diseases of Aquatic Organisms

      Volume: 132 Issue: 3 Pages: 181-189

    • DOI

      10.3354/dao03318

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Molecular phylogeny of the benthic dinoflagellate Cabra matta (Dinophyceae) from Okinawa, Japan2018

    • Author(s)
      Yamaguchi Aika、Wakeman Kevin C.、Hoppenrath Mona、Horiguchi Takeo、Kawai Hiroshi
    • Journal Title

      Phycologia

      Volume: 57 Issue: 6 Pages: 630-640

    • DOI

      10.2216/18-7.1

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Morphology and molecular phylogeny of the marine gregarine parasite Selenidium oshoroense n. sp. (Gregarina, Apicomplexa) isolated from a Northwest Pacific Hydroides ezoensis Okuda 1934 (Serpulidae, Polychaeta)2018

    • Author(s)
      KC Wakeman, T Horiguchi
    • Journal Title

      Marine Biodiversity

      Volume: 48 Issue: 3 Pages: 1489-1498

    • DOI

      10.1007/s12526-017-0643-1

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] PCR test to specifically detect the apicomplexan ‘X’(APX) parasite found in flat oysters Ostrea chilensis in New Zealand2018

    • Author(s)
      NT Suong, JC Banks, SC Webb, A Jeffs, KC Wakeman, A Fidler
    • Journal Title

      Diseases of aquatic organisms

      Volume: 129 Issue: 3 Pages: 199-205

    • DOI

      10.3354/dao03244

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Molecular Phylogeny and Morphology of Haplozoon ezoense n. sp.(Dinophyceae): A Parasitic Dinoflagellate with Ultrastructural Evidence of Remnant Non-photosynthetic Plastids2018

    • Author(s)
      Wakeman KC, Yamaguchi A, Horiguchi T
    • Journal Title

      Protist

      Volume: 169 Issue: 3 Pages: 637-647

    • DOI

      10.1016/j.protis.2018.04.008

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Molecular Phylogenetic Positions and Ultrastructure of Marine Gregarines (Apicomplexa) Cuspisella ishikariensis n. gen., n. sp. and Loxomorpha cf. harmothoe from Western Pacific scaleworms (Polynoidae)2018

    • Author(s)
      D Iritani, T Horiguchi, KC Wakeman
    • Journal Title

      Journal of Eukaryotic Microbiology

      Volume: 65 Issue: 5 Pages: 637-647

    • DOI

      10.1111/jeu.12509

    • Related Report
      2018 Research-status Report
  • [Journal Article] Ultrastructure of the marine benthic dinoflagellate Plagiodinium belizeanum (Dinophyceae) from the southeast Pacific island of Okinawa, Japan2018

    • Author(s)
      Wakeman Kevin C.、Hoppenrath Mona、Yamaguchi Aika、Gavelis Greg S.、Leander Brian S.、Nozaki Hisayoshi
    • Journal Title

      Phycologia

      Volume: 57 Issue: 2 Pages: 209-222

    • DOI

      10.2216/17-43.1

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Molecular Phylogeny and Surface Morphology of Thiriotia hyperdolphinae n. sp. and Cephaloidophora oradareae n. sp. (Gregarinasina, Apicomplexa) Isolated from a Deep Sea Oradarea sp. (Amphipoda) in the West Pacific2017

    • Author(s)
      Wakeman Kevin C.、Yabuki Akinori、Fujikura Katsunori、Tomikawa Ko、Horiguchi Takeo
    • Journal Title

      Journal of Eukaryotic Microbiology

      Volume: - Issue: 3 Pages: 1-10

    • DOI

      10.1111/jeu.12480

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research

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Published: 2018-04-23   Modified: 2022-01-27  

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