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Phylogeny of uncultured bacteria possessing pmoA gene and rRNA approach

Research Project

Project/Area Number 18K14777
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 45030:Biodiversity and systematics-related
Research InstitutionKanazawa University

Principal Investigator

Matsuura Norihisa  金沢大学, 地球社会基盤学系, 助教 (90771585)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords16S rRNA遺伝子 / pmoA遺伝子 / メタン酸化細菌 / 遺伝子結合PCR / 機能遺伝子 / 未培養メタン酸化細菌 / バイオリアクター
Outline of Final Research Achievements

We developed an emulsion fusion PCR to link between 16S rRNA gene and functional gene. The fusion PCR was evaluated using a pure cultures experiment. And an uncultured methanotroph is discovered in forest soils as high population. We applied the developed tool to the sample and determined the phylogeny of the uncultured methanotroph.

Academic Significance and Societal Importance of the Research Achievements

温室効果ガスであるメタンは地球上の至るところから発生している。一方で、メタンを温室効果ポテンシャルの低い二酸化炭素に変換する微生物(メタン酸化細菌)も地球上に数多く存在する。このメタン酸化細菌の多くは未知微生物として知られている。本研究では、これらの微生物を検出し、その生態系を解明することを目的としている。本研究の成果は、未知微生物の生態を明らかにし、地球上の未だ不明なメタン循環の理解に貢献できる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report

Research Products

(2 results)

All 2019

All Presentation (2 results) (of which Int'l Joint Research: 2 results)

  • [Presentation] Microbial Community and Abundance in Municipal Wastewater Treatment Plant by 16S rRNA Gene Sequencing2019

    • Author(s)
      MonychotTepy Chanto, Noriko Tomioka, Kazuaki Syutsubo, Ryoko Yamamoto-Ikemoto, Norihisa Matsuura
    • Organizer
      Water and Environment technology Conference (WET) (2019)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Microbial Community Analysis in the Bioreactor Treating Methane / Ammonia Contaminated Groundwater2019

    • Author(s)
      Kazuyoshi Koike, Ryoko Yamamoto-Ikemoto, Yoshihito Nakahara, Hiroya Kodera, Norihisa Matsuura
    • Organizer
      The 8th IWA Microbial Ecology and Water Engineering Specialist Conference
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2022-01-27  

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