Identification of the initial factors that induce neural stem cell aging
| Project/Area Number |
18K14820
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 46010:Neuroscience-general-related
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| Research Institution | Kyushu University |
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Principal Investigator |
Matsuda Taito 九州大学, 医学研究院, 助教 (10756993)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 神経幹細胞 / 成体脳 / 老化 / エイジング / トランスクリプトーム解析 / 海馬 / ニューロン新生 / 加齢 / 遺伝子欠損マウス / 行動解析 / エピジェネティクス / 記憶 |
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| Outline of Final Research Achievements |
We performed RNA sequencing using EGFP-positive neural stem cells (NSCs) isolated from Nestin-EGFP mice [postnatal day (P) 5, 12w and 24w]. We have identified multiple factors that induce NSC aging. Loss of one of the identified genes, specifically in adult hippocampal NSCs, decreased NSCs and subsequent neurogenesis. Furthermore, we also found that in this gene-deficient mouse, NSCs deplete faster than usual with aging. We are now investigating whether the overexpression of this identified gene prevents the reduction of NSCs with aging.
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| Academic Significance and Societal Importance of the Research Achievements |
神経幹細胞は海馬で新しいニューロンを産生することで、学習・記憶を制御しているため、ニューロン新生能の低下は海馬組織の老化、さらには個体の老化につながることが分かっている。しかし、神経幹細胞エイジングの起点となる要因 (あるいは因子) が明らかになっていない。本研究では、幼齢期、若齢期、中齢期の各段階における神経幹細胞の遺伝子発現変化に着目し、神経幹細胞エイジングの起点と考えられる因子の同定に成功した。今後は、同定した遺伝子の発現を制御することで、神経幹細胞の機能回復、ならびに海馬学習記憶低下の改善法創出につなげたい。
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Pioneer Factor NeuroD1 Rearranges Transcriptional and Epigenetic Profiles to Execute Microglia-Neuron Conversion.2019
Author(s)
Matsuda T, Irie T, Katsurabayashi S, Hayashi Y, Nagai T, Hamazaki N, Adefuin AMD, Miura F, Ito T, Kimura H, Shirahige K, Takeda T, Iwasaki K, Imamura T, Nakashima K.
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Journal Title
Neuron
Volume: 101 (3)
Issue: 3
Pages: 472-485
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Prior Treatment with Anti-High Mobility Group Box-1 Antibody Boosts Human Neural Stem Cell Transplantation-Mediated Functional Recovery After Spinal Cord Injury.2018
Author(s)
Uezono N, Zhu Y, Y, Matsuda T,Nakajo M, Abematsu M, Setoguchi T, Mori S, Takahashi HK, Komiya S, Nishibori M, Nakashima K:
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Journal Title
Stem Cells
Volume: -
Issue: 5
Pages: 0-0
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Book] 実験医学2019
Author(s)
松田泰斗、中島欽一
Total Pages
147
Publisher
羊土社
Related Report
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