| Project/Area Number |
18K14842
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 46020:Anatomy and histopathology of nervous system-related
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | pine / Synapse / Dendrite / Parkinson's disease / Spine / FIB/SEM / interpeduncular nucleus / Postsynaptic density / Dendritic Spine / Synapses / Synaptic organisation / Neuronal circuit |
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| Outline of Final Research Achievements |
My research used three-dimensional electron microscopy to reveal the structures of neuronal compartments in different areas of the brain. I revealed the unique structures of neuronal dendrites and the placement of dendritic spines along the neuronal dendrites. Furthermore, in the striatum, I studied the developmental changes of dendritic spine morphology in 1, 3, 6 and 22 months of age in the normal mice and in the mouse model of Parkinson’s disease. I found that the spine density decreases, but the average head volume increases with age in wild-type mice. However, the increase in spine head volume with age was not seen in Parkinson’s disease mouse model. In summary, my research revealed the structure of dendritic spines in the hippocampus, cerebral cortex, striatum, cerebellum and the unique dendritic protrusion in the interpeduncular nucleus of wild-type mice; and the abnormality in the structures of dendritic spines in the striatum of Parkinson’s disease mouse model.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究で、海馬、皮質、小脳では、樹状突起の直径とともに脊椎密度が増加することが明らかにした。 さらに、正常な野生型マウスの線条体で見られた平均脊椎頭部体積の年齢による増加はパーキンソン病のモデルマウスでは観察されなかった。
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