| Project/Area Number |
18K14899
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 47030:Pharmaceutical hygiene and biochemistry-related
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 熱ストレス応答 / 核内構造体 / 長鎖非コードRNA / 非コードRNA |
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| Outline of Final Research Achievements |
We found that heat shock induces translocation of MALAT1 noncoding RNA from nuclear speckle to a distinct nuclear body named HiNoCo body. In this study, I tried to reveal the heat-response gene regulation through MALAT1/HiNoCo body. I identified the heat-responsible mRNAs and open chromatin region which were altered in MALAT1 knockout cells. We also found that MALAT1 interacted with specific Genomic DNA region in heat shock condition.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究により、MALAT1非コードRNAが熱ストレスに応答して局在変化することで、通常時と熱ストレス時では異なる遺伝子発現の制御に関与することが示唆された。本研究は「非コードRNAの局在変化を基盤とした熱応答性遺伝子の発現制御」という新しい熱ストレス応答機構の発見につながる、先駆的な事例となることが期待される。
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