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Genome-wide screening for host factors involved in Japanese encephalitis virus infection

Research Project

Project/Area Number 18K14912
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 47030:Pharmaceutical hygiene and biochemistry-related
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

Sakuma Chisato  国立感染症研究所, 細菌第一部, 主任研究官 (80782888)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2020: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords日本脳炎ウイルス / ゲノムワイドスクリーニング / 宿主因子 / Huh7細胞 / ゲノムワイド探索
Outline of Final Research Achievements

Japanese encephalitis virus (JEV) is widely distributed to Asia, causing serious acute encephalitis. Although the mechanism of anti-JEV responses have been investigated by various studies, much remains unknown.
The aim of this study was to identify host factors involving to resistance to JEV infection by gene knockout screen using CRISPR/Cas9 system or gene activation screen using CRISPR/dCAS9 system. As a result of two genome wide screens, multiple host factors involved in JEV infection were identified.

Academic Significance and Societal Importance of the Research Achievements

日本脳炎は東アジア、東南アジア、南アジアにかけて各地に広く流行している急性脳炎である。日本国内ではワクチンの接種により流行は制御されているが、ブタが同ウイルスを有しているため感染の機会はなくなっていない。
本研究では遺伝子ノックアウト系スクリーニングにより日本脳炎ウイルスのライフサイクルに必要な宿主因子を単離し、遺伝子発現活性化系スクリーニングにより同ウイルスに抵抗性を示す宿主因子の単離に成功した。日本脳炎ウイルスのライフサイクルや宿主の抗ウイルス応答機構について新たな知見を得ることができ、感染症に関する基礎研究や感染症対策において非常に意義があった。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (1 results)

All 2021

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results)

  • [Journal Article] Identification of SYS1 as a Host Factor Required for Shiga Toxin-Mediated Cytotoxicity in Vero Cells2021

    • Author(s)
      Sakuma Chisato、Sekizuka Tsuyoshi、Kuroda Makoto、Hanada Kentaro、Yamaji Toshiyuki
    • Journal Title

      Int J Mol Sci

      Volume: 22 Issue: 9 Pages: 4936-4936

    • DOI

      10.3390/ijms22094936

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access

URL: 

Published: 2018-04-23   Modified: 2023-01-30  

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