| Project/Area Number |
18K15005
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 48010:Anatomy-related
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| Research Institution | Niigata University (2019)
Kobe University (2018) |
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Principal Investigator |
Liu Xinyi 新潟大学, 脳研究所, 研究機関研究員 (40813928)
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| Project Period (FY) |
2018-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | lymphatic / development / mouse / Development / Lymphatic vessel / Mouse / Platelet |
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| Outline of Final Research Achievements |
Lymphatic vessels are established as a vasculature separate from blood vessels in peripheral tissues.In this study, we analyzed Phospholipase Cγ2 (Plcg2) knockout mice, which lack platelet activation and show blood-filled lymphatic vasculature. We detected lymph-blood misconnections in embryonic back skin of Plcg2 knockout mice by fluorescent angiography, indicating the role of platelets in the maintenance of lymph-blood partitioning during lymphatic vessel elongation. We also performed time-lapse analysis and found a retraction of LEC protrusion in vitro. These effects were inhibited by an inhibitor of TGF-β signals, while TGF-β induced a retraction of LEC protrusion. In vivo analysis indicates the role of TGF-β in keeping lymphatic vessels separate from blood vessels. These results indicate a novel role for platelets in partitioning blood and lymphatic vascular compartments by promoting LEC retraction in mouse peripheral tissues.
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| Academic Significance and Societal Importance of the Research Achievements |
We confirmed platelets function to keep lymph-blood partitioning in mouse peripheral tissues after LECs migrate out of the jugular veins. It indicate a novel role for platelets in partitioning blood and lymphatic vascular compartments by promoting LEC retraction in mouse peripheral tissues.
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