Overview of the regulation of skeletal muscle metabolism based on comprehensive molecular quantification
| Project/Area Number |
18K15011
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 48020:Physiology-related
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 骨格筋 / インスリン / マルチオミクス / 代謝 / プロテオミクス / 微分方程式モデル |
|---|
| Outline of Final Research Achievements |
In this project, skeletal muscle composition was analyzed in terms of molecular concentration/proportion, and their temporal variation over four hours after insulin administration. A molecular interaction network consisting of approximately 10,000 insulin-responsive molecules (nodes) was constructed based on this analysis. This network illustrates about 20,000 interactions (edges) among metabolites, RNAs, and proteins that changed in their phosphorylation or expression levels. Such a comprehensive analysis provided an overview of major regulatory processes within skeletal muscles in response to insulin. Our investigation model enabled the design of scalable methods that could integrate multiple sources to characterize and visualize biologically relevant molecular interactions.
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| Academic Significance and Societal Importance of the Research Achievements |
網羅的な分子定量から分子間相互作用ネットワークの構築・特徴解析・可視化までのワークフローの開発により、大規模データと既存知識を統合して生物組織において誘導される分子制御の全体像の理解が可能になった。このアプローチは拡張性が高いことから、適用範囲は骨格筋代謝に限らない。今後、より幅広い生命現象の制御メカニズム理解への応用が期待される。
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Report
(6 results)
Research Products
(17 results)
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[Presentation] Trans-omic analysis of insulin response in the liver2022
Author(s)
Fumiko Matsuzaki, Shinsuke Uda, Yukiyo Yamauchi, Masaki Matsumoto, Tomoyoshi Soga, Kazumitsu Maehara, Yasuyuki Ohkawa, Keiichi I. Nakayama, Shinya Kuroda, Hiroyuki Kubota
Organizer
The 31st Hot Spring Harbor International Symposium: Expanding views on Systems biology and Immunology
Related Report
Int'l Joint Research
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[Presentation] トランスオミックネットワーク作成による分子制御機構の可視化2022
Author(s)
松崎芙美子, 湯通堂紀子, 山内幸代, 宇田新介, 和泉自泰, 高橋政友, 中尾素直, 油屋駿介, 馬場健史, 曽我朋義, 菊竹智恵, 須山幹太, 原田哲仁, 前原一満, 大川恭行, 松本雅記, 中山敬一, 黒田真也, 久保田浩行
Organizer
第45回日本分子生物学会年会
Related Report
Invited
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[Presentation] 時系列トランスオミクス解析2020
Author(s)
松崎芙美子, 宇田新介, 山内幸代, 松本雅記, 曽我朋義, 前原一満, 大川恭行, 中山敬一, 黒田真也, 久保田浩行
Organizer
IIBMP2020（2020年日本バイオインフォマティクス学会年会 第9回生命医薬情報学連合大会）
Related Report
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[Presentation] Integrated analysis through multiple molecular layers: transomic network dynamics2020
Author(s)
Fumiko Matsuzaki, Shinsuke Uda, Yukiyo Yamauchi, Masaki Matsumoto, Tomoyoshi Soga, Kazumitsu Maehara, Yasuyuki Ohkawa, Keiichi I. Nakayama, Shinya Kuroda, Hiroyuki Kubota
Organizer
The 29th Hot Spring Harbor International Symposium, Cutting Edge of Technical Innovations in Trans-Omics
Related Report
Int'l Joint Research
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[Presentation] TRANSOMICS: Integrated Analysis Through Multiple Molecular Layers2018
Author(s)
Fumiko Matsuzaki, Shinsuke Uda, Yukiyo Yamauchi, Kazumitsu Maehara, Yasuyuki Ohkawa, Masaki Matsumoto, Keiichi I. Nakayama, Hiroyuki Kubota
Organizer
The Joint Symposium of the 13th International Symposium of the Institute Network for Biomedical Sciences and the 28th Hot Spring Harbor International Symposium 2018
Related Report
Int'l Joint Research
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