Dissecting molecular function of JAM family protein in adipose derived stromal cells

• Project/Area Number: 18K15127
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 49030:Experimental pathology-related
• Research Institution: Fukushima Medical University
• Principal Investigator: Kaneko Tetsuharu 福島県立医科大学, 医学部, 講師 (20598255)
• Project Period (FY): 2018-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2019: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 細胞間接着 / 間葉系幹細胞 / 培養補助技術 / JAM / 幹細胞培養 / 細胞接着 / 幹細胞 / 再生医学

Outline of Final Research Achievements

The JAM family is expressed not only in epithelial cells but also in a variety of stem cells, whereas their functions remain obscure. We analyzed the expression and function of JAMs in adipose-derived stem cells (ADSCs). Among the eight members of JAMs, JAM-B and JAM-C were expressed on surface of ADSCs. Among them, JAM-C was broadly distributed on the extracellular matrix of adipose tissue after cleavage of the extracellular domain. By coating the culture dishes with the soluble JAM-C, the proliferation and stemness of ADSCs were both enhanced, indicating its utility as stem cell culture-material.

Academic Significance and Societal Importance of the Research Achievements

幹細胞を用いた細胞治療には極めて大量の細胞を確保する必要があるが、幹細胞は特殊な培養条件を必要とすることもあり莫大な費用がかかる。よって再生医療の実現にあたって効率の改善と低コスト化が望まれている。本研究により、JAM-C可溶体の幹細胞培養基剤としての有用性が示された。JAM-Cは分子量が小さくリコンビナント蛋白質として比較的安価に調整することができることから、新規幹細胞培養補助剤として有望であると考えられた。

Research Products

• [Journal Article] Evaluation of a Newly Designed Microperforated Pure Titanium Membrane for Guided Bone Regeneration. (2019)
  • Author(s): Hasegawa H, Masui S, Ishihata H, Kaneko T, Ishida D, Endo M, Kanno C, Yamazaki M, Kitabatake T, Utsunomiya S, Izumi K, Sasaki K.
  • Journal Title: Int J Oral Maxillofac Implants Volume: 34 Issue: 2 Pages: 411-422
  • DOI: 10.11607/jomi.6777
  • Peer Reviewed
• [Journal Article] Evaluation of a Newly Designed Microperforated Titanium Membrane with Beta-Tricalcium Phosphate for Guided Bone Regeneration in Dog Mandibles (2019)
  • Author(s): Hasegawa Hiroshi、Kaneko Tetsuharu、Kanno Chihiro、Endo Manabu、Akimoto Tetsuo、Yamazaki Morio、Kitabatake Takehiro、Masui Seiichiro、Ishihata Hiroshi、Izumi Kenji
  • Journal Title: The International Journal of Oral & Maxillofacial Implants Volume: 34 Issue: 5 Pages: 1132-1142
  • DOI: 10.11607/jomi.6776
  • Peer Reviewed