Characterization of novel paramyxoviruses isolated from bats in Africa

• Project/Area Number: 18K15163
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 49060:Virology-related
• Research Institution: Hokkaido University
• Principal Investigator: Kajihara Masahiro 北海道大学, 人獣共通感染症リサーチセンター, 助教 (70711894)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: パラミクソウイルス / コウモリ / ザンビア / アフリカ / ムンプスウイルス / 人獣共通感染症 / ブニヤウイルス / ムンプス / ナイロウイルス / バンヤンウイルス

Outline of Final Research Achievements

Bat mumps virus (bMuV), a novel paramyxovirus, was isolated from fruit bats in Zambia. Full-genome sequence analyses revealed bMuV was closely related to human mumps virus and they have 77% homology in nucleotide sequences. Genetic screening showed that 21% of the bats examined were positive for bMuV genome and the virus was frequently detected in spleens, kidneys, colons, and salivary glands. The findings suggested that bMuV may be transmitted through bat secreta such as saliva, urine, and feces. Mice, rats, hamsters, and guinea pigs challenged with bMuV through either of intranasally or intraperitoneal route did not show any apparent clinical signs. However, induction of bMuV-specific antibodies and viral growth in tissues, such as spleens, salivary glands, and colons, were observed, indicating that bMuV has a potential to infect mammalian hosts other than bats.

Academic Significance and Societal Importance of the Research Achievements

COVID-19の例からも明らかなように、コウモリ由来ウイルスによる新興感染症は地球規模の脅威となりうる。新興感染症の発生を防ぐには、動物からヒトへの病原体の伝播を防ぐことが効果的である。本研究を通してbMuVのコウモリにおける伝播様式、生存様式の一端が明らかとなった。ウイルスの生態に関する理解は、コウモリ由来ウイルスによる新興感染症に対する先回り対策を講じる上で知識的基盤となる。また、bMuVの齧歯類動物に対する感染性が示された。ムンプスウイルス感染症の動物モデルはこれまでに確立されていない。本ウイルスがムンプスウイルスの感染様式を明らかにする上での代替モデルになりうる可能性が示唆された。

Research Products

• [Int'l Joint Research] ザンビア大学獣医学部/大学教育病院/国立公園・野生動物局(ザンビア)
• [Int'l Joint Research] ザンビア大学獣医学部(ザンビア)
• [Int'l Joint Research] ザンビア大学 獣医学部/国立公園・野生動物管理局(ザンビア)
• [Journal Article] 特集 新型ウイルスの最新情報と対応 新たな問題となりうるコウモリ･節足動物媒介性ウイルス (2021)
  • Author(s): 梶原将大, 大場靖子, 高田礼人
  • Journal Title: Pharma Medica Volume: 39 Issue: 1 Pages: 43-47
  • DOI: 10.34449/J0001.39.01_0043-0047
  • ISSN: 0289-5803
  • Year and Date: 2021-01-20
• [Journal Article] First COVID-19 case in Zambia ? Comparative phylogenomic analyses of SARS-CoV-2 detected in African countries (2021)
  • Author(s): Simulundu E, Mupeta F, Chanda-Kapata P, Saasa N, Changula K, Muleya W, Chitanga S, Mwanza M, Simusika P, Chambaro H, Mubemba B, Kajihara M, Chanda D, Mulenga L, Fwoloshi S, Shibemba AL, Kapaya F, Zulu P, Musonda K, Monze M, Sinyange N, Mazaba ML, Kapin’a M, Chipimo PJ, Hamoonga R, Simwaba D, Ngosa W, et al.
  • Journal Title: International Journal of Infectious Diseases Volume: 102 Pages: 455-459
  • DOI: 10.1016/j.ijid.2020.09.1480
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Prevalence and genetic diversity of Shibuyunji virus, a novel tick-borne phlebovirus identified in Zambia (2021)
  • Author(s): Simulundu E, Mbambara S, Chambaro HM, Sichibalo K, Kajihara M, Nalubamba KS, Sawa H, Takada A, Changula K, Chitanga S
  • Journal Title: Archives of Virology Volume: 166 Issue: 3 Pages: 915-919
  • DOI: 10.1007/s00705-020-04924-9
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Purification of Crimean?Congo hemorrhagic fever virus nucleoprotein and its utility for serological diagnosis (2021)
  • Author(s): Lombe BP, Miyamoto H, Saito T, Yoshida R, Manzoor R, Kajihara M, Shimojima M, Fukushi S, Morikawa S, Yoshikawa T, Kurosu T, Saijo M, Tang Q, Masumu J, Hawman D, Feldmann H, Takada A
  • Journal Title: Scientific Reports Volume: 11 Issue: 1 Pages: 2324-2324
  • DOI: 10.1038/s41598-021-81752-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Detection of B.1.351 SARS-CoV-2 Variant Strain ? Zambia, December 2020 (2021)
  • Author(s): Mwenda M, Saasa N, Sinyange N, Busby G, Chipimo PJ, Hendry J, Kapona O, Yingst S, Hines JZ, Minchella P, Simulundu E, Changula K, Nalubamba KS, Sawa H, Kajihara M, Yamagishi J, Kapin’a M, Kapata N, Fwoloshi S, Zulu P, Mulenga LB, Agolory S, Mukonka V, Bridges DJ
  • Journal Title: MMWR. Morbidity and Mortality Weekly Report Volume: 70 Issue: 8 Pages: 280-282
  • DOI: 10.15585/mmwr.mm7008e2
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An African tick flavivirus forming an independent clade exhibits unique exoribonuclease-resistant RNA structures in the genomic 3′-untranslated region (2021)
  • Author(s): Harima Hayato、Orba Yasuko、Torii Shiho、Qiu Yongjin、Kajihara Masahiro、Eto Yoshiki、Matsuta Naoya、Hang’ombe Bernard M.、Eshita Yuki、Uemura Kentaro、Matsuno Keita、Sasaki Michihito、Yoshii Kentaro、Nakao Ryo、Hall William W.、Takada Ayato、Abe Takashi、Wolfinger Michael T.、Simuunza Martin、Sawa Hirofumi
  • Journal Title: Scientific Reports Volume: 11 Issue: 1 Pages: 4883-4883
  • DOI: 10.1038/s41598-021-84365-9
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A potential role of non-neutralizing IgA antibodies in cross-protective immunity against influenza A viruses of multiple hemagglutinin subtypes (2020)
  • Author(s): Okuya K, Yoshida R, Manzoor R, Saito S, Suzuki T, Sasaki M, Saito T, Kida Y, Mori-Kajihara A, Kondoh T, Sato M, Kajihara M, Miyamoto H, Ichii O, Higashi H, Takada A
  • Journal Title: J Virol Volume: - Issue: 12 Pages: 408-420
  • DOI: 10.1128/jvi.00408-20
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Evidence for exposure of asymptomatic domestic pigs to African Swine fever virus during an inter-epidemic period in Zambia. (2020)
  • Author(s): Chambaro HM, Sasaki M, Sinkala Y, Gonzalez G, Squarre D, Fandamu P, Lubaba C, Mataa L, Shawa M, Mwape KE, Gabri_ S, Chembensofu M, Carr MJ, Hall WW, Qiu Y, Kajihara M, TakadaA, Orba Y, Simulundu E, Sawa H
  • Journal Title: Transbound Emerg Dis Volume: 67(6) Issue: 6 Pages: 2741-2752
  • DOI: 10.1111/tbed.13630
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Isolation of Candidatus Bartonella rousetti and Other Bat-associated Bartonellae from Bats and Their Flies in Zambia (2020)
  • Author(s): Qiu Yongjin、Kajihara Masahiro、Nakao Ryo、Mulenga Evans、Harima Hayato、Hang’ombe Bernard Mudenda、Eto Yoshiki、Changula Katendi、Mwizabi Daniel、Sawa Hirofumi、Higashi Hideaki、Mweene Aaron、Takada Ayato、Simuunza Martin、Sugimoto Chihiro
  • Journal Title: Pathogens Volume: 9 Issue: 6 Pages: 469-469
  • DOI: 10.3390/pathogens9060469
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Comparative Analyses of the Antiviral Activities of IgG and IgA Antibodies to Influenza A Virus M2 Protein (2020)
  • Author(s): Okuya Kosuke、Eguchi Nao、Manzoor Rashid、Yoshida Reiko、Saito Shinji、Suzuki Tadaki、Sasaki Michihito、Saito Takeshi、Kida Yurie、Mori-Kajihara Akina、Miyamoto Hiroko、Ichii Osamu、Kajihara Masahiro、Higashi Hideaki、Takada Ayato
  • Journal Title: Viruses Volume: 12 Issue: 7 Pages: 780-780
  • DOI: 10.3390/v12070780
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Characterization of mammalian orthoreoviruses isolated from faeces of pigs in Zambia. (2020)
  • Author(s): Harima H, Sasaki M, Kajihara M, Gonzalez G, Simulundu E, Bwalya EC, Qiu Y,Okuya K, Isono M, Orba Y, Takada A, Hang'ombe BM, Mweene AS, Sawa H.
  • Journal Title: J Gen Virol Volume: 101(10) Issue: 10 Pages: 1027-1036
  • DOI: 10.1099/jgv.0.001476
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Avian Influenza Viruses Detected in Birds in Sub-Saharan Africa: A Systematic Review (2020)
  • Author(s): Kalonda A, Saasa N, Nkhoma P, Kajihara M, Sawa H, Takada A, Simulundu E
  • Journal Title: Viruses Volume: 12 Issue: 9 Pages: 993-993
  • DOI: 10.3390/v12090993
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A biaryl sulfonamide derivative as a novel inhibitor of filovirus infection. (2020)
  • Author(s): Isono M, Furuyama W, Kuroda M, Kondoh T, Igarashi M, Kajihara M, Yoshida R, Manzoor R, Okuya K, Miyamoto H, Feldmann H, Marzi A, Sakaitani M, Nanbo A, Takada A
  • Journal Title: Antiviral Res Volume: 183 Pages: 104932-104932
  • DOI: 10.1016/j.antiviral.2020.104932
  • NAID: 120007173916
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Receptor-Mediated Host Cell Preference of a Bat-Derived Filovirus, Lloviu Virus (2020)
  • Author(s): Takadate Yoshihiro、Manzoor Rashid、Saito Takeshi、Kida Yurie、Maruyama Junki、Kondoh Tatsunari、Miyamoto Hiroko、Ogawa Hirohito、Kajihara Masahiro、Igarashi Manabu、Takada Ayato
  • Journal Title: Microorganisms Volume: 8 Issue: 10 Pages: 1530-1530
  • DOI: 10.3390/microorganisms8101530
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Genetic and Biological Diversity of Porcine Sapeloviruses Prevailing in Zambia (2020)
  • Author(s): Harima H, Kajihara M, Simulundu E, Bwalya E, Qiu Y, Isono M, Okuya K, Gonzalez G, Yamagishi J, Hang'ombe BM, Sawa H, Mweene AS, Takada A
  • Journal Title: Viruses Volume: 12(2) Issue: 2 Pages: 180-180
  • DOI: 10.3390/v12020180
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] West Nile Virus in Farmed Crocodiles, Zambia, 2019 (2020)
  • Author(s): Simulundu E, Ndashe K, Chambaro MC, Squarre D, Reilly MP, Chitanga S, Changula K, Mukubesa NA, Ndebe J, Tembo J, Kapata N, Bates M, Sinkala Y, Hang’ombe MB, Nalubamba SK, Kajihara M, Sasaki M, Orba Y, Takada A, Sawa H
  • Journal Title: Emerg. Infect. Dis. Volume: 26(4) Issue: 4 Pages: 811-814
  • DOI: 10.3201/eid2604.190954
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Characterization of non-typhoid Salmonellae isolated from domestic animals and wildlife from selected areas of Zambia (2020)
  • Author(s): Mubita MC, Muma JB, Samui K, Saasa N, Simulundu E, Ndebe J, Kajihara M, Qiu Y, Hang’ombe MB
  • Journal Title: Scientific African Volume: 8 Pages: e00345-e00345
  • DOI: 10.1016/j.sciaf.2020.e00345
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Seroprevalence of Rift Valley fever in cattle of smallholder farmers in Kwilu Province in the Democratic Republic of Congo (2019)
  • Author(s): Halawi AD, Saasa N, Pongombo BL, Kajihara M, Chambaro HM, Hity M, Sawa H, Takada A, Mweene AS, Nsembo LL, Simulundu E
  • Journal Title: Trop. Anim. Health Prod. Volume: 51(8) Issue: 8 Pages: 2619-2627
  • DOI: 10.1007/s11250-019-01978-z
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Genetic diversity of rabies virus in different host species and geographic regions of Zambia and Zimbabwe (2019)
  • Author(s): Muleya W, Chambaro HM, Sasaki M, Gwenhure LF, Mwenechanya R, Kajihara M, Saasa N, Mupila Z, Mori-Kajihara A, Qiu Y, Kangwa E, Mweene A, Namangala B, Takada A, Sawa H
  • Journal Title: Virus Genes Volume: 55(5) Issue: 5 Pages: 713-719
  • DOI: 10.1007/s11262-019-01682-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular detection and characterization of genotype 1 bovine leukemia virus from beef cattle in the traditional sector in Zambia (2019)
  • Author(s): Phiri MM, Kaimoyo E, Changula K, Silwamba I, Chambaro HM, Kapila P, Kajihara M, Simuunza M, Muma JB, Pandey GS, Takada A, Mweene AS, Chitanga S, Simulundu E
  • Journal Title: Arch. Virol. Volume: 164(10) Issue: 10 Pages: 2531-2536
  • DOI: 10.1007/s00705-019-04350-6
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Generation of bat-derived influenza viruses and their reassortants (2019)
  • Author(s): Sato Masahiro、Maruyama Junki、Kondoh Tatsunari、Nao Naganori、Miyamoto Hiroko、Takadate Yoshihiro、Furuyama Wakako、Kajihara Masahiro、Ogawa Hirohito、Manzoor Rashid、Yoshida Reiko、Igarashi Manabu、Takada Ayato
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 1158-1158
  • DOI: 10.1038/s41598-018-37830-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Monoclonal Antibody Cocktail Protects Hamsters From Lethal Marburg Virus Infection (2018)
  • Author(s): Marzi A, Haddock E, Kajihara M, Feldmann H, Takada A
  • Journal Title: The Journal of Infectious Diseases Volume: 218 Issue: suppl_5 Pages: S662-S665
  • DOI: 10.1093/infdis/jiy235
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Identification of group A rotaviruses from Zambian fruit bats provides evidence for long-distance dispersal events in Africa (2018)
  • Author(s): Sasaki M, Kajihara M, Changula K, Mori-Kajihara A, Ogawa H, Hang'ombe B M, Mweene A S, Simuunza M, Yoshida R, Carr M, Orba Y, Takada A, Sawa H
  • Journal Title: Infection, Genetics and Evolution Volume: 63 Pages: 104-109
  • DOI: 10.1016/j.meegid.2018.05.016
  • NAID: 120006716313
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] normal Expression of a Recombinant Nucleocapsid Protein of Rift Valley Fever Virus in Vero Cells as an Immunofluorescence Antigen and Its Use for Serosurveillance in Traditional Cattle Herds in Zambia (2018)
  • Author(s): Saasa Ngonda、Kajihara Masahiro、Dautu George、Mori-Kajihara Akina、Fukushi Shuetsu、Sinkala Yona、Morikawa Shigeru、Mweene Aaron、Takada Ayato、Yoshimatsu Kumiko、Arikawa Jiro
  • Journal Title: Vector-Borne and Zoonotic Diseases Volume: 印刷中 Issue: 5 Pages: 273-277
  • DOI: 10.1089/vbz.2017.2186
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Discovery of Mwinilunga alphavirus: a novel alphavirus (2018)
  • Author(s): Torii S, Orba Y, Hang'ombe BM, Mweene AS, Wada Y, Anindita PD, Phongphaew W,
  • Journal Title: Virus Research Volume: 18 Pages: 31-36
  • DOI: 10.1016/j.virusres.2018.04.005
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] アフリカにおけるウイルス探索 (2020)
  • Author(s): 梶原 将大
  • Organizer: 金沢大学グローバルサイエンスキャンパス事業 国際ゼミ
  • Invited
• [Presentation] アフリカでのウイルスハンティング -出血熱ウイルスから未知のウイルスまで- (2020)
  • Author(s): 梶原 将大
  • Organizer: 第19回みちのくウイルス塾
  • Invited
• [Presentation] ボレリア感染症におけるコウモリの生態学的役割に関する研究. (2020)
  • Author(s): 梶原 将大
  • Organizer: “回帰熱を主とした節足動物媒介性感染症に関する総合的対策のための研究” キックオフシンポジウム
  • Invited
• [Presentation] Seroprevalence of Filovirus Infection in Rousettus aegyptiacus Bats in Zambia (2019)
  • Author(s): Kajihara M, Changula K, Hang’ombe B, Mori-Kajihara A, Eto Y, Miyamoto H, Qiu Y, Harima H, Yoshida R, Shigeno A, Mwizabi D, Squarre D, Orba Y, Sawa H, Ogawa H, Simulundu E, Mukonka V, Mweene A, Takada A
  • Organizer: 18th International Bat Research Conference
  • Int'l Joint Research
• [Presentation] Marburgvirus in Egyptian fruit bats in Zambia (2019)
  • Author(s): Kajihara M
  • Organizer: 感染症研究国際展開戦略プログラム（J-GRID）新興・再興感染症に関するアジア・アフリカ リサーチフォーラム2019
  • Int'l Joint Research
• [Presentation] ザンビアのエジプトルーセットオオコウモリから検出されたマールブルグウイルスの遺伝学的解析 (2019)
  • Author(s): Kajihara M, Changula M, Hang’ombe B, Harima H, Miyamoto H, Eto Y, Okuya K, Isono M, Yoshida R, Qiu Y, Mori-Kajihara A, Orba Y, Sawa H, Ogawa H, Squarre D, Mukonka V, Mweene A, Takada A
  • Organizer: 第67回 日本ウイルス学会学術集会
• [Presentation] エボラウイルスの細胞侵入阻害薬の探索 (2019)
  • Author(s): Isono M, Furuyama W, Kuroda M, Kondo T, Igarashi M, Kajihara M, Yoshida R, Manzoor R, Okuya K, Miyamoto H, Feldmann H, Marzi A, Sakaitani M, Takada A
  • Organizer: 第67回 日本ウイルス学会学術集会
• [Presentation] ザンビアのダニで発見した新規フラビウイルスはフラビウイルス属の中で独特な系統を形成するNgoye virusに遺伝的に密接に関係している (2019)
  • Author(s): Harima H, Torii S, Qiu Y, Kajihara M, Eto Y, Orba Y, Eshita Y, Hang’ombe B, Yoshii K, Martin S, Takada A, Sawa H
  • Organizer: 第67回 日本ウイルス学会学術集会
• [Presentation] ザンビアのコウモリにおけるウイルス保有調査 (2019)
  • Author(s): 梶原 将大、Changula K、宮本 洋子、邱 永晋、播磨 勇人、Hang’ombe B M、Mweene A、高田 礼人
  • Organizer: 8th Negative Strand Virus-Japan Symposium
• [Presentation] First isolation and phylogenetic characterization of Sapelovirus from piglets in Africa (2018)
  • Author(s): Harima H, Kajihara M, Simulundu E, Bwalya E, Ndebe J, Qiu Y, Hang'ombe B M, Aaron M S, Takada A
  • Organizer: The 66th Annual Meeting of the Japanese Society for Virology
• [Presentation] Identification of unknown viruses from wildlife in Zambia (2018)
  • Author(s): Kajihara M
  • Organizer: Studium Generale 3 Disease Control of the Wildlife-Livestock-Human interface: Series
  • Invited
• [Book] ザンビアを知るための55章 (2020)
  • Author(s): 島田周平, 大山修一, 荒木茂, 石本雄大, 伊藤千尋, 梅津千恵子, 遠藤貢, 興津妙子, 奥村正裕, 小倉充夫, 梶原将大, 川畑一朗, 姜明江, 児玉谷史朗, 真常仁志, 杉山祐子, 大門碧, 髙田礼人, 高橋基樹, 立山由紀子, ら.
  • Total Pages: 376
  • Publisher: 明石書店
  • ISBN: 9784750350622