| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Outline of Final Research Achievements |
We have identified novel adaptive mutations in humans by comprehensively searching for adaptive mutations acquired by avian influenza virus in infected patients. The purpose of this study was to identify host factors essential for viral replication using the mutant viruses as human adaptation model viruses.
We established an immunoprecipitation method that efficiently coprecipitates host factors that bind to polymerase complex, and selected multiple host factors that specifically bind to mutants by LC-MS / MS. We evaluated the effect of this factor on viral replication, but the factors that affect viral replication have not been identified. We are trying to select candidate factors more widely and create multiple knockdown cells.
|
