Analysis of interactions between virus and host factors regarding antigen presentation

• Project/Area Number: 18K15175
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 49060:Virology-related
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: Seki Sayuri 国立感染症研究所, エイズ研究センター, 主任研究官 (70758325)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ヒト免疫不全ウイルス / サル免疫不全ウイルス / 細胞傷害性T細胞 / 抗原提示 / 宿主因子 / HIV / MHC-I / ウイルスタンパク

Outline of Final Research Achievements

Induction of virus-specific cytotoxic T lymphocyte (CTL) responses has been shown to be important in controlling HIV infection. Optimization of antigens is necessary to induce virus-specific CTLs effectively. In the present study, target cells expressing Vif were killed by CTLs efficiently in the presence of Vif-APOBEC3G interaction. These results gave molecular level insight into reasonable design of HIV antigens.

Academic Significance and Societal Importance of the Research Achievements

本研究においては、宿主因子と相互作用を有するウイルス蛋白質が、固有のメカニズムによってエイズウイルス複製制御に結びつく有効なCTL抗原となりうる可能性が新しく見出された。宿主因子・ウイルス蛋白質・CTL反応それぞれの機構が繋がった学術的意義は大きい。また、本研究はHIV感染症の流行を抑えるための免疫反応誘導戦略を考える上で、より有効な抗原をデザインするための理論基盤の一部をなすため、社会的意義のある成果である。

Research Products

• [Journal Article] Fecal Microbiome Composition in Healthy Adults in Ghana (2021)
  • Author(s): Parbie Prince Kofi、Mizutani Taketoshi、Ishizaka Aya、Kawana-Tachikawa Ai、Runtuwene Lucky Ronald、Seki Sayuri、Abana Christopher Zaab-Yen、Kushitor Dennis、Bonney Evelyn Yayra、Ofori Sampson Badu、Uematsu Satoshi、Imoto Seiya、Kimura Yasumasa、Kiyono Hiroshi、Ishikawa Koichi、Ampofo William Kwabena、Matano Tetsuro
  • Journal Title: Japanese Journal of Infectious Diseases Volume: 74 Issue: 1 Pages: 42-47
  • DOI: 10.7883/yoken.JJID.2020.469
  • NAID: 130007973724
  • ISSN: 1344-6304, 1884-2836
  • Year and Date: 2021-01-29
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Therapeutic vaccine-mediated Gag-specific CD8+ T-cell induction under anti-retroviral therapy augments anti-virus efficacy of CD8+ cells in simian immunodeficiency virus-infected macaques (2020)
  • Author(s): Nakamura-Hoshi Midori、Takahara Yusuke、Matsuoka Saori、Ishii Hiroshi、Seki Sayuri、Nomura Takushi、Yamamoto Hiroyuki、Sakawaki Hiromi、Miura Tomoyuki、Tokusumi Tsuyoshi、Shu Tsugumine、Matano Tetsuro
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 11394-11394
  • DOI: 10.1038/s41598-020-68267-w
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] CD8+ T cell-based strong selective pressure on multiple simian immunodeficiency virus targets in macaques possessing a protective MHC class I haplotype (2019)
  • Author(s): Hau Trang Thi Thu、Nakamura-Hoshi Midori、Kanno Yoshiaki、Nomura Takushi、Nishizawa Masako、Seki Sayuri、Ishii Hiroshi、Kawana-Tachikawa Ai、Hall William W.、Nguyen Thi Lan Anh、Matano Tetsuro、Yamamoto Hiroyuki
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 512 Issue: 2 Pages: 213-217
  • DOI: 10.1016/j.bbrc.2019.03.003
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Vifの抗原提示に関する研究 (2020)
  • Author(s): 関紗由里，岩谷靖雅，石井洋，俣野哲朗
  • Organizer: 第34回日本エイズ学会学術集会
• [Presentation] Fecal bacterial profile in HIV associated gut microbial dysbiosis (2019)
  • Author(s): Prince Kofi Parbie, Taketoshi Mizutani, Aya Ishizaka, Sayuri Seki, Christopher Zaab-Yen Abana, Dennis Kushitor, Evelyn Yayra Bonney, Sampson Badu Ofori, William Kwabena Ampofo, Koichi Ishikawa, Tetsuro Matano
  • Organizer: 第67回日本ウイルス学会学術集会
• [Presentation] Microbial dysbiosis and associated functional shifts in gut microbiome of HIV infected patients (2019)
  • Author(s): Prince Kofi Parbie, Taketoshi Mizutani, Aya Ishizaka, Sayuri Seki, Christopher Zaab-Yen Abana, Dennis Kushitor, Evelyn Yayra Bonney, Sampson Badu Ofori, William Kwabena Ampofo, Koichi Ishikawa, Tetsuro Matano
  • Organizer: 第33回日本エイズ学会学術集会
• [Presentation] Protective and non-protective MHC-I haplotype-associated CD8+ T-cell responses in simian immunodeficiency virus-infected rhesus macaques (2018)
  • Author(s): Hau Thi Thu Trang, Takushi Nomura, Sayuri Seki, Midori Nakamura-Hoshi, Hiroshi Ishii, Taeko K. Naruse, Akinori Kimura, Tetsuro Matano, Hiroyuki Yamamoto
  • Organizer: 第66回ウイルス学会学術集会