| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The muscle disease is hard to treat and the development of a new medicine is necessary to improve muscle function. The phosphorylation of myosin regulatory light chain by skeletal muscle myosin light chain kinase (skMLCK) is important for muscle contraction. To activate or inhibit skMLCK may induce to increase or reduce the force produced by skeletal muscle, respectively.
In our present study, we performed the high throughput screening for skMLCK activator or inhibitor using Tokyo university low molecular weight compounds full library. And we finally found the 106 candidates of activator and 12 candidates of inhibitor. Furthermore, we constructed the measurement system of muscle force, and successfully showed that the skMLCK gene transfer using adeno associated vector induced the increment of muscle force in SOD mouse (which is the model mouse of amyotrophic lateral sclerosis).
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