Usefulness of cell-free DNA in patients with oligometastatic cancer

• Project/Area Number: 18K15611
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 52040:Radiological sciences-related
• Research Institution: National Cancer Center Japan
• Principal Investigator: Nakamura Masaki 国立研究開発法人国立がん研究センター, 東病院, 医員 (90804287)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: オリゴメタスタシス / 放射線治療 / リキッドバイオプシー / cfDNA / 大腸癌 / 大腸直腸癌 / 無増悪生存率 / 直腸癌 / cell free DNA / Cell free DNA

Outline of Final Research Achievements

We detected and analyzed tumor-derived DNA from the blood of patients who had undergone radiotherapy for a small number of metastases in colorectal cancer before radiotherapy. As a result, it was suggested that the tumor-derived DNA could be detected, and that the tumor-derived DNA could be used to determine the likelihood of recurrence after treatment. We have obtained important data for further verification in the future.

Academic Significance and Societal Importance of the Research Achievements

大腸癌、直腸癌で少数個の転移に対して放射線治療を行う患者さんの放射線治療前の血液検査で再発しやすさがわかれば、効果が乏しい患者さんへの不要な治療を事前に回避できる可能性がある。さらに再発しやすい患者さんには放射線治療ではなく抗がん剤治療を続けるなどの選択肢が提示できる可能性があり、治療を選択する上での指標となりうるという観点で、大きな意義を持つ研究である。

Research Products

• [Journal Article] Liquid Biopsy Cell-free DNA Biomarkers in Patients With Oligometastatic Colorectal Cancer Treated by Ablative Radiotherapy (2021)
  • Author(s): MASAKI NAKAMURA, SHUN-ICHIRO KAGEYAMA, MASAHIDE SEKI, AYAKO SUZUKI, MASAYUKI OKUMURA, HIDEHIRO HOJO, ATSUSHI MOTEGI and TETSUO AKIMOTO
  • Journal Title: ANTICANCER RESEARCH Volume: 41 Issue: 2 Pages: 829-834
  • DOI: 10.21873/anticanres.14835
  • Peer Reviewed / Open Access
• [Journal Article] Additional chemotherapy improved local control and overall survival after stereotactic body radiation therapy for patients with oligo-recurrence. (2018)
  • Author(s): Nakamura M, Hashimoto N, Mayahara H, Uezono H, Harada A, Nishikawa R, Matsuo Y, Kawaguchi H, Nishimura H
  • Journal Title: Radiation Oncology Volume: 13 Issue: 1 Pages: 75-75
  • DOI: 10.1186/s13014-018-1031-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 大腸・直腸癌オリゴメタスタシスの予後予測因子としてのcell free DNA解析 (2021)
  • Author(s): 中村匡希、影山俊一郎、茂木厚、北條秀博、秋元哲夫
  • Organizer: 第49回放射線による制癌シンポジウム
• [Presentation] Identification of Cell-Free DNA profile in oligometastatic colorectal cancer (2020)
  • Author(s): Masaki Nakamura, Shun-ichiro Kageyama, Atsushi Motegi, Hidehiro Hojo, Tetsuo Akimoto
  • Organizer: ESTRO2020
  • Int'l Joint Research