Urinary miRNA biomarker for the diagnosis of colorectal cancer

• Project/Area Number: 18K15757
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 53010:Gastroenterology-related
• Research Institution: Nagoya City University
• Principal Investigator: Kitagawa Mika 名古屋市立大学, 医薬学総合研究院(医学), 助教 (80588632)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 尿 / バイオマーカー / miRNA / 大腸癌 / 診断

Outline of Final Research Achievements

We identified two urinary miRNAs that can detect colorectal cancer (CRC). The panel consisting of two miRNAs could distinguish patients with CRC from HC participants with an area under the curve (AUC) = 0.868 in the independent validation cohort. This urinary biomarker could detect even stage 0/I CRC effectively with an AUC = 0.845. This urinary miRNA biomarker panel showed 82.6% sensitivity and 73.4% specificity for stage 0/I CRC.

Academic Significance and Societal Importance of the Research Achievements

本研究では、内視鏡的切除で治癒可能な超早期の大腸癌をも診断可能な尿中バイオマーカーを同定した。尿はいつでも誰でも簡単に採取可能であることから、本研究成果のバイオマーカーを使用することにより、自宅にいながらの検診が可能となる。また尿検体は無侵襲に採取可能であり、保存容器も限定しないことからコストも安く、今後の大腸癌検診の簡易化・低コスト化に大きく貢献できるものと期待している。

Research Products

• [Journal Article] The first management using intubation of a nasogastric tube with Gastrografin enterography or long tube for non-strangulated acute small bowel obstruction: a multicenter, randomized controlled trial (2020)
  • Author(s): Katano T, Shimura T, Nishie H, Iwai T, Itoh K, Ebi M, Mizuno Y, Togawa S, Shibata S, Yamada T, Mizushima T, Inagaki Y, Kitagawa M, Nojiri Y, Tanaka Y, Okamoto Y, Sugiura M, Matoya S, Nagura Y, Inagaki Y, Koguchi H, Ono S, Ozeki K, Hayashi N, Takiguchi S, Kataoka H
  • Journal Title: Journal of Gastroenterology Volume: 55 Issue: 9 Pages: 858-867
  • DOI: 10.1007/s00535-020-01708-5
  • Peer Reviewed
• [Journal Article] Novel urinary protein biomarker panel for early diagnosis of gastric cancer (2020)
  • Author(s): Shimura T, Dayde D, Wang H, Okuda Y, Iwasaki H, Ebi M, Kitagawa M, Yamada T, Yamada T, Hanash SM, Taguchi A, Kataoka H
  • Journal Title: British Journal of Cancer Volume: 123 Issue: 11 Pages: 1656-1664
  • DOI: 10.1038/s41416-020-01063-5
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Serum Exosomal Dicer Is a Useful Biomarker for Early Detection of Differentiated Gastric Adenocarcinoma (2020)
  • Author(s): Okuda Y, Shimura T, Iwasaki H, Katano T, Kitagawa M, Nishigaki R, Fukusada S, Natsume M, Tanaka M, Nishie H, Ozeki K, Yamada T, Kataoka H.
  • Journal Title: Digestion. Volume: Oct 13 Issue: 4 Pages: 1-10
  • DOI: 10.1159/000510993
  • Peer Reviewed / Open Access
• [Journal Article] A novel urinary microRNA biomarker panel for detecting gastric cancer. (2019)
  • Author(s): Iwasaki H, Shimura T, Yamada T, Okuda Y, Natsume M, Kitagawa M, Horike SI, Kataoka H
  • Journal Title: Journal of Gastroenterology Volume: 54 Issue: 12 Pages: 1061-1069
  • DOI: 10.1007/s00535-019-01601-w
  • Peer Reviewed
• [Journal Article] Placental growth factor is a predictive biomarker for ramucirumab treatment in advanced gastric cancer. (2019)
  • Author(s): Natsume M, Shimura T, Iwasaki H, Okuda Y, Kitagawa M, Okamoto Y, Hayashi K, Kataoka H
  • Journal Title: Cancer Chemother Pharmacol. Volume: Mar 21. Issue: 6 Pages: 1037-1046
  • DOI: 10.1007/s00280-019-03817-2
  • Peer Reviewed / Open Access
• [Journal Article] Urinary Cysteine-Rich Protein 61 and Trefoil Factor 3 as Diagnostic Biomarkers for Colorectal Cancer. (2019)
  • Author(s): Shimura T, Iwasaki H, Kitagawa M, Ebi M, Yamada T, Yamada T, Katano T, Nisie H, Okamoto Y, Ozeki K, Mizoshita T, Kataoka H.
  • Journal Title: Transl Oncol. Volume: 12(3) Issue: 3 Pages: 539-544
  • DOI: 10.1016/j.tranon.2018.12.006
  • Peer Reviewed / Open Access
• [Presentation] 当院における肛門扁平上皮癌に対する治療成績の検討 (2021)
  • Author(s): 北川美香、志村貴也、西垣瑠璃子、尾関貴紀、岩崎弘靖、田中守、西江裕忠、片野敬仁 尾関啓司、久保田英嗣、谷田諭史、片岡洋望
  • Organizer: 第17回日本消化管学会
• [Patent(Industrial Property Rights)] 大腸癌診断用マーカー、大腸癌の診断を補助する方法、大腸癌の診断のためにデータを収集する方法、大腸癌の診断用キット、大腸癌治療薬、大腸癌の治療方法、大腸癌の診断方法 (2020)
  • Inventor(s): 志村貴也、岩崎弘靖、片岡洋望
  • Industrial Property Rights Holder: 志村貴也、岩崎弘靖、片岡洋望
  • Industrial Property Rights Type: 特許
  • Filing Date: 2020
  • Overseas
• [Patent(Industrial Property Rights)] 大腸癌診断用マーカー、大腸癌の診断を補助する方法、大腸癌の診断のた めにデータを収集する方法、大腸癌の診断用キット (2019)
  • Inventor(s): 志村貴也, 岩崎弘靖，片岡洋望
  • Industrial Property Rights Holder: 志村貴也, 岩崎弘靖，片岡洋望
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2019-149338
  • Filing Date: 2019